Western Blot (WB)
(Western Blot using anti-CD98 antibody RL388 (MBS488662). Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 () at 0.05ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rabbit anti-Mouse CD98 Monoclonal Antibody | anti-CD98 antibody
Anti-CD98 [RL388]
Western Blot (WB)
(Western Blot using anti-CD98 antibody RL388 (MBS488662). Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 () at 0.05ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-CD98 antibody RL388 (MBS488662). Mouse fetal liver lysate (35ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric rabbit IgG version of RL388 () at 0.05ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CD98 antibody (MBS488662) RL388. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of RL388 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-CD98 antibody (MBS488662) RL388. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of RL388 () at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
This antibody was raised by immunising rats with an IL 2-producing subline of the mouse T lymphoma EL4.
Note on publication: Describes the original generation of this antibody.
NCBI and Uniprot Product Information
Uniprot Description
SLC3A2: a transmembrane solute carrier protein that transports L-type amino acids and regulates intracellular calcium levels. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. Disulfide-linked heterodimer of a glycosylated heavy chain and a non-glycosylated light chain (SLC7A5, SLC7A6, SLCA7A7, SLC7A8, SLC7A10 or SLCA7A11). Colocalizes with cadherins. Interacts with FAM57A and ICAM1. Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1. Expression is induced in resting peripheral blood T- lymphocytes following PHA stimulation. Expression increases at the time of maximal DNA synthesi, in fibroblasts stimulated to divide. Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid- trimester stage. Belongs to the SLC3A transporter family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Transporter; Membrane protein, integral
Cellular Component: nucleoplasm; cell surface; membrane; cytoplasm; plasma membrane; integral to membrane; nucleus
Molecular Function: cation binding; protein binding; neutral amino acid transmembrane transporter activity; double-stranded RNA binding; catalytic activity
Biological Process: neutral amino acid transport; response to exogenous dsRNA; amino acid transport; transport; carbohydrate metabolic process
Research Articles on CD98
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Product Notes
The CD98 slc3a2 (Catalog #AAA488662) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD98 [RL388] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD98 can be used in a range of immunoassay formats including, but not limited to, stimulatory, Immunoprecipitation (IP), Flow Cytometry (FC/FACS). This antibody has been used in immunoprecipitation analysis to characterise the CD98 antigen (Luscher et al, 1985) and flow cytometric analysis of CD98 expression by gammadelta T cells (Cao et al, 2016). This antibody has been shown to stimulate IL-2 secretion by a T cell lymphoma EL4 subline (EL4-6.1) in the presence of IL-1 (MacDonald et al, 1985). This antibody did not exert stimulatory effects on the parental EL4 line nor a series of ovalbumin-specific T-T hybrids (MacDonald et al, 1985). Researchers should empirically determine the suitability of the CD98 slc3a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD98, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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