Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (MBS488648) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488648, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse IL-6R Monoclonal Antibody | anti-IL-6R antibody
Anti-IL-6R [D7715A7]
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (MBS488648) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488648, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody (MBS488648) D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (MBS488648) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488648, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-6R antibody D7715A7 (MBS488648). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (MBS488648, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow-cytometry using the Anti-IL-6R antibody D7715A7 (MBS488648). Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (MBS488648, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunising Lewis rats with OKT4 cells.
Note on publication: Describes the original generation of this antibody, and the characterisation of its ability to block IL-6 binding.
NCBI and Uniprot Product Information
Uniprot Description
IL6R: Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation may lead to the regulation of the immune response, acute-phase reactions and hematopoiesis. Belongs to the type I cytokine receptor family. Type 3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, cytokine
Cellular Component: apical plasma membrane; cell surface; extracellular region; extracellular space; integral to membrane; interleukin-6 receptor complex; membrane
Molecular Function: ciliary neurotrophic factor receptor activity; enzyme binding; hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-6 binding; interleukin-6 receptor activity; interleukin-6 receptor binding; protein homodimerization activity
Biological Process: cell growth; cytokine and chemokine mediated signaling pathway; developmental growth; endocrine pancreas development; negative regulation of collagen biosynthetic process; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of interleukin-6 production; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of smooth muscle cell proliferation; positive regulation of tyrosine phosphorylation of Stat3 protein; response to cytokine stimulus
Research Articles on IL-6R
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Product Notes
The IL-6R il6ra (Catalog #AAA488649) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL-6R [D7715A7] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-6R can be used in a range of immunoassay formats including, but not limited to, Blocking (BL), Flow Cytometry (FC/FACS), Therapeutics. This antibody binds to IL-6R-positive cells, and blocks IL-6 binding (Vink et al, 1990; Coulie et al, 1990). This antibody is able to inhibit the growth of IL6-dependent plasmacytoma T1033C2 in vitro (Coulie et al, 1990). Administration of this antibody to mice prior to challenge with T1033C2 resulted in a protection rate of 60% and prolonged survival in tumour-bearing mice (Vink et al, 1990). This antibody has been used in flow cytometry to assess IL-6Ra expression (Hoge et al, 2013). When added to dendritic cells subject to CD40 activation in vitro, this antibody neutralises IL-6 and ablates the effect of CD40 activation (Grohmann et al, 2001). Researchers should empirically determine the suitability of the IL-6R il6ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-6R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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