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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1773 results for " variants" - showing 1200-1250
Western Blot (WB)
(Western blot All lanes: Protein canopy homolog 2 antibody at at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 47kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protein canopy homolog 2, Polyclonal Antibody (Cat# AAA1265062)
Full Name
Rabbit anti-human Protein canopy homolog 2 polyclonal Antibody, Biotin conjugated
Gene Names
CNPY2; MSAP; TMEM4; ZSIG9; HP10390
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: C-C motif chemokine 5 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 10 kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 5, Polyclonal Antibody (Cat# AAA719848)
Full Name
Rabbit anti-human C-C motif chemokine 5 polyclonal Antibody, FITC
Gene Names
CCL5; SISd; eoCP; SCYA5; RANTES; TCP228; D17S136E; SIS-delta
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot
(Western Blot analysis using TRIM17 antibodyWestern Blot showing TRIM17 antibody used at a concentration of 1-2 ug/ml to detect its target protein.)
TRIM17, Polyclonal Antibody (Cat# AAA833766)
Full Name
TRIM17 antibody
Gene Names
TRIM17; RBCC; terf; RNF16
Applications
WB
Pricing
$535/0.1 mL | $2,395/5x0.1 mL
Western Blot (WB)
(Western blot All lanes: Protransforming growth factor alpha antibody at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 18 kDa Observed band size: 30 kDa Additional bands at: 35 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protransforming growth factor alpha, Polyclonal Antibody (Cat# AAA719464)
Full Name
Rabbit anti-human Protransforming growth factor alpha polyclonal Antibody, HRP conjugated
Gene Names
TGFA; TFGA
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
PDE6 Alpha, Polyclonal Antibody (Cat# AAA543552)
Full Name
Pan PDE6 Alpha Antibody
Gene Names
PDE6C; COD4; ACHM5; PDEA2
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$490/0.1 mg | $2,065/5x0.1 mg
Western Blot (WB)
(Western blot Alpha-synuclein antibody at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 55 kDa Additional bands at: 118kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Alpha-synuclein, Polyclonal Antibody (Cat# AAA719942)
Full Name
Rabbit anti-human Alpha-synuclein polyclonal Antibody, FITC
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
Endoplasmic Reticulum Aminopeptidase 2, Recombinant Protein (Cat# AAA2028885)
Full Name
Recombinant Endoplasmic Reticulum Aminopeptidase 2 (ERAP2)
Gene Names
ERAP2; LRAP; L-RAP
Applications
PC, WB, May be suitable for use in other assays to be determined by the end user.
Purity
>97%
Pricing
$155/0.01 mg | $285/0.05 mg | $430/0.1 mg | $530/0.2 mg | $1,015/0.5 mg | $1,495/1 mg | $3,660/5 mg | $16,465/5x5 mg
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
kallikrein 6, Polyclonal Antibody (Cat# AAA423217)
Full Name
Goat anti-kallikrein 6 Antibody
Gene Names
KLK6; hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, IHC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Immunohistochemistry (IHC)
(Anti-CA1 / Carbonic Anhydrase I antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)
CA1 / Carbonic Anhydrase I, Polyclonal Antibody (Cat# AAA244737)
Full Name
Goat Polyclonal to Human CA1 / Carbonic Anhydrase I
Gene Names
CA1; CAB; CA-I; Car1; HEL-S-11
Reactivity
Gibbon, Chimpanzee, Gorilla, Human
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
Applications
IHC - Paraffin, WB, EIA
Purity
Immunoaffinity Purified
Pricing
$585/0.05 mg | $2,585/5x0.05 mg
Western Blot (WB)
(Calbindin 2 antibody (MBS5303039) used at 1 ug/ml to detect target protein.)
Calbindin 2, Polyclonal Antibody (Cat# AAA5303039)
Full Name
Calbindin 2 antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Western Blot (WB)
(Western blot analysis of KANK3 in A431 cell lysate with KANK3 antibody at (A) 1 and (B) 2 μg/mL.)
KANK3, Polyclonal Antibody (Cat# AAA153549)
Full Name
KANK3 Antibody
Gene Names
KANK3; ANKRD47
Reactivity
Human, Mouse
Applications
EIA, WB, IHC
Purity
KANK3 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Protein canopy homolog 2 antibody at at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 20 kDa Observed band size: 47kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protein canopy homolog 2, Polyclonal Antibody (Cat# AAA719711)
Full Name
Rabbit anti-human Protein canopy homolog 2 polyclonal Antibody, FITC
Gene Names
CNPY2; MSAP; TMEM4; ZSIG9; HP10390
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Tumor necrosis factor antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 26 kDa Observed band size: 60 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Tumor necrosis factor, Polyclonal Antibody (Cat# AAA719824)
Full Name
Rabbit anti-Bovine Tumor necrosis factor polyclonal Antibody, Biotin conjugated
Gene Names
TNF; TNFa; TNF-a; TNF-alpha
Reactivity
Bovine
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Prodynorphin antibody (MBS839879) used at 1 ug/ml to detect target protein.)
Prodynorphin, Polyclonal Antibody (Cat# AAA839879)
Full Name
Prodynorphin antibody
Gene Names
PDYN; ADCA; PENKB; SCA23
Applications
WB
Purity
Affinity purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Western Blot (WB)
((0. 03ug/ml) staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
TNNI2, Polyclonal Antibody (Cat# AAA423107)
Full Name
Goat anti-TNNI2 Antibody
Gene Names
TNNI2; DA2B; FSSV; fsTnI; AMCD2B
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
PDE4D1, Polyclonal Antibody (Cat# AAA540732)
Full Name
PDE4D1 Antibody
Reactivity
Chicken, Mouse, Rat
Applications
EIA, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
HFE Protein, Polyclonal Antibody (Cat# AAA628825)
Full Name
Anti-HFE Protein (HLA-H)
Gene Names
HFE; HH; HFE1; HLA-H; MVCD7; MGC103790; dJ221C16.10.1
Reactivity
Rat
Applications
ELISA; Western Blot
Purity
Affinity Purified
Pricing
$785/0.1 mg | $3,525/5x0.1 mg
Western Blot (WB)
()
Glutamine Synthetase, Monoclonal Antibody (Cat# AAA838154)
Full Name
Glutamine Synthetase antibody
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Applications
IP
Purity
Glutamine Synthetase antibody was purified by affinity chromatography.
Western Blot (WB)
(Western blot All lanes: Interferon gamma ntibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 18.3 kDaObserved band size: 27 kDa Additional bands at: 70kDa. We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Interferon gamma, Polyclonal Antibody (Cat# AAA715893)
Full Name
Rabbit anti-human Interferon gamma polyclonal Antibody, FITC
Gene Names
IFNG; IFG; IFI
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Diamine acetyltransferase 1 antibody at 2/mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 19 kDa Observed band size: 90 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Diamine acetyltransferase 1, Polyclonal Antibody (Cat# AAA719996)
Full Name
Rabbit anti-human Diamine acetyltransferase 1 polyclonal Antibody, Biotin conjugated
Gene Names
SAT1; SAT; DC21; KFSD; SSAT; KFSDX; SSAT-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
LIG3, Polyclonal Antibody (Cat# AAA1489230)
Full Name
Rabbit Anti Human LIG3 PolyClonal Antibody
Gene Names
LIG3; LIG2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$320/0.1 mL | $1,440/5x0.1 mL
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L17 ntibody at at 2 /ml + EC109whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 58 kDaObserved band size: 30 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
G-protein coupled receptor 161, Polyclonal Antibody (Cat# AAA1265031)
Full Name
Rabbit anti-human G-protein coupled receptor 161 polyclonal Antibody, HRP conjugated
Gene Names
GPR161; RE2
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
Fibulin 4 (FBLN4), Recombinant Protein (Cat# AAA2011933)
Full Name
Recombinant Fibulin 4 (FBLN4)
Gene Names
EFEMP2; MBP1; UPH1; FBLN4; ARCL1B
Reactivity
Homo sapiens (Human)
Applications
WB
Purity
>95%
Pricing
$155/0.01 mg | $280/0.05 mg | $425/0.1 mg | $515/0.2 mg | $990/0.5 mg | $1,460/1 mg | $3,575/5 mg | $16,080/5x5 mg
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L36a-like antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12kDaObserved band size: 19 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L36a, Polyclonal Antibody (Cat# AAA715677)
Full Name
Rabbit anti-human 60S ribosomal protein L36a-like polyclonal Antibody, FITC
Gene Names
RPL36AL; RPL36A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
HBV Pres 1, Monoclonal Antibody (Cat# AAA838576)
Full Name
HBV Pres 1 antibody
Applications
EIA, WB
Pricing
$585/0.1 mg | $2,625/5x0.1 mg
HFE Protein, Peptide (Cat# AAA658123)
Full Name
HFE Protein, Human (HLA-H) Control Peptide
Reactivity
Human
Applications
EL/EIA, WB
Purity
Highly Purified
Highly purified
Highly purified
Pricing
$415/0.1 mg | $1,860/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CRYZL1 protein (Right) or empty vector (Left) detected with CRYZL1 antibody)
CRYZL1, Monoclonal Antibody (Cat# AAA833459)
Full Name
CRYZL1 antibody
Gene Names
CRYZL1; 4P11; QOH-1
Applications
FC/FACS, WB
Purity
CRYZL1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
PDE10A, Polyclonal Antibody (Cat# AAA543414)
Full Name
PDE10A Antibody BIOTIN-Conjugated
Gene Names
PDE10A; ADSD2; IOLOD; HSPDE10A; PDE10A19
Reactivity
Human, Monkey, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(ATP6V1G2 antibody (MBS5301099) used at 0.2-1 ug/ml to detect target protein.)
ATP6V1G2, Polyclonal Antibody (Cat# AAA5301099)
Full Name
ATP6V1G2 antibody
Gene Names
ATP6V1G2; NG38; ATP6G; VMA10; ATP6G2
Applications
WB
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
PDE8A, Polyclonal Antibody (Cat# AAA543567)
Full Name
Pan PDE8A Antibody BIOTIN-Conjugated
Reactivity
Bovine, Human, Monkey, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Plasminogen, Glu, CHOI, Protein (Cat# AAA634508)
Full Name
Plasminogen, Glu, CHOI (Affinity Form I), Human
Purity
Affinity Purified
Native glu-plasminogen is prepared from fresh frozen human plasma by a modification of the procedure of Castellino (3), utilizing gel filtration and affinity chromatography. The two carbohydrate variants of glu-plasminogen (CHOI and C
Native glu-plasminogen is prepared from fresh frozen human plasma by a modification of the procedure of Castellino (3), utilizing gel filtration and affinity chromatography. The two carbohydrate variants of glu-plasminogen (CHOI and C
Pricing
$510/1 mg | $2,285/5x1 mg
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L36a-like antibody at at 2 /mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 12kDaObserved band size: 19 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L36a, Polyclonal Antibody (Cat# AAA715692)
Full Name
Rabbit anti-human 60S ribosomal protein L36a-like polyclonal Antibody, FITC
Gene Names
RPL36AL; RPL36A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot All lanes: Glutathione S-transferase P antibody at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 30 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Glutathione S-transferase P, Polyclonal Antibody (Cat# AAA719485)
Full Name
Rabbit anti-human Glutathione S-transferase P polyclonal Antibody, HRP conjugated
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
BMPR1B, Polyclonal Antibody (Cat# AAA1488294)
Full Name
Rabbit Anti Human BMPR1B PolyClonal Antibody
Gene Names
BMPR1B; ALK6; ALK-6; CDw293
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$325/0.1 mL | $1,465/5x0.1 mL
Estrogen Receptor b1, b2, Polyclonal Antibody (Cat# AAA618404)
Full Name
Anti - Estrogen Receptor b1, b2 (ERb1, 2)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
Purified by immunoaffinity chromatography.
Pricing
$785/0.1 mg | $3,525/5x0.1 mg
Western Blot (WB)
((0.1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
keratin 18, Polyclonal Antibody (Cat# AAA423313)
Full Name
Goat anti-keratin 18 Antibody
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Tested: Human, Pig; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
PDE4D7, Polyclonal Antibody (Cat# AAA543533)
Full Name
PDE4D7 Antibody BIOTIN-Conjugated
Gene Names
Pde4d; Dpde3; 9630011N22Rik
Reactivity
Chicken, Mouse, Rat
Applications
EIA, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(MBS423631 (0.03 μg/ml) staining of Human Heart (A), Kidney (B) and Liver (C) lysate and in Pig Heart (D), Kidney(E) and Liver (F) lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.)
HSP60/HSPD1, Polyclonal Antibody (Cat# AAA423631)
Full Name
Goat anti-HSP60/HSPD1 (aa333-344) Antibody
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Tested: Human, Rat, Pig
Expected from sequence similarity: Human, Dog, Pig, Cow
Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
EIA, WB, IHC, IF
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot of rat cortex lysate showing specific immunolabeling of ~50k Gap-43 protein phosphorylated at Ser41 (Control))
GAP43, Polyclonal Antibody (Cat# AAA835545)
Full Name
GAP43 antibody (Ser41)
Applications
WB
Purity
GAP43 antibody was purified by affinity chromatography
Pricing
$640/0.1 mL | $2,870/5x0.1 mL
Clusterin, Recombinant Protein (Cat# AAA5303991)
Full Name
Clusterin protein
Gene Names
CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
Applications
EIA, WB
Purity
> 90% pure
Pricing
$530/0.1 mg | $2,385/5x0.1 mg
PDE8A, Polyclonal Antibody (Cat# AAA543571)
Full Name
PDE8A Antibody BIOTIN-Conjugated
Reactivity
Bovine, Human, Monkey, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
Sequence
Enhancer Of Zeste Homolog 2 (EZH2), Recombinant Protein (Cat# AAA2097714)
Full Name
Recombinant Enhancer Of Zeste Homolog 2 (EZH2)
Gene Names
EZH2; WVS; ENX1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Homo sapiens (Human)
Applications
SDS, WB
Purity
> 90%
Pricing
$145/0.01 mg | $250/0.05 mg | $365/0.1 mg | $450/0.2 mg | $850/0.5 mg | $1,250/1 mg | $3,055/5 mg | $13,745/5x5 mg
Western Blot (WB)
(ATP8B2 antibody (MBS5300284) used at 1 ug/ml to detect target protein.)
ATP8B2, Polyclonal Antibody (Cat# AAA5300284)
Full Name
ATP8B2 antibody
Gene Names
ATP8B2; ATPID
Applications
WB
Purity
Affinity purified
Pricing
$605/0.1 mL | $2,710/5x0.1 mL
Western Blot (WB)
((2ug/ml) staining of K562 nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
VRK2, Polyclonal Antibody (Cat# AAA423284)
Full Name
Goat anti-VRK2 Antibody
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Biotinylated (0.3ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.)
CD20/MS4A1, Polyclonal Antibody (Cat# AAA423541)
Full Name
Goat anti-CD20/MS4A1 (C Terminus), biotinylated Antibody
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot All lanes: Rhombotin-1 antibody at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 17 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Rhombotin-1, Polyclonal Antibody (Cat# AAA719944)
Full Name
Rabbit anti-human Rhombotin-1 polyclonal Antibody, HRP conjugated
Gene Names
LMO1; TTG1; RBTN1; RHOM1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Testing Data
(C57BL/6 mouse splenocytes stained with purified Ly-49C/F/I/H, followed by anti-Armenian hamster IgG FITC.)
Ly49C,F,H,I, Monoclonal Antibody (Cat# AAA6005291)
Full Name
Ly49C,F,H,I (Killer Cell Lectin-like Receptor 3, 6, 8, 9)
Reactivity
Mouse
Applications
FC/FACS
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$565/0.5 mg | $2,535/5x0.5 mg
Immunoglobulin G (IgG), Native Protein (Cat# AAA2018227)
Full Name
Native Immunoglobulin G (IgG)
Applications
WB, EIA, IP
Purity
> 95%
Pricing
$105/0.01 mg | $155/0.05 mg | $205/0.1 mg | $230/0.2 mg | $415/0.5 mg | $600/1 mg | $1,425/5 mg | $6,410/5x5 mg
SDS-Page
Secernin 1, Recombinant Protein (Cat# AAA2034199)
Full Name
Recombinant Secernin 1 (SCRN1)
Gene Names
SCRN1; SES1
Reactivity
Mus musculus (Mouse)
Applications
WB
Purity
>90%
Pricing
$150/0.01 mg | $260/0.05 mg | $385/0.1 mg | $470/0.2 mg | $900/0.5 mg | $1,320/1 mg | $3,230/5 mg | $14,525/5x5 mg
PDE6 Alpha, Polyclonal Antibody (Cat# AAA543553)
Full Name
Pan PDE6 Alpha Antibody BIOTIN
Gene Names
PDE6C; COD4; ACHM5; PDEA2
Reactivity
Human, Mouse, Rat
Applications
CM, EIA, ICC, IF, IHC, IP, WB
Pricing
$560/0.1 mg | $2,380/5x0.1 mg
