Testing Data
(C57BL/6 mouse splenocytes stained with purified Ly-49C/F/I/H, followed by anti-Armenian hamster IgG FITC.)
Hamster anti-Mouse Ly49C,F,H,I Monoclonal Antibody
Ly49C,F,H,I (Killer Cell Lectin-like Receptor 3, 6, 8, 9)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Ly49C; F; H; I; Monoclonal Antibody; I (Killer Cell Lectin-like Receptor 3; 6; 8; 9); Anti -Ly49C; anti-Ly49C; I antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Specificity
Recognizes an epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants. Reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-Ly49C,F,H,I antibody
Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume
Immunogen
IL-2-activated killer cells (LAK) from C57BL/6 mice
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(C57BL/6 mouse splenocytes stained with purified Ly-49C/F/I/H, followed by anti-Armenian hamster IgG FITC.)
Testing Data
(C57BL/6 mouse splenocytes stained with purified Ly-49C/F/I/H, followed by anti-Armenian hamster IgG FITC.)
Related Product Information for anti-Ly49C,F,H,I antibody
This antibody reacts with a common epitope of Ly-49C/F/I/H family members also known as Ly-49 inhibitory receptors C, F, and I and activating receptor H. It does not react with other Ly-49 members (i.e., Ly-49A, B, D, and G). Ly-49 is a type-II transmembrane, disulfide-linked homodimer with C-type lectin extracellular domains that binds to MHC class I. Ly-49C/F/I/H is expressed on a subset of NK cells, NK-T cells and some CD8+ cells. Ly-49C/F/I, like other members of this family, inhibits NK cell activation through the recognition and interaction of certain MHC class I haplotypes on potential target cells. Ly-49C, Ly-49F, and Ly-49I contain an ITIM motif in their cytoplasmic tails, while Ly-49H lacks ITIM. Ly-49H is encoded by the Cmv1r gene, which promotes resistance to infection by mouse cytomegalovirus (CMV). BALB/c mice are reported to lack the Ly-49H and Ly-49I receptors. This antibody, when bound to NK cells, is capable of inducing redirected cell lysis and blocking NK cell effector function.
Product Categories/Family for anti-Ly49C,F,H,I antibody
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Product Notes
The Ly49C,F,H,I (Catalog #AAA6005291) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The Ly49C,F,H,I (Killer Cell Lectin-like Receptor 3, 6, 8, 9) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ly49C,F,H,I can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry. Dilution: Flow Cytometry: 0.25ug/10e6 cells in 100ul volume. Researchers should empirically determine the suitability of the Ly49C,F,H,I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ly49C,F,H,I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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