Goat CA1 / Carbonic Anhydrase I Polyclonal Antibody | anti-CA1 antibody

Goat Polyclonal to Human CA1 / Carbonic Anhydrase I

Cat. Num. AAA244737

• Gene Names: CA1; CAB; CA-I; Car1; HEL-S-11
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CA1 / Carbonic Anhydrase I; Polyclonal Antibody; Goat Polyclonal to Human CA1 / Carbonic Anhydrase I; Anti-CA1 / Carbonic Anhydrase I Antibody IHC-plus; CA1; CAB; Carbonic anhydrase 1; CAR1; Carbonate dehydratase I; Carbonic dehydratase; CA-I; Carbonic anhydrase B; Carbonic anhydrase I; Human CA1; Carbonic Anhydrase I; anti-CA1 antibody

• Host: Goat
• Reactivity: Gibbon, Chimpanzee, Gorilla, Human; Predicted Reactivity: Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human CA1 / Carbonic Anhydrase I. Reported variants represent identical protein: NP_001729.1, NP_001122303.1, NP_001122302.1, NP_001122301.1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-CA1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:64000), IHC-P (2.5 ug/ml), WB (0.03 - 0.1 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic peptide C-QAIKTKGKRAP from an internal region of human CA1 / Carbonic Anhydrase I (NP_001729.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey, Elephant (91%); Marmoset (82%).
• Immunogen Type: Synthetic peptide
• Immunogen: CA1 / Carbonic Anhydrase I antibody was raised against synthetic peptide C-QAIKTKGKRAP from an internal region of human CA1 / Carbonic Anhydrase I (NP_001729.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon (100%); Monkey, Elephant (91%); Marmoset (82%).
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CA1 / Carbonic Anhydrase I antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

Western Blot (WB)

(Staining (0.03 ug/ml) of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4502517
• NCBI GeneID: 759
• NCBI Accession #: NP_001729.1
• NCBI GenBank Nucleotide #: NM_001738.4
• UniProt Accession #: P00915
• Molecular Weight: 28,870 Da
• NCBI Official Full Name: carbonic anhydrase 1 isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase I
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1; HEL-S-11
• NCBI Protein Information: carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Cation-transporting ATPase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I
• UniProt Entry Name: CAH1_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.

Protein type: Energy Metabolism - nitrogen; Lyase; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process