Secernin 1 Recombinant Protein | SCRN1 recombinant protein

Recombinant Secernin 1 (SCRN1)

Cat. Num. AAA2034199

• Gene Names: SCRN1; SES1
• Reactivity: Mus musculus (Mouse)
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Secernin 1; Recombinant Secernin 1 (SCRN1); SES1; SCRN1 recombinant protein

• Host: E Coli
• Reactivity: Mus musculus (Mouse)
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: 50 ug/ml (varies by lot)
• Sequence Length: 414

• Applicable Applications for SCRN1 recombinant protein: SDS-PAGE, Western Blot (WB), Immunogen, Positive Control
• Residues: Ser2~Lys414
• Tag: Two N-terminal Tags
• Predicted isoelectric point: 4.4
• Usage: Reconstitute in ddH₂O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Phenomenon Explanation: The possble reasons that the actual band size differs from predicted as follows.; 1. Splice Variants - Alternative splicing may create different sized proteins from the same gene.; 2. Relative Charge - The composition of amino acids may affects the charge of the protein.; 3. Post-Translation Modification - Phosphorylation, glycosylation, methylation etc.; 4. Post-Translation Cleavage - Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein - Dimerization, multimerization etc.
• Preparation and Storage: Storage; Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.; ; Stability; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

Sequence

NCBI and Uniprot Product Information

• NCBI GI #: 224465194
• NCBI GeneID: 9805
• NCBI Accession #: NP_001138985.1
• NCBI GenBank Nucleotide #: NM_001145513.1
• Molecular Weight: Predicted Molecular Mass 49.9 kDa; Accurate Molecular Mass 60 kDa
• NCBI Official Full Name: secernin-1 isoform a
• NCBI Official Synonym Full Names: secernin 1
• NCBI Official Symbol: SCRN1
• NCBI Official Synonym Symbols: SES1
• NCBI Protein Information: secernin-1
• UniProt Protein Name: Secernin-1
• Protein Family: Secernin
• UniProt Gene Name: SCRN1
• UniProt Synonym Gene Names: KIAA0193
• UniProt Entry Name: SCRN1_HUMAN

NCBI Description

This gene likely encodes a member of the secernin family of proteins. A similar protein in rat functions in regulation of exocytosis in mast cells. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]

Uniprot Description

SCRN1: Regulates exocytosis in mast cells. Increases both the extent of secretion and the sensitivity of mast cells to stimulation with calcium. Belongs to the peptidase C69 family. Secernin subfamily.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 7p14.3

Cellular Component: cytoplasm; nuclear membrane; nucleus

Molecular Function: dipeptidase activity; protein binding

Biological Process: exocytosis; proteolysis