Rabbit SLC11A2 Polyclonal Antibody | anti-SLC11A2 antibody

SLC11A2, ID (SLC11A2, DCT1, DMT1, NRAMP2, Natural resistance-associated macrophage protein 2, Divalent cation transporter 1, Divalent metal transporter 1) (AP)

Cat. Num. AAA6348011

• Gene Names: SLC11A2; DCT1; DMT1; AHMIO1; NRAMP2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SLC11A2; Polyclonal Antibody; ID (SLC11A2; DCT1; DMT1; NRAMP2; Natural resistance-associated macrophage protein 2; Divalent cation transporter 1; Divalent metal transporter 1) (AP); Divalent metal transporter 1; anti-SLC11A2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLC11A2. Species Crossreactivity: mouse and rat
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 568

• Applicable Applications for anti-SLC11A2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 262-291 in the central region of mouse SLC11A2, UniProt Accession #NP_001167598.1; NP_000608.1; P49281.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(All lanes: MBS6003128 (1:2000) Lane 1: mouse cerebellum lysate Lane 2: rat cerebellum lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 62kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB)

(Western Blot analysis in mouse cerebellum tissue lysates (35ug/lane) usongMBS6003128. This demonstrates that MBS6003128 detected the SLC11A2 protein (arrow).)

Related Product Information for anti-SLC11A2 antibody

This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-SLC11A2 antibody

Antibodies; Transport Proteins

References

1. Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). 2. Ucisik-Akkaya, E., et al. Mol. Hum. Reprod. 16(10):770-777(2010). 3. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 4. Tabuchi, M., et al. J. Cell. Sci. 123 (PT 5), 756-766 (2010). 5. Tabuchi, M., et al. Mol. Biol. Cell 13(12):4371-4387(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 295293171
• NCBI GeneID: 4891
• NCBI Accession #: NP_001167598.1; NP_000608.1
• NCBI GenBank Nucleotide #: NP_001167598.1
• UniProt Accession #: P49281
• NCBI Official Full Name: natural resistance-associated macrophage protein 2 isoform 2
• NCBI Official Synonym Full Names: solute carrier family 11 member 2
• NCBI Official Symbol: SLC11A2
• NCBI Official Synonym Symbols: DCT1; DMT1; AHMIO1; NRAMP2
• NCBI Protein Information: natural resistance-associated macrophage protein 2
• UniProt Protein Name: Natural resistance-associated macrophage protein 2
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: SLC11A2
• UniProt Synonym Gene Names: DCT1; DMT1; NRAMP2; NRAMP 2; DMT-1
• UniProt Entry Name: NRAM2_HUMAN

NCBI Description

This gene encodes a member of the solute carrier family 11 protein family. The product of this gene transports divalent metals and is involved in iron absorption. Mutations in this gene are associated with hypochromic microcytic anemia with iron overload. A related solute carrier family 11 protein gene is located on chromosome 2. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2010]

Uniprot Description

SLC11A2: Important in metal transport, in particular iron. Can also transport manganese, cobalt, cadmium, nickel, vanadium and lead. Involved in apical iron uptake into duodenal enterocytes. Involved in iron transport from acidified endosomes into the cytoplasm of erythroid precursor cells. May play an important role in hepatic iron accumulation and tissue iron distribution. Defects in SLC11A2 are a cause of hypochromic microcytic anemia (HCMA). The disease is characterized by an abnormal hemoglobin content in the erythrocytes which are reduced in size. It may be hereditary or acquired. Mutations in SLC11A2 are associated with progressive liver iron overload and normal to moderately elevated serum ferritin levels. Belongs to the NRAMP family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transporter, SLC family; Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: cell surface; integral to plasma membrane; late endosome membrane; lysosomal membrane; lysosome; early endosome; trans-Golgi network; mitochondrial outer membrane; recycling endosome; membrane; perinuclear region of cytoplasm; apical part of cell; cytoplasm; apical plasma membrane; late endosome; plasma membrane; vacuole; cytoplasmic vesicle; nucleus; brush border

Molecular Function: cadmium ion transmembrane transporter activity; nickel ion transmembrane transporter activity; inorganic cation transmembrane transporter activity; zinc ion binding; hydrogen ion transmembrane transporter activity; manganese ion transmembrane transporter activity; cadmium ion binding; vanadium ion transmembrane transporter activity; nickel ion binding; copper ion transmembrane transporter activity; protein binding; copper ion binding; manganese ion binding; iron ion binding; calcium ion transmembrane transporter activity; zinc ion transmembrane transporter activity; cobalt ion transmembrane transporter activity; lead ion transmembrane transporter activity; ferrous iron transmembrane transporter activity; cobalt ion binding; solute:hydrogen symporter activity

Biological Process: caspase activation; lead ion transport; erythrocyte development; cellular copper ion homeostasis; cellular iron ion homeostasis; dendrite morphogenesis; manganese ion transport; copper ion transport; response to manganese ion; nickel ion transport; response to cadmium ion; learning and/or memory; vanadium ion transport; detection of oxygen; ferrous iron transport; response to lead ion; response to hypoxia; transmembrane transport; response to iron ion; cobalt ion transport; heme biosynthetic process

Disease: Anemia, Hypochromic Microcytic, With Iron Overload 1