HFE Protein Peptide
HFE Protein, Human (HLA-H) Control Peptide
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Highly PurifiedHighly purified
Synonyms
HFE Protein; Human (HLA-H) Control Peptide; HFE Protein peptide
Host
Human
Reactivity
Human
Specificity
100% conserved in human delE3 variant HFE (256 aa); 562-878 variant (242-aa); delE4 variant (334-aa), del#3-7 variant (161 aa), 495-2314del variants (145-aa) and delE3, intron3ins, intron4ins variant (175-aaa
Purity/Purification
Highly Purified
Highly purified
Highly purified
Form/Format
Supplied as a liquid in PBS, pH 7.5
Applicable Applications for HFE Protein peptide
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA. and Antibody Blocking. Not suitable for Western Blot due to low MW: < 3kD.
Dilution: ELISA: Coat with 50-100ng Control Peptide/well
Antibody Blocking: Use 5-10ug per 1ul of H2700 serum or 1ug of H2700-60 affinity purified.
Dilution: ELISA: Coat with 50-100ng Control Peptide/well
Antibody Blocking: Use 5-10ug per 1ul of H2700 serum or 1ug of H2700-60 affinity purified.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for HFE Protein peptide
A 15-aa peptide sequence within the N-terminus of human HFE.
Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. Humans also have very limited capacity to excrete iron. Therefore, cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels at the point of absorption in the intestine and other tissue. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs), iron transporter (NRMAP2/DMT1/DCT1) etc play a key role in iron metabolism. Some genes involved in iron-metabolism are associated with genetic disorders such as Friedreich's Ataxia (Frataxin), genetic hemochromatosis (HFE), and Sex-linked anemia (Hephaestin).
Hereditary hemochromatosis (HHC) is most common autosomal recessive disorder characterized by defective intestinal iron absorption, which lead to iron-overload in many tissues and toxic effects. The candidate gene for HHC encodes the HFE protein (formerly called HLA-H) resembling the major histocompatibility complex MHC class-1 molecule. HFE protein (mouse 359 aa, rat 360 aa, human 348 aa, ~48kD) is type I membrane protein. It is found in all tissues except brain. HFE protein binds too Tfr and reduces its affinity for iron-loaded Tf. The HFE Cys282-Tyr (C282Y) is homozygous in 83-100% of HHC subjects in the US and North Europe, and Australia. The C282Y mutation results in the loss of a structural disulfide bond in the alpha-3 domain of the protein, which prevents association with beta-2 microglobulin and proper presentation to the surface. Defects in HFE are also a cause of porphyria cutanes tarda (PCT), a disorder characterized by light-sensitive dermatitis and presence of large amounts of uroporphyrin in urine.
Elemental iron is required for a variety of normal cellular functions and vital for proper growth and development. However, natural iron is quite insoluble and excess iron is harmful, since it can catalyze the formation of potentially damaging reactive oxygen species. Humans also have very limited capacity to excrete iron. Therefore, cells have developed mechanisms to improve solubility of iron and to control intracellular iron levels at the point of absorption in the intestine and other tissue. Several proteins including Ferritin, transferrin (Tf), transferrin receptors (TfRs), and iron regulatory proteins (IRPs), iron transporter (NRMAP2/DMT1/DCT1) etc play a key role in iron metabolism. Some genes involved in iron-metabolism are associated with genetic disorders such as Friedreich's Ataxia (Frataxin), genetic hemochromatosis (HFE), and Sex-linked anemia (Hephaestin).
Hereditary hemochromatosis (HHC) is most common autosomal recessive disorder characterized by defective intestinal iron absorption, which lead to iron-overload in many tissues and toxic effects. The candidate gene for HHC encodes the HFE protein (formerly called HLA-H) resembling the major histocompatibility complex MHC class-1 molecule. HFE protein (mouse 359 aa, rat 360 aa, human 348 aa, ~48kD) is type I membrane protein. It is found in all tissues except brain. HFE protein binds too Tfr and reduces its affinity for iron-loaded Tf. The HFE Cys282-Tyr (C282Y) is homozygous in 83-100% of HHC subjects in the US and North Europe, and Australia. The C282Y mutation results in the loss of a structural disulfide bond in the alpha-3 domain of the protein, which prevents association with beta-2 microglobulin and proper presentation to the surface. Defects in HFE are also a cause of porphyria cutanes tarda (PCT), a disorder characterized by light-sensitive dermatitis and presence of large amounts of uroporphyrin in urine.
Product Categories/Family for HFE Protein peptide
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
HFE protein
Similar Products
Product Notes
The HFE Protein (Catalog #AAA658123) is a Peptide produced from Human and is intended for research purposes only. The product is available for immediate purchase. The HFE Protein, Human (HLA-H) Control Peptide reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HFE Protein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA. and Antibody Blocking. Not suitable for Western Blot due to low MW: < 3kD. Dilution: ELISA: Coat with 50-100ng Control Peptide/well Antibody Blocking: Use 5-10ug per 1ul of H2700 serum or 1ug of H2700-60 affinity purified. Researchers should empirically determine the suitability of the HFE Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HFE Protein, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
