Western Blot (WB)
(Western blot Alpha-synuclein antibody at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 55 kDa Additional bands at: 118kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Rabbit anti-Human Alpha-synuclein Polyclonal Antibody | anti-SNCA antibody
Rabbit anti-human Alpha-synuclein polyclonal Antibody, FITC
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Western Blot (WB)
(Western blot Alpha-synuclein antibody at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 55 kDa Additional bands at: 118kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Western Blot (WB)
(Western blot Alpha-synuclein antibody at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 55 kDa Additional bands at: 118kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
NCBI and Uniprot Product Information
NCBI Description
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: actin cytoskeleton; axon; cell cortex; cell junction; cytoplasm; cytosol; extracellular region; extracellular space; fibril; Golgi apparatus; growth cone; inclusion body; lysosome; membrane; mitochondrial respiratory chain complex I; mitochondrion; nuclear outer membrane; nucleus; perinuclear region of cytoplasm; plasma membrane; platelet alpha granule membrane; ribosome; rough endoplasmic reticulum; synaptic vesicle; terminal button
Molecular Function: alpha-tubulin binding; beta-tubulin binding; calcium ion binding; caspase inhibitor activity; copper ion binding; dynein binding; fatty acid binding; ferrous iron binding; histone binding; identical protein binding; kinesin binding; magnesium ion binding; microtubule binding; oxidoreductase activity; phospholipase binding; phospholipid binding; phosphoprotein binding; protein binding; protein domain specific binding; protein N-terminus binding; tau protein binding; zinc ion binding
Biological Process: adult locomotory behavior; aging; behavioral response to cocaine; calcium ion homeostasis; caspase activation; cellular protein metabolic process; dopamine biosynthetic process; dopamine uptake; fatty acid metabolic process; fibril organization and biogenesis; microglial cell activation; mitochondrial membrane organization and biogenesis; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of dopamine metabolic process; negative regulation of dopamine uptake; negative regulation of exocytosis; negative regulation of histone acetylation; negative regulation of microtubule polymerization; negative regulation of monooxygenase activity; negative regulation of neuron apoptosis; negative regulation of norepinephrine uptake; negative regulation of protein amino acid phosphorylation; negative regulation of serotonin uptake; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transporter activity; neutral lipid metabolic process; organelle ATP synthesis coupled electron transport; phospholipid metabolic process; positive regulation of apoptosis; positive regulation of endocytosis; positive regulation of neurotransmitter secretion; positive regulation of peptidyl-serine phosphorylation; positive regulation of receptor recycling; positive regulation of release of sequestered calcium ion into cytosol; protein destabilization; receptor internalization; regulation of acyl-CoA biosynthetic process; regulation of dopamine secretion; regulation of excitatory postsynaptic membrane potential; regulation of glutamate secretion; regulation of locomotion; regulation of long-term neuronal synaptic plasticity; regulation of macrophage activation; response to drug; response to iron(II) ion; response to lipopolysaccharide; response to magnesium ion; synapse organization and biogenesis; synaptic vesicle endocytosis
Disease: Dementia, Lewy Body; Parkinson Disease 1, Autosomal Dominant; Parkinson Disease 4, Autosomal Dominant
Research Articles on SNCA
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Product Notes
The SNCA snca (Catalog #AAA719942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Alpha-synuclein polyclonal Antibody, FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-synuclein can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the SNCA snca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-synuclein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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