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2006 results for "Goat IgG Isotype Control" - showing 900-950
Ig Fab, Secondary Antibody (Cat# AAA679301)
Full Name
Goat Anti-Human Ig Fab-FITC
Reactivity
Human IgG Fc; may react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on human pooled immunoglobulins covalently linked to agarose
Pricing
$245/0.5 mg | $960/5x0.5 mg
Testing Data
Kappa, Antibody (Cat# AAA670603)
Full Name
Goat Anti-Human Kappa, Mouse ads-FITC
Applications
EIA, FC/FACS
Purity
Affinity chromatography on pooled human kappa light chains covalently linked to agarose
Pricing
$350/1 mg | $1,420/5x1 mg
Goat Anti-Rabbit IgG Fab, Secondary Antibody (Cat# AAA670688)
Full Name
Goat Anti-Rabbit IgG Fab-BIOT
Applications
EIA
Purity
Affinity chromatography on pooled rabbit IgG covalently linked to agarose
Pricing
$180/0.5 mg | $665/5x0.5 mg
Western Blot (WB)
(Western BlotPositive WB detected in: 3 different overexpression lysates with HA taggedAll lanes: HA-Tag antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 35, 35, 48 kDaObserved band size: 35, 35, 48 kDa)
HA-Tag, Monoclonal Antibody (Cat# AAA7135631)
Full Name
HA-Tag Monoclonal Antibody
Reactivity
All
Applications
EIA, WB, IF, FC, IP
Purity
>95%, Protein A purified
Pricing
$150/0.05 mL | $200/0.1 mL | $900/5x0.1 mL
Testing Data
(Figure A. FITC conjugated Rat anti Mouse CD4 and Amethyst Orange conjugated Rat IgG2a isotype control . Figure B. FITC conjugated Rat anti Mouse CD4 and Amethyst Orange conjugated Rat anti Mouse CD8a . All experiments performed on mouse blood gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer)
CD8 Alpha, Monoclonal Antibody (Cat# AAA225861)
Full Name
Rat Anti Mouse CD8 Alpha: Amethyst Orange
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
$500/0.1 mg | $2,090/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of uPA Receptor using anti-uPA Receptor antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysate,Lane 2: human U20S whole cell lysate,Lane 3: human A431 whole cell lysate,Lane 4: human Hela whole cell lysate,Lane 5: human A549 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-uPA Receptor antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for uPA Receptor at approximately 39KD. The expected band size for uPA Receptor is at 37KD.)
uPA Receptor/PLAUR, Polyclonal Antibody (Cat# AAA1751299)
Full Name
Anti-uPA Receptor/PLAUR Antibody
Gene Names
PLAUR; CD87; UPAR; URKR; U-PAR
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human IDS antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human IDS )
Iduronate 2-Sulfatase/IDS, Monoclonal Antibody (Cat# AAA200476)
Full Name
Iduronate 2-Sulfatase/IDS
Gene Names
IDS; ID2S; MPS2; SIDS
Reactivity
Human
Applications
EIA, WB, FC/FACS
Purity
Protein-A Affinity Chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of Osteocalcin using anti- Osteocalcin antibody (MBS178419). Osteocalcin was detected in paraffin-embedded section of mouse tibia tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- Osteocalcin Antibody (MBS178419) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Osteocalcin, Polyclonal Antibody (Cat# AAA178419)
Full Name
Anti-Osteocalcin Antibody
Gene Names
Bglap; OC; BGP; OG1; mOC-A; Bglap1
Reactivity
Mouse
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Serum Albumin, Antibody (Cat# AAA670625)
Full Name
Goat Anti-Bovine Serum Albumin, Human Serum Albumin ads-BIOT
Gene Names
ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
Applications
EIA
Purity
Affinity chromatography on bovine serum albumin covalently linked to agarose
Pricing
$220/1 mg | $830/5x1 mg
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KIR/CD158 antibody (1:10000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;Lane 1.: 293T cell lysate;Lane 2.: MCF7 cell lysate;Lane 3.: HepG2 cell lysate;Lane 4.: K562 cell lysate)
KIR/CD158, Monoclonal Antibody (Cat# AAA200488)
Full Name
KIR/CD158
Gene Names
KIR2DL5A; CD158F; KIR2DL5; KIR2DL5.1; KIR2DL5.3
Reactivity
Human
Applications
EIA, WB, FC/FACS
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of SUR1 using anti-SUR1 antibody (MBS1750507).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUR1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SUR1 at approximately 177KD. The expected band size for SUR1 is at 177KD. )
SUR1, Polyclonal Antibody (Cat# AAA1750507)
Full Name
Anti-SUR1 Picoband antibody
Gene Names
ABCC8; HI; SUR; HHF1; MRP8; PHHI; SUR1; ABC36; HRINS; TNDM2; SUR1delta2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TNF Receptor I? using anti-TNF Receptor I? antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysate,Lane 2: human K562 whole cell lysate,Lane 3: human Caco-2 whole cell lysate,Lane 4: rat liver tissue lysates,Lane 5: mouse small intestine tissue lysates,Lane 6: mouse liver tissue lysates,Lane 7: mouse HEPA1-6 whole cell lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor I? antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TNF Receptor I? at approximately 50KD. The expected band size for TNF Receptor I? is at 50KD.)
TNF Receptor I/TNFRSF1A, Polyclonal Antibody (Cat# AAA1751231)
Full Name
Anti-TNF Receptor I/TNFRSF1A Antibody
Gene Names
TNFRSF1A; FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG2a, Monoclonal Antibody (Cat# AAA666886)
Full Name
Mouse IgG2a
Applications
EIA, FC/FACS, IHC
Purity
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Immunoglobulin was enzymaticly cleaved using Immobilized Pepsin. F(ab')2 was separated from intact antibody and Fc fragments by Protein A and Size exclusion chromatograp
Pricing
$650/0.1 mg | $2,880/5x0.1 mg
Serum Albumin, Antibody (Cat# AAA670614)
Full Name
Goat Anti-Human Serum Albumin, Bovine Serum Albumin ads-BIOT
Gene Names
ALB; FDAH; ANALBA; PRO0883; PRO0903; PRO1341
Applications
EIA
Purity
Affinity chromatography on human serum albumin covalently linked to agarose
Pricing
$220/1 mg | $830/5x1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22316a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22316a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
NAT10, Polyclonal Antibody (Cat# AAA9231254)
Full Name
NAT10 Antibody (N-Term)
Gene Names
NAT10; ALP; Kre33; NET43
Reactivity
Human
Predicted: Mouse
Predicted: Mouse
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
. Section was blocked with 10% normal goat serum 30min at 37°C Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. MBS9018332_IHC.jpg)
Western Blot (WB)
( Western Blot Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysateAll lanes TUBB antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 55 KDaObserved band size: 55 KDaExposure time:5s)
TUBB, Monoclonal Antibody (Cat# AAA9018332)
Full Name
TUBB Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein A purified
Pricing
$150/0.05 mL | $200/0.1 mL | $900/5x0.1 mL
IgG2a, Monoclonal Antibody (Cat# AAA666889)
Full Name
Mouse IgG2a
Purity
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Immunoglobulin was enzymaticly cleaved using Immobilized Pepsin. Fab monomer was separated from intact antibody, F(ab')2 and Fc fragments by Protein A and Size exclusion
Pricing
$650/0.1 mg | $2,880/5x0.1 mg
Western Blot (WB)
TUBA1A, Antibody (Cat# AAA7135645)
Full Name
TUBA1A
Gene Names
Tuba1a; Tuba1; Tuba-1
Reactivity
Human, Rabbit, Rat, Mouse
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Pricing
$150/0.05 mL | $200/0.1 mL | $900/5x0.1 mL
Western Blot (WB)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling SYIM with M25221 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
IARS2/SYIM, Monoclonal Antibody (Cat# AAA435131)
Full Name
IARS2/SYIM (272) Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CCR3 using anti-CCR3 antibody (MBS1750708).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human U-87MG whole cell lysates,Lane 5: human CCRF-CEM whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR3 at approximately 55KD. The expected band size for CCR3 is at 41KD. )
CCR3, Polyclonal Antibody (Cat# AAA1750708)
Full Name
Anti-CCR3 Picoband antibody
Gene Names
CCR3; CKR3; CD193; CMKBR3; CC-CKR-3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
FC/FACS, IHC, ICC, WB
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western Blot: Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysateAll lanes: PKM antibody at 1:4000Secondary: Goat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 58 kDaObserved band size: 58 KDaExposure time: 1min)
PKM, Monoclonal Antibody (Cat# AAA7135948)
Full Name
PKM Monoclonal Antibody
Reactivity
Human, Rat, Mouse, Rabbit
Applications
EIA, WB, IHC, IF, FC/FACS, IP
Purity
>95%, Protein G purified
Pricing
$205/0.05 mL | $290/0.1 mL | $1,305/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CLCN1, Polyclonal Antibody (Cat# AAA9231248)
Full Name
CLCN1 Antibody (N-Term)
Gene Names
CLCN1; CLC1
Reactivity
Human, Mouse
Applications
FC, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Testing Data
IgG1, Secondary Antibody (Cat# AAA679278)
Full Name
Goat Anti-Mouse IgG1, Human/Bovine/Horse SP ads-FITC
Reactivity
Mouse IgG2a, IgG2b, IgG3, IgM, and IgA; human, bovine, and horse serum proteins (SP); may react with immunoglobulins from other species
Applications
EIA
Purity
Affinity chromatography on mouse IgG1 covalently linked to agarose
Pricing
$310/1 mg | $1,240/5x1 mg
Testing Data
IgG Fab, Secondary Antibody (Cat# AAA679273)
Full Name
Goat Anti-Mouse IgG Fab-BIOT
Reactivity
Mouse IgG Fc, IgM, and IgA; may react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on mouse IgG Fab covalently linked to agarose
Pricing
$180/1 mg | $665/5x1 mg
Immunoprecipitation (IP)
(ACOD was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25062 of 10 ug. Western blot was performed from the immunopre-cipitate using M25062 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 41 KDaObserved band size : 41 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
SCDOS, Monoclonal Antibody (Cat# AAA435100)
Full Name
SCDOS Mouse mAb
Gene Names
SCD; SCD1; FADS5; SCDOS; hSCD1; MSTP008
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(NO66(652) was immunopre-cipitated from 1mg of Jurkat cells membrane fraction, blotted with M25089 of 10 ug. Western blot was performed from the immunopre-cipitate using M25089 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 71 KDaObserved band size : 71 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
NO66, Monoclonal Antibody (Cat# AAA435174)
Full Name
NO66 (652) Mouse mAb
Gene Names
RIOX1; ROX; NO66; JMJD9; MAPJD; URLC2; hsNO66; C14orf169
Reactivity
Human
Applications
WB, FC/FACS, IP
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22304c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22304c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MYL2, Polyclonal Antibody (Cat# AAA9231240)
Full Name
MYL2 Antibody (Center)
Gene Names
MYL2; MLC2; CMH10; MLC-2s/v
Reactivity
Human, Mouse, Rat
Predicted: Chicken, Rabbit
Predicted: Chicken, Rabbit
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Testing Data
(Cell Surface flow analysis of mouse CD3 in splenocytes using 0.5 μg/10^6 cells. Green represents isotype control; red represents mouse CD3 antibody. Goat anti-hamster IgG FITC conjugate secondary antibody was used. )
CD3 zeta, Monoclonal Antibody (Cat# AAA179846)
Full Name
Anti-CD3 zeta Rabbit Monoclonal Antibody
Gene Names
CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; IMD25; CD3-ZETA
Reactivity
Human
Applications
FC/FACS, IF, ICC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: rat spleen tissue lysatesLane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Carbonic Anhydrase I/CA1 antigen affinity purified monoclonal antibody (Catalog # MBS1753963) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 29KD. The expected band size for Carbonic Anhydrase I/CA1 is at 29KD. )
Carbonic Anhydrase I/CA1, Monoclonal Antibody (Cat# AAA1753963)
Full Name
Anti-Carbonic Anhydrase I/CA1 Antibody (monoclonal, 4D5)
Gene Names
CA1; CAB; CA-I; Car1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SCARB1 using anti-SCARB1 antibody (MBS178336).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Testis Tissue Lysate,Lane 2: Mouse Testis Tissue Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SCARB1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SCARB1 at approximately 57KD. The expected band size for SCARB1 is at 57KD. )
SCARB1, Polyclonal Antibody (Cat# AAA178336)
Full Name
Anti-SCARB1 Antibody
Gene Names
Scarb1; CD36; Cla1; SRBI; Srb1; Cla-1; Hdlq1; SR-B1; SR-BI; Cd36l1; Chohd1; Hlb398; mSR-BI; AI120173; D5Ertd460e
Reactivity
Mouse, Rat
Applications
WB
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Figure A. RPE conjugated Mouse anti Bovine CD45RO Antibody and FITC conjugated Mouse IgG2a isotype control . Figure B. RPE conjugated Mouse anti Bovine CD45RO Antibody and FITC conjugated Mouse anti Bovine WC1 Antibody . All experiments performed on red cell lysed bovine blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.)
WC1, Monoclonal Antibody (Cat# AAA225719)
Full Name
Mouse Anti Bovine WC1
Gene Names
CD163L1; WC1; WC1-2; WC1-5; WC1.1
Reactivity
Bovine, Sheep, Goat
Applications
IHC, FC/FACS, IP
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22348a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22348a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
ATP6V1G3, Polyclonal Antibody (Cat# AAA9231296)
Full Name
ATP6V1G3 Antibody (N-Term)
Gene Names
ATP6V1G3; Vma10; ATP6G3
Reactivity
Human, Mouse
Predicted: Xenopus
Predicted: Xenopus
Applications
IF, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunohistochemistry (IHC)
(Figure 1. IHC analysis of ICOS using anti-ICOS antibody (MBS1750366).
ICOS was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ICOS Antibody (MBS1750366) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
ICOS, Polyclonal Antibody (Cat# AAA1750366)
Full Name
Anti-ICOS Picoband antibody
Gene Names
ICOS; AILIM; CD278; CVID1
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of TCR alpha using anti-TCR alpha antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TCR alpha protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCR alpha antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCR alpha at approximately 16KD. The expected band size for TCR alpha is at 16KD.)
TCR alpha/TRAC, Polyclonal Antibody (Cat# AAA1751487)
Full Name
Anti-TCR alpha/TRAC Antibody
Gene Names
TRAC; TRA; IMD7; TCRA; TRCA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
IgG1, Secondary Antibody (Cat# AAA679276)
Full Name
Goat Anti-Mouse IgG1-FITC
Reactivity
Mouse IgG2a, IgG2b, IgG3, IgM, and IgA; may react with immunoglobulins from other species
Applications
EIA, FLISA, ICC
Purity
Affinity chromatography on mouse IgG1 covalently linked to agarose
Pricing
$260/1 mg | $1,015/5x1 mg
Testing Data
IgG Fab, Secondary Antibody (Cat# AAA679270)
Full Name
Goat Anti-Mouse IgG Fab-FITC
Reactivity
Mouse IgG Fc, IgM, and IgA; may react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on mouse IgG Fab covalently linked to agarose
Pricing
$180/1 mg | $665/5x1 mg
IgG, Secondary Antibody (Cat# AAA679307)
Full Name
Swine Anti-Rabbit IgG (H+L), Multi-Species SP ads-UNLB
Reactivity
Rabbit, Mouse, rat, human, goat, hamster, bovine, horse, sheep, chicken, and guinea pig serum protein (SP); may react with immunoglobulins from other species and the light chains of other rabbit immunoglobulins
Applications
EIA
Purity
Affinity chromatography on rabbit IgG covalently linked to agarose
Pricing
$200/1 mg | $755/5x1 mg
Ig Fc, Secondary Antibody (Cat# AAA679299)
Full Name
Goat Anti-Human Ig Fc-BIOT
Reactivity
Human IgG Fab; may react with immunoglobulins from other species and the Fc region of other human immunoglobulins
Applications
EIA
Purity
Affinity chromatography
Pricing
$185/0.5 mg | $680/5x0.5 mg
Testing Data
IgG Fc, Secondary Antibody (Cat# AAA679292)
Full Name
Goat Anti-Human IgG Fc, Multi-Species SP ads-PE
Reactivity
Human IgG Fab, IgM, and IgA; rabbit, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum proteins (SP); may react with IgG from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on human IgG Fc covalently linked to agarose
Pricing
$335/0.25 mg | $1,370/5x0.25 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Bovine CD21 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Bovine CD21 and Mouse anti Bovine WC4 detected with Goat anti Mouse IgG1:RPE . All experiments performed on bovine red blood cell lysed blood gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
WC4, Monoclonal Antibody (Cat# AAA225844)
Full Name
Mouse Anti Bovine WC4
Reactivity
Bovine
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A.
Pricing
$405/0.1 mg | $1,660/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing huuman peripheral blood monocytes stained with CD14 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CD14, Monoclonal Antibody (Cat# AAA9231265)
Full Name
Human CD14 Antibody
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
IgM, Secondary Antibody (Cat# AAA679275)
Full Name
Goat F (ab')2 Anti-Mouse IgM-LE/AF
Reactivity
Mouse IgG and IgA; may react with immunoglobulins from other species
Applications
EIA
Pricing
$260/0.5 mg | $1,015/5x0.5 mg
Immunofluorescence (IF)
(Figure 2. IF analysis of CDK12 using anti-CDK12 antibody (MBS1753598).CDK12 was detected in immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (MBS176582) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- CDK12 Antibody (MBS1753598) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used. )
CDK12, Polyclonal Antibody (Cat# AAA1753598)
Full Name
Anti-CDK12 Antibody
Gene Names
CDK12; CRK7; CRKR; CRKRS
Reactivity
Human
Applications
WB, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of Lactate Dehydrogenase A/LDHA in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
Lactate Dehydrogenase A/LDHA, Monoclonal Antibody (Cat# AAA201216)
Full Name
Lactate Dehydrogenase A/LDHA
Gene Names
LDHA; LDHM; GSD11; PIG19; HEL-S-133P
Reactivity
Human
Applications
EIA, WB, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Anti-IL1RAP Antibody (Center) at 1:2000 dilution + Mouse liver lysateLysates/proteins at 20 µg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IL1RAP, Polyclonal Antibody (Cat# AAA9232320)
Full Name
IL1RAP Antibody (Center)
Gene Names
IL1RAP; IL1R3; C3orf13; IL-1RAcP
Reactivity
Human, Mouse
Applications
WB, FC/FACS, IHC
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808380)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: ATTO 594
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,385/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CKS2, Polyclonal Antibody (Cat# AAA9231257)
Full Name
CKS2 Antibody (N-Term)
Gene Names
CKS2; CKSHS2
Reactivity
Human
Predicted: Bovine, Mouse
Predicted: Bovine, Mouse
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of cell line lysates (35ug/lane) using MBS6480911. HLA-B (arrow) was detected using MBS6480911.)
HLA Class 1 Antigen B, Antibody (Cat# AAA6480911)
Full Name
HLA Class 1 Antigen B, NT (HLA Cass I Histocompatibility Antigen B-27 alpha Chain, HLA B Class I, HLA-B, HLAB, HLA-B27, HLA-B73, AS, MGC111087, MHC Class I Antigen B*27, SPDA1) (Biotin)
Gene Names
HLA-B; AS; HLAB; SPDA1
Applications
EIA, WB, IHC
Purity
Purified by Protein A and peptide affinity chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Testing Data
IgG3, Secondary Antibody (Cat# AAA679281)
Full Name
Goat Anti-Mouse IgG3-FITC
Reactivity
Mouse IgG1, IgG2a, IgG2b, IgM, and IgA; may react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on mouse IgG3 covalently linked to agarose
Pricing
$260/1 mg | $1,015/5x1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22343b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22343b, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
KCNH1, Polyclonal Antibody (Cat# AAA9231291)
Full Name
KCNH1 Antibody (C-Term)
Gene Names
KCNH1; EAG; EAG1; ZLS1; TMBTS; h-eag; hEAG1; Kv10.1
Reactivity
Human
Predicted: Human
Predicted: Human
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
