Testing Data
(Cell Surface flow analysis of mouse CD3 in splenocytes using 0.5 μg/10^6 cells. Green represents isotype control; red represents mouse CD3 antibody. Goat anti-hamster IgG FITC conjugate secondary antibody was used. )
Rabbit anti-Human CD3 zeta Monoclonal Antibody | anti-CD3 zeta antibody
Anti-CD3 zeta Rabbit Monoclonal Antibody
FC/FACS: 1:20
Testing Data
(Cell Surface flow analysis of mouse CD3 in splenocytes using 0.5 μg/10^6 cells. Green represents isotype control; red represents mouse CD3 antibody. Goat anti-hamster IgG FITC conjugate secondary antibody was used. )
Testing Data
(Cell Surface flow analysis of mouse CD3 in splenocytes using 0.5 μg/10^6 cells. Green represents isotype control; red represents mouse CD3 antibody. Goat anti-hamster IgG FITC conjugate secondary antibody was used. )
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) ().
Research Articles on CD3 zeta
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Product Notes
The CD3 zeta cd247 (Catalog #AAA179846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD3 zeta Rabbit Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 zeta can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunocytochemistry (ICC). ICC/IF 1:100-1:200 FC/FACS: 1:20. Researchers should empirically determine the suitability of the CD3 zeta cd247 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 zeta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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