Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
2006 results for "Goat IgG Isotype Control" - showing 950-1000
Western Blot (WB)
(Figure 1. Western blot analysis of STIL/SIL using anti-STIL/SIL antibody (MBS1753683).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat testis tissue lysatesLane 2: rat C6 whole cell lysatesLane 3: rat PC-12 whole cell lysatesLane 4: mouse NIH/3T3 whole cell lysatesLane 5: human Hela whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-STIL/SIL antigen affinity purified polyclonal antibody (Catalog # MBS1753683) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460 ) with Tanon 5200 system. A specific band was detected for STIL/SIL at approximately 170KD. The expected band size for STIL/SIL is at 170KD. )
STIL/SIL, Polyclonal Antibody (Cat# AAA1753683)
Full Name
Anti-STIL/SIL Antibody
Gene Names
STIL; SIL; MCPH7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunoprecipitation (IP)
(BAK was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25232 of 10 ug. Western blot was performed from the immunoprecipitate using M25232 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Predicted band size : 24 KDaObserved band size : 24 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
BAK, Monoclonal Antibody (Cat# AAA435121)
Full Name
BAK Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808376)
Full Name
N-terminal Arginylation Antibody, Clone 4A9
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,160/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (MBS1750334).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: mouse spleen tissue lysates,Lane 4: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD. )
CD11b, Polyclonal Antibody (Cat# AAA1750334)
Full Name
Anti-CD11b Picoband antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (MBS1753594).ApoER2/LRP8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (MBS1753594) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
ApoER2/LRP8, Polyclonal Antibody (Cat# AAA1753594)
Full Name
Anti-ApoER2/LRP8 Antibody
Gene Names
LRP8; MCI1; LRP-8; APOER2; HSZ75190
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22340a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22340a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MGEA5, Polyclonal Antibody (Cat# AAA9231287)
Full Name
MGEA5 Antibody (N-Term)
Gene Names
MGEA5; OGA; MEA5; NCOAT
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
IHC, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Testing Data
(Figure 1:Apoptosis induced in Jurkat T leukemia cells by Killer TRAILTM (MBS566041) is completely blocked by mouse MAb to TRAIL (human) (2E5) in a concentration range of 0.5-1.5µg/ml. Concentration of MAb 2E5 required for inhibition may vary depending of the cell type studied and the concentration of soluble recombinant human TRAIL used. The neutralizing activity of MAb 2E5 has been confirmed with various sources of soluble recombinant human TRAIL including KillerTRAILTM. Method: Jurkat cells (5x104) were treated with KillerTRAILTM (300ng/ml) in the presence or absence of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.")
TRAIL, Monoclonal Antibody (Cat# AAA566327)
Full Name
TRAIL (human) monoclonal antibody (2E5)
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
Reactivity
Human
Does not cross-react with mouse TRAIL
Does not cross-react with mouse TRAIL
Applications
FC/FACS, FUNC
Purity
Protein A-Affinity purified Sterile filtered
Pricing
$535/0.1 mg | $2,350/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Tigit using anti-Tigit antibody (MBS1753480).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysatesLane 2: rat plasma lysatesLane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Tigit antigen affinity purified polyclonal antibody (Catalog # MBS1753480) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Tigit at approximately 30KD. The expected band size for Tigit is at 30KD. )
Tigit, Polyclonal Antibody (Cat# AAA1753480)
Full Name
Anti-Tigit Antibody
Gene Names
Tigit; Vstm3
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing PHA-stimulated huuman peripheral blood lymphocytes stained with CD25 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2a (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CD25, Monoclonal Antibody (Cat# AAA9231269)
Full Name
Human CD25 Antibody
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of PC-3 cells using anti-AMPK beta 2 antibody (M05077).Overlay histogram showing PC-3 cells stained with M05077 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-AMPK beta 2 Antibody (M05077, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AMPK beta 2, Monoclonal Antibody (Cat# AAA1752346)
Full Name
Anti-AMPK beta 2 Antibody (monoclonal, 6G1)
Reactivity
Human
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808391)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: PerCP
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,380/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of KCNQ2 using anti-KCNQ2 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNQ2 at approximately 97 kDa. The expected band size for KCNQ2 is at 97 kDa.)
KCNQ2, Polyclonal Antibody (Cat# AAA1753421)
Full Name
Anti-KCNQ2 Antibody
Gene Names
KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808385)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$575/0.1 mg | $2,355/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing huuman peripheral blood lymphocytes stained with CD7 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CD7, Monoclonal Antibody (Cat# AAA9231264)
Full Name
Human CD7 Antibody
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (MBS1753563).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: rat PC-12 whole cell lysatesLane 3: mouse heart tissue lysatesLane 4: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # MBS1753563) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 51KD. The expected band size for Integrin linked ILK is at 51KD. )
Integrin linked ILK, Polyclonal Antibody (Cat# AAA1753563)
Full Name
Anti-Integrin linked ILK Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG, Secondary Antibody (Cat# AAA679319)
Full Name
Goat Anti-Donkey IgG (H+L)-BIOT
Reactivity
Donkey; May react with immunoglobulins from other species and the light chains of other equine immunoglobulins
Applications
EIA
Purity
Affinity chromatography on donkey IgG covalently linked to agarose
Pricing
$160/1 mg | $570/5x1 mg
Testing Data
IgG2a, Secondary Antibody (Cat# AAA679279)
Full Name
Goat Anti-Mouse IgG2a, Human/Bovine/Horse SP ads-FITC
Reactivity
Mouse IgM, IgG1, IgG2b, IgG3, IgA, and human, bovine, and horse serum proteins
Applications
EIA, FLISA
Purity
Affinity chromatography on mouse IgG2a covalently linked to agarose
Pricing
$310/1 mg | $1,240/5x1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of HEPG2 cells using anti-Human PNP antibody.
Overlay histogram showing HEPG2 cells stained with A00988-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human PNP Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
PNP, Polyclonal Antibody (Cat# AAA1751296)
Full Name
Anti-Human PNP DyLight 488 conjugated Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD11a using anti-CD11a antibody (MBS178688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.lane 1: JURKAT cell lysates,lane 2: CEM whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11a antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD11a at approximately 150KD. The expected band size for CD11a is at 129KD. )
CD11a, Polyclonal Antibody (Cat# AAA178688)
Full Name
Anti-CD11a Antibody
Gene Names
ITGAL; CD11A; LFA-1; LFA1A
Reactivity
Human
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of OAT3/Slc22a8 using anti-OAT3/Slc22a8 antibody (MBS1753640).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-OAT3/Slc22a8 antigen affinity purified polyclonal antibody (Catalog # MBS1753640) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for OAT3/Slc22a8 at approximately 60KD. The expected band size for OAT3/Slc22a8 is at 60KD. )
OAT3/Slc22a8, Polyclonal Antibody (Cat# AAA1753640)
Full Name
Anti-OAT3/Slc22a8 Antibody
Gene Names
Slc22a8; Oat3; Roct
Reactivity
Mouse
Applications
WB, FC, EIA
Purity
Immunogen affinity purified.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of YAC-1 cells using anti-Mouse CD90/Thy1 antibody.
Overlay histogram showing YAC-1 cells stained with(Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mouse CD90/Thy1 Antibody (1ug/1x106 cells) for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
CD90/Thy1, Polyclonal Antibody (Cat# AAA1751387)
Full Name
Anti-Mouse CD90/Thy1 DyLight 488 conjugated Antibody
Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Reactivity
Mouse
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808378)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: ATTO 488
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,385/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of OB Cadherin/CDH11 using anti-OB Cadherin/CDH11 antibody (MBS1753660).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human PC-3 whole cell lysatesLane 2: rat brain tissue lysatesLane 3: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-OB Cadherin/CDH11 antigen affinity purified polyclonal antibody (Catalog # MBS1753660) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for OB Cadherin/CDH11 at approximately 120KD. The expected band size for OB Cadherin/CDH11 is at 120KD. )
OB Cadherin/CDH11, Polyclonal Antibody (Cat# AAA1753660)
Full Name
Anti-OB Cadherin/CDH11 Antibody
Gene Names
CDH11; OB; CAD11; CDHOB; OSF-4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of HIF-1 alpha/HIF1A using anti-HIF-1 alpha/HIF1A antibody (MBS1753259).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human A431 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: rat PC-12 whole cell lysatesLane 5: mouse thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-HIF-1 alpha/HIF1A antigen affinity purified polyclonal antibody (Catalog # MBS1753259) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for HIF-1 alpha/HIF1A at approximately 93KD. The expected band size for HIF-1 alpha/HIF1A is at 93KD. )
HIF-1 alpha/HIF1A, Polyclonal Antibody (Cat# AAA1753259)
Full Name
Anti-HIF-1 alpha/HIF1A Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG, Secondary Antibody (Cat# AAA679317)
Full Name
Goat Anti-Donkey IgG (H+L)-FITC
Reactivity
Donkey; May react with immunoglobulins from other species and the light chains of other equine immunoglobulins
Applications
EIA
Purity
Affinity chromatography on donkey IgG covalently linked to agarose
Pricing
$160/1 mg | $570/5x1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing huuman peripheral blood lymphocytes stained with CD11a antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CD11a, Monoclonal Antibody (Cat# AAA9231267)
Full Name
Human CD11a Antibody
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of BAK in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
BAK, Monoclonal Antibody (Cat# AAA200486)
Full Name
BAK
Gene Names
BAK1; BAK; CDN1; BCL2L7; BAK-LIKE
Reactivity
Human
Applications
EIA, WB, ICC, IF, FC/FACS
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808388)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: FITC
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$570/0.1 mg | $2,340/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22349a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22349a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
SHC2, Polyclonal Antibody (Cat# AAA9231297)
Full Name
SHC2 Antibody (N-Term)
Gene Names
SHC2; SCK; SLI; SHCB
Reactivity
Human
Predicted: Human
Predicted: Human
Applications
IF, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22336a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22336a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
GCA, Polyclonal Antibody (Cat# AAA9231283)
Full Name
GCA Antibody (N-Term)
Gene Names
GCA; GCL
Reactivity
Human
Applications
IF, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
IgG, Secondary Antibody (Cat# AAA679295)
Full Name
Goat Anti-Human IgG (H+L)-BIOT
Reactivity
Human; May react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
$150/1 mg | $535/5x1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of A549 cells using anti-Human ADO antibody.
Overlay histogram showing A549 cells stained with A02700-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human ADO Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
ADO, Polyclonal Antibody (Cat# AAA1751433)
Full Name
Anti-Human ADO DyLight 488 conjugated Antibody
Gene Names
ADO; C10orf22
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Testing Data
(Alexa flour 647 conjugated Mouse anti Rat CD63 and FITC conjugated Mouse IgG1 isotype control . Figure B. Alexa Fluor 647 conjugated Mouse anti Rat CD63 and FITC conjugated Mouse anti Rat CD11b . All experiments performed on rat peritoneal macrophages gated on live, single cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer)
IgG2b, Negative Control (Cat# AAA225868)
Full Name
Mouse IgG2b Negative Control: Amethyst Orange
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Pricing
$385/0.1 mg | $1,575/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing HeLa cells stained with AP22300a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22300a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
ATP1A4, Polyclonal Antibody (Cat# AAA9231236)
Full Name
ATP1A4 Antibody (N-Term)
Gene Names
ATP1A4; ATP1A1; ATP1AL2
Reactivity
Human
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808387)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: Biotin
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$575/0.1 mg | $2,365/5x0.1 mg
Testing Data
(Figure A. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2b isotype control . Figure B. Purified mouse anti bovine CD13 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD11b . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)
CD13, Monoclonal Antibody (Cat# AAA225656)
Full Name
Mouse Anti Bovine CD13
Gene Names
ANPEP; APN; CD13; P150
Reactivity
Bovine
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of HEPG2 cells using anti-Human Apolipoprotein A I antibody.
Overlay histogram showing HEPG2 cells stained with A00717-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Apolipoprotein A I Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
Apolipoprotein A I, Polyclonal Antibody (Cat# AAA1751269)
Full Name
Anti-Human Apolipoprotein A I DyLight 488 conjugated Antibody
Gene Names
APOA1; apo(a)
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of K562 cells using anti-Human Bid antibody.
Overlay histogram showing K562 cells stained with A00730-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bid Antibody for 30 min at 20 degree C. DyLight® 488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
)
Bid, Polyclonal Antibody (Cat# AAA1751271)
Full Name
Anti-Human Bid DyLight 488 conjugated Antibody
Gene Names
BID; FP497
Reactivity
Human
Applications
FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$400/0.05 mg | $1,745/5x0.05 mg
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808377)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: ATTO 390
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$585/0.1 mg | $2,385/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22347c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22347c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
GPX7, Polyclonal Antibody (Cat# AAA9231295)
Full Name
GPX7 Antibody (Center)
Gene Names
GPX7; GPX6; CL683; GPx-7; NPGPx; GSHPx-7
Reactivity
Human
Predicted: Bovine
Predicted: Bovine
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Flow Cytometry (FC/FACS)
(Figure 1. Flow Cytometry analysis of NEURO-2A cells using anti-CASR antibody (M00574).Overlay histogram showing NEURO-2A cells stained with M00574 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CASR Antibody (M00574, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CASR, Monoclonal Antibody (Cat# AAA1751988)
Full Name
Anti-CASR Antibody (monoclonal, 11E9)
Gene Names
CASR; CAR; FHH; FIH; HHC; EIG8; HHC1; NSHPT; PCAR1; hCasR; GPRC2A; HYPOC1
Reactivity
Human
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD5 using anti-CD5 antibody (MBS1750420).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,Lane 2: mouse HEPA1-6 cell lysates,Lane 3: human Hela cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD5 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD5 at approximately 67KD. The expected band size for CD5 is at 54KD. )
CD5, Polyclonal Antibody (Cat# AAA1750420)
Full Name
Anti-CD5 Picoband antibody
Gene Names
CD5; T1; LEU1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Presenilin 2/AD5/PSEN2 using anti-Presenilin 2/AD5/PSEN2 antibody (MBS1753370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human placenta tissue lysatesLane 2: human HEK293 whole cell lysatesLane 3: human CACO-2 whole cell lysatesLane 4: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Presenilin 2/AD5/PSEN2 antigen affinity purified polyclonal antibody (Catalog # MBS1753370) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Presenilin 2/AD5/PSEN2 at approximately 34KD,50KD. The expected band size for Presenilin 2/AD5/PSEN2 is at 50KD. )
Presenilin 2/AD5/PSEN2, Polyclonal Antibody (Cat# AAA1753370)
Full Name
Anti-Presenilin 2/AD5/PSEN2 Antibody
Gene Names
PSEN2; AD4; PS2; AD3L; STM2; CMD1V
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
IgG Fd, Secondary Antibody (Cat# AAA679298)
Full Name
Goat Anti-Human IgG Fd-FITC
Reactivity
Human IgG Fc, IgM, and IgA; may react with IgG from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on human IgG covalently linked to agarose
Pricing
$245/0.5 mg | $940/5x0.5 mg
Immunofluroescence (IF)
(Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (MBS150780))
COVID 19 Spike Coronavirus, Polyclonal Antibody (Cat# AAA154771)
Full Name
SARS-CoV-2 (COVID-19) Spike Antibody (biotin)
Reactivity
Virus
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (100%)
Predicted reactivity based on immunogen sequence: SARS-CoV Spike proteins: (100%)
Purity
SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.
Pricing
$495/0.1 mg | $2,225/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of LSM7 using anti-LSM7 antibody (MBS1753799).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human U937 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human MCF-7 whole cell lysatesLane 6: human Jurkat whole cell lysatesLane 7: human Raji whole cell lysatesLane 8: rat brain tissue lysatesLane 9: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-LSM7 antigen affinity purified polyclonal antibody (Catalog # MBS1753799) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for LSM7 at approximately 12KD. The expected band size for LSM7 is at 12KD. )
LSM7, Polyclonal Antibody (Cat# AAA1753799)
Full Name
Anti-LSM7 Antibody
Gene Names
LSM7; YNL147W
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of CLEC4E in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray))
CLEC4E, Monoclonal Antibody (Cat# AAA200500)
Full Name
CLEC4E
Gene Names
CLEC4E; MINCLE; CLECSF9
Reactivity
Human
Applications
EIA, WB, ICC, IF, FC/FACS
Purity
By protein-A affinity chromatography
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of MBS626297 in mouse stomach tissue lysates (35ug/lane). PARD3 (arrow) was detected using the purified Pab.)
ASIP, Polyclonal Antibody (Cat# AAA6464263)
Full Name
ASIP, CT (Atypical PKC Isotype-specific-interacting Protein, Bazooka, Baz, CTCL Tumor Antigen se2-5, FLJ21015, Partitioning Defective 3 Homolog, PAR3, PAR-3, PARD3, PARD-3, PAR3alpha, PAR3-alpha, PAR3A, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2) (PE)
Gene Names
PARD3; Baz; ASIP; PAR3; PARD-3; PARD3A; SE2-5T2; PPP1R118; SE2-5L16; SE2-5LT1; PAR3alpha
Reactivity
Human, Mouse
Applications
WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of AHR using anti-AHR antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human AHR protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AHR antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AHR at approximately 36KD. The expected band size for AHR is at 36KD.)
AHR, Polyclonal Antibody (Cat# AAA1751223)
Full Name
Anti-AHR Antibody
Gene Names
AHR; bHLHe76
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(ICC/IF analysis of IRF-5 in THP-1 cells. The cell was stained at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray). )
IRF-5, Monoclonal Antibody (Cat# AAA200481)
Full Name
IRF-5
Gene Names
IRF5; SLEB10
Reactivity
Human
Applications
EIA, WB, ICC, IF, FC/FACS
Pricing
$275/0.05 mL | $360/0.1 mL | $1,375/5x0.1 mL
