Western Blot (WB)
(All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse CLCN1 Polyclonal Antibody | anti-CLCN1 antibody
CLCN1 Antibody (N-Term)
WB: 1:2000
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-CLCN1 Antibody (N-Term) at 1:2000 dilutionLane 1: Human skeletal muslce lysateLane 2: Mouse skeletal muscle lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 109 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Testing Data (TD)
(Overlay histogram showing Hela cellsstained with (MBS9231248)(green line). The cellswere fixed with 2% paraformaldehyde (10min) and then permeabilized with 90%methanol for 10 min. The cells were thenicubated in 2% bovine serum albumin to block non-specific protein-protein interactionsfollowed by the antibody (MBS9231248, 1:25dilution) for 60 min at 37ºC. The secondaryantibody used was Goat-Anti-Rabbit IgG,DyLight® 488 Conjugated HighlyCross-Adsorbed(1583138) at 1/200 dilutionfor 40 min at 37ºC. Isotype control antibody(blue line) was rabbit IgG1 (1?g/1x10^6cells) used under the same conditions.Acquisition of >10, 000 events wasperformed.)
Testing Data (TD)
(Overlay histogram showing Hela cellsstained with (MBS9231248)(green line). The cellswere fixed with 2% paraformaldehyde (10min) and then permeabilized with 90%methanol for 10 min. The cells were thenicubated in 2% bovine serum albumin to block non-specific protein-protein interactionsfollowed by the antibody (MBS9231248, 1:25dilution) for 60 min at 37ºC. The secondaryantibody used was Goat-Anti-Rabbit IgG,DyLight® 488 Conjugated HighlyCross-Adsorbed(1583138) at 1/200 dilutionfor 40 min at 37ºC. Isotype control antibody(blue line) was rabbit IgG1 (1?g/1x10^6cells) used under the same conditions.Acquisition of >10, 000 events wasperformed.)
NCBI and Uniprot Product Information
NCBI Description
The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. The protein encoded by this gene regulates the electric excitability of the skeletal muscle membrane. Mutations in this gene cause two forms of inherited human muscle disorders: recessive generalized myotonia congenita (Becker) and dominant myotonia (Thomsen). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2012]
Uniprot Description
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport.
Research Articles on CLCN1
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Product Notes
The CLCN1 clcn1 (Catalog #AAA9231248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLCN1 Antibody (N-Term) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLCN1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC), Western Blot (WB), ELISA (EIA). FC: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the CLCN1 clcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLCN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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