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Flow Cytometry (FC/FACS) (Overlay histogram showing huuman peripheral blood monocytes stained with CD14 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Mouse anti-Human CD14 Monoclonal Antibody | anti-CD14 antibody

Human CD14 Antibody

Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Synonyms
CD14; Monoclonal Antibody; Human CD14 Antibody; Purified Mouse Monoclonal Antibody (Mab); anti-CD14 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3C10
Purity/Purification
Purified through a protein G column, followed by dialysis against PBS.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
373
Applicable Applications for anti-CD14 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
FC/FACS: 1:50
Antigen Type
Recombinant Protein
Antigen Source
Human
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Overlay histogram showing huuman peripheral blood monocytes stained with CD14 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

Flow Cytometry (FC/FACS) (Overlay histogram showing huuman peripheral blood monocytes stained with CD14 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Product Categories/Family for anti-CD14 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
39,667 Da
NCBI Official Full Name
CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
UniProt Gene Name
CD14

Uniprot Description

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().

Research Articles on CD14

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Product Notes

The CD14 cd14 (Catalog #AAA9231265) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human CD14 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). FC/FACS: 1:50. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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