Flow Cytometry (FC/FACS)
(Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit CKS2 Polyclonal Antibody | anti-CKS2 antibody
CKS2 Antibody (N-Term)
Predicted: Bovine, Mouse
Predicted: Bovine, Mouse
WB: 1:2000
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot (WB)
(All lanes : Anti-CKS2 Antibody (N-Term) at 1:2000 dilutionLane 1: Caco2 whole cell lysateLane 2: RPMI 8226 whole cell lysateLane 3: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-CKS2 Antibody (N-Term) at 1:2000 dilutionLane 1: Caco2 whole cell lysateLane 2: RPMI 8226 whole cell lysateLane 3: SH-SY5Y whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
CKS2 protein binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. The CKS2 mRNA is found to be expressed in different patterns through the cell cycle in HeLa cells, which reflects specialized role for the encoded protein. [provided by RefSeq, Jul 2008]
Uniprot Description
Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function.
Research Articles on CKS2
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Product Notes
The CKS2 cks2 (Catalog #AAA9231257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CKS2 Antibody (N-Term) reacts with Human Predicted: Bovine, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CKS2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). FC/FACS: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the CKS2 cks2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CKS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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