Western Blot (WB)
(Figure 1. Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: rat spleen tissue lysatesLane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Carbonic Anhydrase I/CA1 antigen affinity purified monoclonal antibody (Catalog # MBS1753963) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 29KD. The expected band size for Carbonic Anhydrase I/CA1 is at 29KD. )
Mouse Carbonic Anhydrase I/CA1 Monoclonal Antibody | anti-CA1 antibody
Anti-Carbonic Anhydrase I/CA1 Antibody (monoclonal, 4D5)
Concentration: 0.25-0.5ug/ml
Tested Species: Human, Mouse, Rat
IHC-P:
Concentration: 2-5ug/ml
Tested Species: Human
FC:
Concentration: 1-3ug/1x106 cells
Tested Species: Human
Lab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (MBS176445) for Western Blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (MBS176472 ) for IHC(P).
Western Blot (WB)
(Figure 1. Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: rat spleen tissue lysatesLane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Carbonic Anhydrase I/CA1 antigen affinity purified monoclonal antibody (Catalog # MBS1753963) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 29KD. The expected band size for Carbonic Anhydrase I/CA1 is at 29KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: rat spleen tissue lysatesLane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Carbonic Anhydrase I/CA1 antigen affinity purified monoclonal antibody (Catalog # MBS1753963) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176445) with Tanon 5200 system. A specific band was detected for Carbonic Anhydrase I/CA1 at approximately 29KD. The expected band size for Carbonic Anhydrase I/CA1 is at 29KD. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 3. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 5. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 6. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 7. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 7. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 8. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Immunohistochemistry (IHC)
(Figure 8. IHC analysis of Carbonic Anhydrase I/CA1 using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Carbonic Anhydrase I/CA1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Carbonic Anhydrase I/CA1 Antibody (MBS1753963) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176438) with DAB as the chromogen. )
Flow Cytometry (FC)
(Figure 9. Flow Cytometry analysis of SiHa cells using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Overlay histogram showing SiHa cells stained with MBS1753963 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Carbonic Anhydrase I/CA1 Antibody (MBS1753963, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC)
(Figure 9. Flow Cytometry analysis of SiHa cells using anti-Carbonic Anhydrase I/CA1 antibody (MBS1753963).Overlay histogram showing SiHa cells stained with MBS1753963 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Carbonic Anhydrase I/CA1 Antibody (MBS1753963, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. "Entrez Gene: CA1 carbonic anhydrase I".
NCBI and Uniprot Product Information
NCBI Description
Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA1 is closely linked to CA2 and CA3 genes on chromosome 8, and it encodes a cytosolic protein which is found at the highest level in erythrocytes. Variants of this gene have been described in some populations. Multiple alternatively spliced variants, encoding the same protein, have been identified. Transcript variants of CA1 utilizing alternative polyA_sites have been described in literature. [provided by RefSeq, Sep 2009]
Uniprot Description
CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.
Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen
Chromosomal Location of Human Ortholog: 8q21.2
Cellular Component: cytosol
Molecular Function: carbonate dehydratase activity; zinc ion binding
Biological Process: bicarbonate transport; one-carbon compound metabolic process
Research Articles on CA1
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Product Notes
The CA1 ca1 (Catalog #AAA1753963) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Carbonic Anhydrase I/CA1 Antibody (monoclonal, 4D5) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase I/CA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC). WB: Concentration: 0.25-0.5ug/ml Tested Species: Human, Mouse, Rat IHC-P: Concentration: 2-5ug/ml Tested Species: Human FC: Concentration: 1-3ug/1x106 cells Tested Species: Human Lab recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (MBS176445) for Western Blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (MBS176472 ) for IHC(P). Researchers should empirically determine the suitability of the CA1 ca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase I/CA1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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