Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Goat IgG Fc " - showing 850-900
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TEC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Tec, Polyclonal Antibody (Cat# AAA125623)
Full Name
Tec Rabbit pab Antibody
Gene Names
TEC; PSCTK4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, IP, FC/FACS
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Pig CD3 and Alexa Fluor 647 conjugated Mouse anti Pig CD4 . All experiments performed on frozen porcine blood gated on live single cell lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
CD4, Monoclonal Antibody (Cat# AAA225685)
Full Name
Mouse Anti Pig CD4: FITC
Reactivity
Pig
Applications
FC/FACS, IF
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PIAS1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
PIAS1, Polyclonal Antibody (Cat# AAA125862)
Full Name
PIAS1
Gene Names
PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
Reactivity
Human, mouse, rat
Applications
WB, IHC, ICC, IP, FC/FACS
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Hela cells using PPARA Antibody(green) compared to an isotype control of mouse IgG1(blue). MBS9200027 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
PPARA, Monoclonal Antibody (Cat# AAA9200027)
Full Name
PPARA Antibody
Gene Names
PPARA; PPAR; NR1C1; hPPAR; PPARalpha
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IF, IHC
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108). Tissue: Cervical cancer cell line (HeLa). Species: Human. Primary Antibody: Rabbit Anti-Calnexin Polyclonal Antibody (SPC-108) at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).)
Calnexin-CT, Polyclonal Antibody (Cat# AAA805666)
Full Name
Calnexin-CT Antibody: ATTO 594
Gene Names
CANX; PP90
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
Applications
WB, IHC, ICC, IP, FC/FACS, IF
Pricing
$560/0.2 mL | $2,290/5x0.2 mL
Western Blot (WB)
(Western blot analysis of FCGR1B Antibody (C-term) in CEM cell line lysates (35ug/lane). FCGR1B (arrow) was detected using the purified Pab.)
FCGR1B, Polyclonal Antibody (Cat# AAA9202692)
Full Name
FCGR1B Antibody (C-term)
Gene Names
FCGR1B; CD64b; IGFRB
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of Rat tissue mix showing detection of Calnexin-CT protein using Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calnexin-CT Polyclonal Antibody (SPC-182) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.)
Calnexin-CT, Polyclonal Antibody (Cat# AAA806589)
Full Name
Calnexin-CT Antibody: HRP
Gene Names
CANX; PP90
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Chicken (weak), Dog, Guinea pig, Hamster, Pig, Quail, Rabbit, Sheep, Drosophila (weak), Xenopus (weak)
Applications
WB, IP, ICC, IHC, FC/FACS
Pricing
$550/0.2 mg | $2,240/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), Hela (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.)
beta-Actin, Antibody (Cat# AAA850843)
Full Name
beta-Actin Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Hamster, Rat, Monkey
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(All lanes : RAB1B Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 22 KDa Observed band size : 22 KDa Blocking/Dilution buffer : 1x TBST.)
RAB1B, Monoclonal Antibody (Cat# AAA435184)
Full Name
RAB1B Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD28 Mouse Monoclonal Antibody (C28/76) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)
CD28, Monoclonal Antibody (Cat# AAA4381419)
Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human
Applications
FC/FACS
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).)
CMG1/CCDC2/IFT74, Antibody (Cat# AAA423730)
Full Name
Goat anti-CMG1/CCDC2/IFT74 Antibody
Gene Names
IFT74; CMG1; BBS20; CCDC2; CMG-1
Reactivity
Human
Applications
EIA, IF, FC/FACS
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western blot analysis of lysate from human liver tissue lysate, using GPD1 Antibody (N-term). MBS9207360 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
GPD1, Polyclonal Antibody (Cat# AAA9207360)
Full Name
GPD1 Antibody (N-term)
Gene Names
GPD1; GPD-C; HTGTI; GPDH-C
Reactivity
Human
Applications
EIA, IHC, FC/FACS, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Anti-PKM2 Antibody (C-term L398) at 1:1000 dilution + Hela whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
PKM2, Polyclonal Antibody (Cat# AAA9217135)
Full Name
PKM2 Antibody (C-term L398)
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
CDC42, Monoclonal Antibody (Cat# AAA435096)
Full Name
CDC42 Mouse mAb
Gene Names
CDC42; TKS; G25K; CDC42Hs
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data #1
(Flow cytometric detection of CD1c staining on bovine peripheral blood monocytes using Goat anti Mouse IgG:FITC (MBS235149) to bind Mouse anti Bovine CD1c (MBS224734))
IgG, Polyclonal Antibody (Cat# AAA235149)
Full Name
GOAT ANTI MOUSE IgG:FITC (RAT ADSORBED)
Applications
FC/FACS
Pricing
$735/0.5 mg | $3,155/5x0.5 mg
Western Blot (WB)
(All lanes : K2C6B Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.)
KRT6B/K2C6B, Monoclonal Antibody (Cat# AAA435143)
Full Name
KRT6B/K2C6B Mouse mAb
Gene Names
KRT6B; K6B; PC2; PC4; CK6B; CK-6B; KRTL1
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from A431,Hela,Jurkat cell line (from left to right), using BAT1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
BAT1, Polyclonal Antibody (Cat# AAA9231816)
Full Name
BAT1 Antibody (C-term)
Gene Names
DDX39B; BAT1; UAP56; D6S81E
Reactivity
Human
Predicted: Mouse, Rat, Bovine, Chicken, Drosophila, Pig
Predicted: Mouse, Rat, Bovine, Chicken, Drosophila, Pig
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))
CD204, Monoclonal Antibody (Cat# AAA214931)
Full Name
RAT ANTI MOUSE CD204
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Reactivity
Channel catfish, Pig
Applications
EIA, FC/FACS, IP, WB
Pricing
$475/0.25 mg | $1,995/5x0.25 mg
Testing Data
(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))
CD3, Monoclonal Antibody (Cat# AAA216373)
Full Name
MOUSE ANTI HUMAN CD3:FITC
Gene Names
CD3G; T3G; IMD17; CD3-GAMMA
Applications
FC/FACS
Pricing
$195/0.025 mg | $730/5x0.025 mg
Western Blot (WB)
(Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein CD3E (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
CD3E, Monoclonal Antibody (Cat# AAA200286)
Full Name
CD3E antibody
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from MCF-7,Jurkat,PC-12 cell line (from left to right), using TOP1 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
TOP1, Monoclonal Antibody (Cat# AAA9231643)
Full Name
TOP1 Antibody (N-term)
Gene Names
TOP1; TOPI
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
WB, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-DDX39 monoclonal antibody. )
DDX39, Monoclonal Antibody (Cat# AAA120168)
Full Name
Mouse monoclonal antibody Anti-Human DDX39
Gene Names
DDX39A; BAT1; DDXL; BAT1L; DDX39; URH49
Reactivity
Human
Applications
WB
Western Blot (WB)
(Western blot analysis of CFL1 Antibody (Center) (MBS9205122) in HL-60 cell line lysates (35ug/lane). CFL1 (arrow) was detected using the purified Pab;)
CFL1, Polyclonal Antibody (Cat# AAA9205122)
Full Name
CFL1 Antibody (Center)
Gene Names
CFL1; CFL; cofilin; HEL-S-15
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Monkey, Pig, Rat, Chicken, Xenopus)
Applications
WB, EIA, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
IgG (H+L), Secondary Antibody (Cat# AAA128206)
Full Name
Cy3-Rabbit Anti-Goat IgG (H+L) Antibody
Applications
ICC, FC/FACS
Purity
Affinity Purification
Pricing
$130/0.1 mL | $440/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using MBS768438 (KRT18 Antibody) at dilution of 1:200)
Cytokeratin 18, Monoclonal Antibody (Cat# AAA768438)
Full Name
Cytokeratin 18 Mouse Monoclonal
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Applications
EIA, WB, FC/FACS, IF, IHC
Purity
>=95% as determined by SDS-PAGE
Protein A+G purification
Protein A+G purification
Pricing
$365/0.1 mg | $1,530/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GRIA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
GLUR4, Polyclonal Antibody (Cat# AAA127641)
Full Name
GLUR4 Polyclonal Antibody
Gene Names
GRIA4; GLUR4; GLURD; GluA4; GLUR4C
Reactivity
Human, Mouse, Rat
Applications
WB, FC/FACS
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 .)
CD5, Monoclonal Antibody (Cat# AAA225718)
Full Name
Mouse Anti Bovine CD5
Reactivity
Bovine, Goat, Sheep
Applications
IHC, FC/FACS, IP
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Western Blot (WB)
HNRPL, Monoclonal Antibody (Cat# AAA435092)
Full Name
HNRPL Mouse mAb
Gene Names
HNRNPL; HNRPL; hnRNP-L; P/OKcl.14
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data
(Figure A. RPE conjugated Rat anti Mouse CD3 and FITC conjugated Rat IgG2b isotype control . Figure B. RPE conjugated Rat anti Mouse CD3 and FITC conjugated Rat anti Mouse CD8 . All experiments performed on red cell lysed mouse blood gated on lymphocytes in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)
CD8, Monoclonal Antibody (Cat# AAA225633)
Full Name
Rat Anti Mouse CD8: FITC
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$415/0.1 mg | $1,710/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from Jurkat,K562 cell line (from left to right), using BRAF Antibody. It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.)
BRAF, Polyclonal Antibody (Cat# AAA9231896)
Full Name
BRAF Antibody
Gene Names
BRAF; NS7; B-raf; BRAF1; RAFB1; B-RAF1
Reactivity
Human
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Testing Data #1
(Staining of equine peripheral blood lymphocytes with Mouse anti Equine CD44 (MBS224573), followed by Goat anti Mouse IgG:FITC (MBS235142))
CD44, Monoclonal Antibody (Cat# AAA224456)
Full Name
MOUSE ANTI HORSE CD44:RPE
Applications
FC/FACS
Pricing
$640/100 Tests | $2,735/5x100 Tests
Testing Data
(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 (MBS216055) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195) Low power)
CD13, Monoclonal Antibody (Cat# AAA216058)
Full Name
RAT ANTI MOUSE CD13
Gene Names
Anpep; Apn; AP-M; AP-N; Cd13; P150
Applications
FC/FACS
Pricing
$210/0.025 mg | $800/5x0.025 mg
Western Blot (WB)
(Western blot analysis of lysates from huamn liver tissue,Jurkat cell line (from left to right), using GSTM1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
GSTM1, Polyclonal Antibody (Cat# AAA9231743)
Full Name
GSTM1 Antibody (C-term)
Gene Names
GSTM1; MU; H-B; GST1; GTH4; GTM1; MU-1; GSTM1-1; GSTM1a-1a; GSTM1b-1b
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from K562,PC-3,Y79 cell line (from left to right), using GSTP1 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
GSTP1, Polyclonal Antibody (Cat# AAA9231832)
Full Name
GSTP1 Antibody (C-term)
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human, Rat
Applications
WB, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from NCI-H1299, Raji cell line (from left to right), using HLA-DRB1 Antibody (N-term). MBS9202697 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
HLA-DRB1, Polyclonal Antibody (Cat# AAA9202697)
Full Name
HLA-DRB1 Antibody (N-term)
Reactivity
Human
Applications
FC/FACS, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))
CD204, Monoclonal Antibody (Cat# AAA210655)
Full Name
RAT ANTI MOUSE CD204:FITC
Gene Names
Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
Applications
FC/FACS
Pricing
$260/0.05 mg | $1,020/5x0.05 mg
Western Blot (WB)
(All lanes : Anti-DFFB Antibody (N-term) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: HepG2 whole cell lysatesLane 3: Jurkat whole cell lysatesLane 4: LNCaP whole cell lysatesLane 5: Raji whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 39 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
DFFB, Polyclonal Antibody (Cat# AAA9213078)
Full Name
DFFB Antibody (N-term)
Gene Names
DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)
Hsp60, Monoclonal Antibody (Cat# AAA802607)
Full Name
Hsp60 Antibody: ATTO 594
Gene Names
HSPD1; HLD4; CPN60; GROEL; HSP60; HSP65; SPG13; HSP-60; HuCHA60
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Spinach, E.coli (GroEl), H. pylori, S. typhimurium, T. spiralis, Yeast, White Fly
Applications
WB, IHC, FC/FACS
Pricing
$500/0.2 mg | $2,020/5x0.2 mg
Western Blot (WB)
(Western blot analysis of FCGR2B using Jurkat whole lysates.)
FCGR2B, Polyclonal Antibody (Cat# AAA9608250)
Full Name
FCGR2B Antibody
Gene Names
FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2; FCGR2C; FcRII-c
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of lysate from 293 cell line, using PLA2G4A Antibody (Center). MBS9200883 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
PLA2G4A, Polyclonal Antibody (Cat# AAA9200883)
Full Name
PLA2G4A Antibody (Center)
Gene Names
PLA2G4A; PLA2G4; cPLA2-alpha
Reactivity
Human (Predicted Reactivity: Chicken, Zebrafish, Mouse, Rabbit, Rat)
Applications
EIA, IHC, IF, WB, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Nitrotryptophan, Monoclonal Antibody (Cat# AAA808828)
Full Name
Nitrotryptophan Antibody, Clone 2D12: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808664)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5E8
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808765)
Full Name
Malondialdehyde Antibody, Clone 6H6: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Testing Data
(Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC (MBS216414))
F4/80, Monoclonal Antibody (Cat# AAA216428)
Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
EM, FC/FACS, IF, IP, RIA, RE, WB
Pricing
$210/0.025 mg | $800/5x0.025 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215789)
Full Name
RAT ANTI MOUSE DECTIN-1
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS, IP
Pricing
$525/0.25 mg | $2,210/5x0.25 mg
Western Blot (WB)
(Anti-ST3GAL5 Antibody (C-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 48 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)
ST3GAL5, Polyclonal Antibody (Cat# AAA9201241)
Full Name
ST3GAL5 Antibody (C-term)
Gene Names
ST3GAL5; SATI; SIAT9; ST3GalV; SIATGM3S
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
IgG (H+L), Secondary Antibody (Cat# AAA128188)
Full Name
FITC Goat Anti-Mouse IgG (H+L) Antibody
Applications
ICC, FC/FACS
Purity
Affinity Purification
Pricing
$120/0.1 mL | $395/5x0.1 mL
Testing Data
(Staining of horse peripheral blood lymphocytes with Mouse anti Horse Pan B-Cells followed by Goat anti Mouse IgG:FITC (MBS235142))
PAN B-CELLS, Monoclonal Antibody (Cat# AAA219907)
Full Name
MOUSE ANTI HORSE PAN B-CELLS
Applications
EIA, FC/FACS
Pricing
$365/0.1 mg | $1,495/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of SKOV3 cells, using PAX8 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
PAX8, Polyclonal Antibody (Cat# AAA125503)
Full Name
PAX8 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IP, FC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using FCGR2B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
FCGR2B, Polyclonal Antibody (Cat# AAA9132446)
Full Name
FCGR2B Polyclonal Antibody
Gene Names
FCGR2B; CD32; FCG2; CD32B; FCGR2; IGFR2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
