Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GRIA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Rabbit GLUR4 Polyclonal Antibody | anti-GRIA4 antibody
GLUR4 Polyclonal Antibody
Gene Names
GRIA4; GLUR4; GLURD; GluA4; GLUR4C
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
GLUR4; Polyclonal Antibody; GLUR4 Polyclonal Antibody; GRIA4 ; GLURD; GluA4; GLUR4C; anti-GRIA4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
902
Applicable Applications for anti-GRIA4 antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1:500-1:2000
Flow Cytometry (FC/FACS): 1:20-1:100
Flow Cytometry (FC/FACS): 1:20-1:100
Immunogen
A synthetic peptide of human GLUR4
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GRIA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using GRIA4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
Related Product Information for anti-GRIA4 antibody
AMPA(alphaamino3hydroxy5methyl4isoxazolepropionic acid), kainite, and NMDA(NmethylDaspartate) receptors are the three main families of ionotropic glutamategated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 14), which assemble as homoor heterotetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2containing AMPARs (2). Posttranscriptional modifications (alternative splicing, nuclear RNA editing) and posttranslational modifications (glycosylation, phosphorylation) result in a very large number of permutations, finetuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer's, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1). GluR 4 containing AMPA receptors are found in synapses and GluR 4 delivery to synapses and cell surface expression is mediated through phosphorylation of Ser842 by PKA or PKC (3).
References
1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 25377. 2. CullCandy, S. et al. (2006) Curr Opin Neurobiol 16, 28897. 3. Gomes, A.R. et al. (2007) Traffic 8, 259269.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101kDa
NCBI Official Full Name
glutamate receptor 4 isoform 1
NCBI Official Synonym Full Names
glutamate receptor, ionotropic, AMPA 4
NCBI Official Symbol
GRIA4
NCBI Official Synonym Symbols
GLUR4; GLURD; GluA4; GLUR4C
NCBI Protein Information
glutamate receptor 4; gluR-4; gluR-D; AMPA-selective glutamate receptor 4; glutamate receptor, ionotrophic, AMPA 4
UniProt Protein Name
Glutamate receptor 4
Protein Family
UniProt Gene Name
GRIA4
UniProt Synonym Gene Names
GLUR4; GluR-4; GluR4; GluA4
UniProt Entry Name
GRIA4_HUMAN
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Product Notes
The GRIA4 gria4 (Catalog #AAA127641) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GLUR4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GLUR4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). Western Blot (WB): 1:500-1:2000 Flow Cytometry (FC/FACS): 1:20-1:100. Researchers should empirically determine the suitability of the GRIA4 gria4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLUR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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