Mouse CD5 Monoclonal Antibody | anti-CD5 antibody

Mouse Anti Bovine CD5

Cat. Num. AAA225718

• Reactivity: Bovine, Goat, Sheep
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Synonyms: CD5; Monoclonal Antibody; Mouse Anti Bovine CD5; CD5 antibody (CC17); anti-CD5 antibody

• Host: Mouse
• Reactivity: Bovine, Goat, Sheep
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: CC17
• Purity/Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Form/Format: Liquid; Phosphate Buffered Saline
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 495

• Applicable Applications for anti-CD5 antibody: Immunohistochemistry (IHC) Frozen, Flow Cytometry (FC/FACS), Immunoprecipitation (IP)
• Immunogen: Bovine thymocytes
• Carrier Free: Yes
• Fusion Partners: Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.
• Preservative Stabilizers: 0.09% Sodium Azide (NaN3)
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred.; This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.; Should this product contain a precipitate we recommend microcentrifugation before use.

Testing Data

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 .)

Testing Data

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD5 followed by Goat anti Mouse IgG (H/L):FITC .)

Testing Data

(Figure A. Purified mouse anti bovine CD5 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse IgG2a isotype control . Figure B. Purified mouse anti bovine CD5 detected with goat anti mouse IgG1 RPE and FITC conjugated mouse anti bovine CD4 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Testing Data

(Figure A. FITC conjugated mouse anti bovine CD4 and purified mouse IgG1 isotype control detected with RPE conjugated goat anti mouse IgG1 . Figure B. FITC conjugated mouse anti bovine CD4 and purified CD5 detected with RPE conjugated goat anti mouse IgG1 . All experiments performed on red cell lysed bovine blood gated on mononuclear cells.)

Related Product Information for anti-CD5 antibody

Mouse anti Bovine CD5 antibody, clone CC15 recognizes bovine CD5, a ~67 kDa type 1 single pass transmembrane molecule containing three scavenger receptor cysteine rich (SRCR) domains. Clone CC17 reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos taurus animals and a small proportion of Bos indicus animals. Bo5.1, recognized by Mouse anti Bovine CD5, clone CC17 is the only isoform expressed by Bos taurus while Bos indicus may express Bo5.2 or both allelic forms of bovine CD5. (Howard et al.1989).
Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes (Howard et al. 1988).

NCBI and Uniprot Product Information

• NCBI GI #: 27806917
• NCBI GeneID: 280745
• NCBI Accession #: NP_776324.1
• NCBI GenBank Nucleotide #: NM_173899.2
• UniProt Accession #: P19238
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: CD5 molecule
• NCBI Official Symbol: CD5
• NCBI Protein Information: T-cell surface glycoprotein CD5
• UniProt Protein Name: T-cell surface glycoprotein CD5
• Protein Family: CD5 antigen
• UniProt Gene Name: CD5
• UniProt Entry Name: CD5_BOVIN