Rat CD204 Monoclonal Antibody | anti-CD204 antibody

RAT ANTI MOUSE CD204

Cat. Num. AAA214931

• Gene Names: Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
• Reactivity: Channel catfish, Pig
• Applications: Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CD204; Monoclonal Antibody; RAT ANTI MOUSE CD204; anti-CD204 antibody

• Host: Rat
• Reactivity: Channel catfish, Pig
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 2F8
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-CD204 antibody: Immunohistology Frozen*, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)*
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.; Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Western Blotting: Application Note: This product recognises CD204 in J774 cells under non-reduced conditions only.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Raw 264 cell line; Fusion Partners
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC (MBS214937))

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647 (MBS214931A647))

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). High power)

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin (MBS214934))

Testing Data

(Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)

Testing Data

(Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC (MBS210655))

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). Medium power)

Testing Data

(Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody (MBS235195). Low power)

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin (MBS214936))

Testing Data

(Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488 (MBS214931A488))

NCBI and Uniprot Product Information

• NCBI GI #: 164664520
• NCBI GeneID: 20288
• NCBI Accession #: NP_001106797.1
• NCBI GenBank Nucleotide #: NM_001113326.1
• Molecular Weight: 38,773 Da
• NCBI Official Full Name: macrophage scavenger receptor types I and II isoform b
• NCBI Official Synonym Full Names: macrophage scavenger receptor 1
• NCBI Official Symbol: Msr1
• NCBI Official Synonym Symbols: MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1
• NCBI Protein Information: macrophage scavenger receptor types I and II; SR-A; macrophage acetylated LDL receptor I and II; scavenger receptor type A
• UniProt Protein Name: Macrophage scavenger receptor types I and II
• UniProt Gene Name: Msr1
• UniProt Synonym Gene Names: Scvr; SR-A
• UniProt Entry Name: MSRE_MOUSE

Uniprot Description

MSR1: Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. Defects in MSR1 may be a cause of prostate cancer (PC). A malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet- ring cell carcinoma and neuroendocrine carcinoma. MSR1 variants may play a role in susceptibility to prostate cancer. MSR1 variants have been found in individuals with prostate cancer and co-segregate with the disease in some families. Defects in MSR1 may be a cause of Barrett esophagus (BE). A condition characterized by a metaplastic change in which normal esophageal squamous epithelium is replaced by a columnar and intestinal-type epithelium. Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes. Genetic variants in MSR1 have been found in individuals with Barrett esophagus and are thought to contribute to disease susceptibility. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: collagen; membrane; plasma membrane; integral to membrane; cytoplasmic vesicle; cytosol

Molecular Function: low-density lipoprotein binding; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; cholesterol transport; lipoprotein transport; endocytosis