Western Blot (WB)
(Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit IRGC Polyclonal Antibody | anti-IRGC antibody
IRGC Antibody
Gene Names
IRGC; IFGGE; IRGC1; Iigp5; CINEMA; R30953_1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
IRGC Antibody is affinity chromatography purified via peptide column.
Synonyms
IRGC; Polyclonal Antibody; IRGC Antibody; IFGGE; IRGC1; Iigp5; CINEMA; R30953_1; IIGP5; Interferon-inducible GTPase 5; Immunity-related GTPase cinema 1; immunity-related GTPase family; cinema; anti-IRGC antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Two isoforms of IRGC are known to exist; this antibody will recognize both isoforms.
Purity/Purification
IRGC Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
463
Applicable Applications for anti-IRGC antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
IRGC antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human IRGC.
Buffer
IRGC Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
IRGC antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of IRGC in mouse brain tissue lysate with IRGC antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of IRGC in A20 cells with IRGC antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of IRGCb in A20 cells with IRGCb antibody at 20 μg/mL.)
Related Product Information for anti-IRGC antibody
IRGC Antibody: Immunity-related GTPases (IRG) (also known as p47 GTPases) are a family of GTPase proteins found in vertebrates, which play critical roles in mediating innate resistance to intracellular pathogens. IRG genes have been found in a number of mammals and lower species including mice, rats, zebrafish and humans. Most of the mouse genes contain interferon-stimulated response elements which mediate transcriptional activation by IFNs. In humans, only two IRG genes have been found: human IRGC encodes a full-length IRG protein that, like the mouse homologue, is constitutively expressed in testis, while human IRGM encodes a considerably truncated protein that is constitutively expressed in cultured cells including some macrophage cell lines. As the two human genes IRGC and IRGM are not subject to IFN control, it has been suggested that the host resistance mechanism supported by IRG proteins in the mouse is lacking in humans.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,288 Da
NCBI Official Full Name
interferon-inducible GTPase 5
NCBI Official Synonym Full Names
immunity-related GTPase family, cinema
NCBI Official Symbol
IRGC
NCBI Official Synonym Symbols
IFGGE; IRGC1; Iigp5; CINEMA; R30953_1
NCBI Protein Information
interferon-inducible GTPase 5; interferon inducible GTPase 5; immunity-related GTPase cinema 1; immunity-related GTPase family, cinema 1; interferon-gamma-inducible GTPase IFGGE protein
UniProt Protein Name
Interferon-inducible GTPase 5
Protein Family
UniProt Gene Name
IRGC
UniProt Synonym Gene Names
IIGP5; IRGC1
UniProt Entry Name
IIGP5_HUMAN
Uniprot Description
IRGC: Belongs to the interferon-inducible GTPase family.
Protein type: EC 3.6.5.-; Hydrolase
Chromosomal Location of Human Ortholog: 19q13.31
Cellular Component: membrane
Molecular Function: GTP binding; hydrolase activity
Biological Process: metabolic process
Research Articles on IRGC
Similar Products
Product Notes
The IRGC irgc (Catalog #AAA151122) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IRGC Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IRGC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). IRGC antibody can be used for detection of IRGC by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the IRGC irgc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRGC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
