Testing Data
(Binding curve of anti-IgE antibody TES-C21 to human IgE Fc domain ELISA Plate coated with human IgE Fc domain at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
IgE recombinant antibody
Anti-IgE [TES-C21]
Reactivity
Human
Applications
ELISA
Purity
Protein A Affinity Purified
Synonyms
IgE; Monoclonal Recombinant Antibody; Anti-IgE [TES-C21]; Immunoglobulin E; IgE recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
TES-C21
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Sequence Length
569
Applicable Applications for IgE recombinant antibody
Blocking (BL), ELISA (EIA)
Antibody Type
Recombinant Antibody
Antibody Species
Mouse
Original Species Name
Mouse
Immunogen
Polyclonal human IgE.
Chimeric Use Statement
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Clone Note
The antibody binds specifically to IgE, an immunoglobulin isotype secreted by upon stimulation of B cells by interleukins Il-4 and IL-13. IgE provides immunity to parasites such as helminths and Plasmodium falciparum. The immunoglobulin also plays a central role in the development of Type I hypersensitivity reactions and allergic diseases such as asthma, sinusitis and food allergies. These allergic responses are triggered by the binding of IgE to the high affinity receptor FcepsilonRI, expressed on the surface of basophils and mast cells. Binding of IgE causes cross-linking of the receptor, inducing degranulation and release of inflammatory mediators, particularly of histamine. The monoclonal antibody TES-C21 binds to secreted IgE and to IgE expressed on the surface of IgE-producing B cells, thereby inhibiting IgE binding to FcepsilonRI and the release of histamine from mast cells. The monoclonal antibody can have applications, such as for identifying and quantifying IgE-expressing B cells and IgE levels in serum samples, and therapeutic applications in the treatment of allergies. Furthermore, the antibody can conjugated to cytotoxic drugs and deliver them specifically to IgE-producing B cells.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Testing Data
(Binding curve of anti-IgE antibody TES-C21 to human IgE Fc domain ELISA Plate coated with human IgE Fc domain at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
Testing Data
(Binding curve of anti-IgE antibody TES-C21 to human IgE Fc domain ELISA Plate coated with human IgE Fc domain at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed. For detection, a 1:4000 dilution of HRP labelled anti-human kappa light chain antibody was used.)
Related Product Information for IgE recombinant antibody
Recombinant monoclonal antibody to IgE. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma TES-C21.
Product Categories/Family for IgE recombinant antibody
References
Kolbinger F, Saldanha J, Hardman N, Bendig MM. Humanization of a mouse anti-human IgE antibody: a potential therapeutic for IgE-mediated allergies. Protein Eng. 1993 Nov;6(8):971-80. PMID:8309946
Original Published Journal: Protein Eng. 1993 Nov;6(8):971-80.
Original Published Journal: Protein Eng. 1993 Nov;6(8):971-80.
Original Published PMID: 8309946
Publication Note: Describes the therapeutic potential of the murine monoclonal antibody TES-C21 in the treatment of IgE-mediated allergies, and the production of a humanized version of the antibody to reduce the immunogenicity in humans.
Original Published Journal: Protein Eng. 1993 Nov;6(8):971-80.
Original Published Journal: Protein Eng. 1993 Nov;6(8):971-80.
Original Published PMID: 8309946
Publication Note: Describes the therapeutic potential of the murine monoclonal antibody TES-C21 in the treatment of IgE-mediated allergies, and the production of a humanized version of the antibody to reduce the immunogenicity in humans.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
immunoglobulin epsilon chain
Protein Family
Similar Products
Product Notes
The IgE (Catalog #AAA488430) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-IgE [TES-C21] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgE can be used in a range of immunoassay formats including, but not limited to, Blocking (BL), ELISA (EIA). Researchers should empirically determine the suitability of the IgE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgE, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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