Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27 recombinant antibody
Anti-CD27 [LG.3A10]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Testing Data
(Binding curve of anti-CD27 antibody LG.3A10 to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
Testing Data
(Binding curve of anti-CD27 antibody LG.3A10 to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein (Pr00160-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.1 ng/ml was performed using. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody (Bio-Rad) was used.)
Original Published Journal: International immunology, April 1995, Vol.7(4), pp.551-7
Original Published Journal: International immunology, April 1995, Vol.7(4), pp.551-7
Publication Note: Describes the original use of this antibody to elucidate the distribution and co-stimulatory activity of CD27.
NCBI and Uniprot Product Information
Uniprot Description
CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Protein type: Receptor, cytokine; Membrane protein, integral; Apoptosis
Cellular Component: cell surface; membrane; cell; integral to membrane; external side of plasma membrane
Molecular Function: protein binding; caspase inhibitor activity
Biological Process: B cell proliferation; positive regulation of T cell differentiation; apoptosis; immunoglobulin secretion; positive regulation of JNK cascade; negative regulation of caspase activity; negative regulation of apoptosis; positive regulation of interferon-gamma biosynthetic process
Research Articles on CD27
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Product Notes
The CD27 cd27 (Catalog #AAA488454) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD27 [LG.3A10] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, Activating, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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