C ReProtein (CRP) Active Protein | CRP active protein

Active C Reactive Protein (CRP)

Cat. Num. AAA2105119

• Gene Names: CRP; PTX1
• Reactivity: Homo sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: Purity: >98% as determined by SDS-PAGE.; Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
• Synonyms: C ReProtein (CRP); Active C Reactive Protein (CRP); C-RP; PTX1; Pentraxin-Related; Pentraxin 1; CRP active protein

• Host: Human (Plasma)
• Reactivity: Homo sapiens (Human)
• Purity/Purification: Purity: >98% as determined by SDS-PAGE.; Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.
• Form/Format: 20mM Tris, 280mM NaCl, 5mM CaCl2, 0.09% NaN3, pH8.0. containing 5% Trehalose.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for CRP active protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Source: Natural Extract
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method).
• Traits: Freeze-dried powder
• Predicted isoelectric point: 5.5
• Usage: Reconstitute in 20mM Tris, 280mM NaCl, 5mMCaCl2, 0.09%NaN3 (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8°C for one month. ; Aliquot and store at -80°C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRP active protein

The binding activity of CRP with RPL23A.C Reactive Protein (CRP) is an annular (ring-shaped), pentameric protein, a member of the pentraxin family of proteins. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. CRP is synthesized by the liver in response to factors released by macrophages and fat cells (adipocytes). Besides, Ribosomal Protein L23A (RPL23A) has been identified as an interactor of CRP, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CRP and recombinant human RPL23A. Briefly, CRP were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to RPL23A-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CRP and RPL23A was shown in Figure 1, and this effect was in a dose dependent manner.

Product Categories/Family for CRP active protein

Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 55770842
• NCBI GeneID: 1401
• NCBI Accession #: NP_000558.2
• NCBI GenBank Nucleotide #: NM_000567.3
• UniProt Accession #: P02741
• Molecular Weight: Accurate Molecular Mass: 25.0kDa; Observe Molecular Mass: 25kDa
• NCBI Official Full Name: C-reactive protein isoform 1
• NCBI Official Synonym Full Names: C-reactive protein
• NCBI Official Symbol: CRP
• NCBI Official Synonym Symbols: PTX1
• NCBI Protein Information: C-reactive protein
• UniProt Protein Name: C-reactive protein
• Protein Family: Cryparin
• UniProt Gene Name: CRP
• UniProt Synonym Gene Names: PTX1
• UniProt Entry Name: CRP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. [provided by RefSeq, Sep 2009]

Uniprot Description

CRP: Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells. Belongs to the pentaxin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q23.2

Cellular Component: extracellular space; growth cone; extracellular region; filopodium

Molecular Function: choline binding; protein binding; protein homodimerization activity; low-density lipoprotein receptor binding; complement component C1q binding; cholesterol binding; low-density lipoprotein binding; virion binding; calcium ion binding

Biological Process: protein polymerization; defense response to Gram-positive bacterium; response to ethanol; negative regulation of vasodilation; wound healing; positive regulation of superoxide release; response to lead ion; response to hypoxia; acute-phase response; opsonization; inflammatory response; complement activation, classical pathway; aging