Activity Data
(IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFgamma in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFgamma in the cell supernatant was detected using an ELISA kit. INFgamma levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.)
Interleukin 18 (IL18) Active Protein | IL18 active protein
Active Interleukin 18 (IL18)
Activity Data
(IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFgamma in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFgamma in the cell supernatant was detected using an ELISA kit. INFgamma levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.)
Activity Data
(IL18 (interleukin 18) is a cytokine that shares similarities in structure with IL1, and is produced by macrophages and other cells. IL18 has been reported to stimulate T cells proliferation and NK cell activity, besides, murine IL18 has been found to enchance the production of INFgamma in spleen cells in vitro. Thus, a stimulation assay was conducted to detect the activity of IL18 using murine spleen cells. Briefly, murine spleen cells were seeded into wells of 6-well plates at a density of 2×106 cells/mL in RPMI-1640 with the addition of various concentrations of IL18. After incubation for 48 hours, the concentration of INFgamma in the cell supernatant was detected using an ELISA kit. INFgamma levels in the cell supernatant of spleen cells increased significantly after stimulated with IL18, the data was shown in Figure 1.)
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SDS-Page
Testing Data
Testing Data
Testing Data
Testing Data
NCBI and Uniprot Product Information
Accurate Molecular Weight: 19kDa
Uniprot Description
IL18: Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells. Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 9 A5.3|9 27.75 cM
Cellular Component: apical plasma membrane; cytoplasm; extracellular region; extracellular space
Molecular Function: cytokine activity
Biological Process: activation of protein kinase B activity; angiogenesis; cell proliferation; cholesterol homeostasis; detection of mechanical stimulus involved in sensory perception of pain; immune response; induction of apoptosis via death domain receptors; inflammatory response; interferon-gamma biosynthetic process; interferon-gamma production; interleukin-13 biosynthetic process; interleukin-6 production; lipopolysaccharide-mediated signaling pathway; MAPK cascade; natural killer cell activation; natural killer cell mediated cytotoxicity; negative regulation of myoblast differentiation; negative regulation of neutrophil apoptosis; negative regulation of peptidyl-tyrosine phosphorylation; neutrophil activation; positive regulation of activated T cell proliferation; positive regulation of apoptosis; positive regulation of chemokine production; positive regulation of collagen biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of interleukin-8 production; positive regulation of natural killer cell proliferation; positive regulation of neuron apoptotic process; positive regulation of NF-kappaB import into nucleus; positive regulation of NK T cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of superoxide anion generation; positive regulation of T-helper 2 cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of STAT protein; positive regulation of vascular permeability; regulation of cell adhesion; response to hypoxia; sleep; T-helper 1 type immune response
Research Articles on IL18
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Product Notes
The IL18 il18 (Catalog #AAA2097227) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The Active Interleukin 18 (IL18) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 18 (IL18) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the IL18 il18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 18 (IL18), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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