Western Blot (WB)
(Western Blot analysis of NUCB1 expression in transfected 293T cell line by NUCB1 polyclonal antibody. Lane 1: NUCB1 transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human Nucleobindin 1 Polyclonal Antibody | anti-NUCB1 antibody
Nucleobindin 1 (NUCB1, CALNUC, NUC, FLJ40471)
Gene Names
NUCB1; NUC; CALNUC
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Nucleobindin 1; Polyclonal Antibody; Nucleobindin 1 (NUCB1; CALNUC; NUC; FLJ40471); Anti -Nucleobindin 1 (NUCB1; anti-NUCB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NUCB1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MPPSGPRGTLLLLPLLLLLLLRAVLAVPLERGAPNKEETPATESPDTGLYYHRYLQEVIDVLETDGHFREKLQAANAEDIKSGKLSRELDFVSHHVRTKLDELKRQEVSRLRMLLKAKMDAEQDPNVQVDHLNLLKQFEHLDPQNQHTFEARDLELLIQTATRDLAQYDAAHHEEFKRYEMLKEHERRRYLESLGEEQRKEAERKLEEQQRRHREHPKVNVPGSQAQLKEVWEELDGLDPNRFNPKTFFILHDINSDGVLDEQELEALFTKELEKVYDPKNEEDDMREMEEERLRMREHVMKNVDTNQDRLVTLEEFLASTQRKEFGDTGEGWETVEMHPAYTEEELRRFEEELAAREAELNAKAQRLSQETEALGRSQGRLEAQKRELQQAVLHMEQRKQQQQQQQGHKAPAAHPEGQLKFHPDTDDVPVPAPAGDQKEVDTSEKKLLERLPEVEVPQHL
Applicable Applications for anti-NUCB1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human NUCB1, aa1-461 (NP_006175.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of NUCB1 expression in transfected 293T cell line by NUCB1 polyclonal antibody. Lane 1: NUCB1 transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NUCB1 expression in transfected 293T cell line by NUCB1 polyclonal antibody. Lane 1: NUCB1 transfected lysate (53.9kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-NUCB1 antibody
This protein is a major calcium-binding protein of the Golgi. It may have a role in calcium homeostasis. It is located in the cis-Golgi, tightly associated with the luminal side of the membrane. A small fraction of the protein may be cytoplasmic.
Product Categories/Family for anti-NUCB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
53,879 Da
NCBI Official Full Name
Nucleobindin 1
NCBI Official Synonym Full Names
nucleobindin 1
NCBI Official Symbol
NUCB1
NCBI Official Synonym Symbols
NUC; CALNUC
NCBI Protein Information
nucleobindin-1
UniProt Protein Name
Nucleobindin-1
Protein Family
UniProt Gene Name
NUCB1
UniProt Synonym Gene Names
NUC
UniProt Entry Name
NUCB1_HUMAN
NCBI Description
This gene encodes a member of a small calcium-binding EF-hand protein family. The encoded protein is thought to have a key role in Golgi calcium homeostasis and Ca(2+)-regulated signal transduction events. [provided by RefSeq, Jun 2010]
Uniprot Description
NUCB1: Major calcium-binding protein of the Golgi. May have a role in calcium homeostasis. Belongs to the nucleobindin family.
Protein type: DNA-binding
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: microtubule cytoskeleton; Golgi apparatus; extracellular space; membrane; ER-Golgi intermediate compartment
Molecular Function: protein binding; DNA binding; calcium ion binding
Research Articles on NUCB1
Similar Products
Product Notes
The NUCB1 nucb1 (Catalog #AAA6012629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nucleobindin 1 (NUCB1, CALNUC, NUC, FLJ40471) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nucleobindin 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the NUCB1 nucb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MPPSGPRGTL LLLPLLLLLL LRAVLAVPLE RGAPNKEETP ATESPDTGLY YHRYLQEVID VLETDGHFRE KLQAANAEDI KSGKLSRELD FVSHHVRTKL DELKRQEVSR LRMLLKAKMD AEQDPNVQVD HLNLLKQFEH LDPQNQHTFE ARDLELLIQT ATRDLAQYDA AHHEEFKRYE MLKEHERRRY LESLGEEQRK EAERKLEEQQ RRHREHPKVN VPGSQAQLKE VWEELDGLDP NRFNPKTFFI LHDINSDGVL DEQELEALFT KELEKVYDPK NEEDDMREME EERLRMREHV MKNVDTNQDR LVTLEEFLAS TQRKEFGDTG EGWETVEMHP AYTEEELRRF EEELAAREAE LNAKAQRLSQ ETEALGRSQG RLEAQKRELQ QAVLHMEQRK QQQQQQQGHK APAAHPEGQL KFHPDTDDVP VPAPAGDQKE VDTSEKKLLE RLPEVEVPQH L. It is sometimes possible for the material contained within the vial of "Nucleobindin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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