Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
650 results for "Tag mAb" - showing 1-50
Test Data
(ELISA plate was coated by N protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein (Cat# AAA8574726)
Full Name
2019-nCov Nucleocapsid Recombinant Protein (full length)
Applications
EIA
Purity
Purity: >95% by SDS-page
Purification: Ni column
Purification: Ni column
Pricing
$475/0.1 mg | $2,095/5x0.1 mg
M13 + fd + F1 Filamentous Phages, Monoclonal Antibody (Cat# AAA531587)
Full Name
M13/fd/F1 FP mAb(HRP)
Reactivity
B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT
Applications
EIA
Purity
Protein A affinity chromatography
Sequence
Mannose Binding Lectin (MBL), Active Protein (Cat# AAA2086086)
Full Name
Active Mannose Binding Lectin (MBL)
Gene Names
MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
$155/0.01 mg | $275/0.05 mg | $415/0.1 mg | $505/0.2 mg | $970/0.5 mg | $1,425/1 mg | $3,490/5 mg | $15,690/5x5 mg
Hexahistidine Peptide 6HIS (HHHHHH) Epitope Tag, Monoclonal Antibody (Cat# AAA311213)
Full Name
MAb to 6HIS Peptide
Applications
EIA/ELISA, Immunoprecipitation, Western Blot
Purity
>=95% pure (SDS-PAGE). Protein G chromatography
Pricing
$555/1 mg | $2,355/5x1 mg
Western Blot (WB)
(Western blot detection of EGFP/EYFP expression in Rosetta E Coli cells induced by 0 (A)or 0.1mM (B)IPTG using EGFP/EYFP mouse mAb (1:1000 diluted).Predicted band size: 30KDa Observed band size: 30KDa.)
GFP/eGFP-tag, Monoclonal Antibody (Cat# AAA475146)
Full Name
Anti-GFP/eGFP-tag Mouse mAb
Reactivity
Transfected
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody (MBS125007) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.)
His-Tag, Antibody (Cat# AAA125007)
Full Name
His-Tag Monoclonal Antibody
Applications
WB, IP
Purity
Affinity Purification
Pricing
$175/0.05 mL | $215/0.1 mL | $285/0.2 mL | $1,130/5x0.2 mL
Sequence
Cold Inducible RNA Binding Protein (CIRBP), Active Protein (Cat# AAA2097238)
Full Name
Active Cold Inducible RNA Binding Protein (CIRBP)
Gene Names
CIRBP; CIRP
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>95%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Testing Data
(Representative western blot (1:1000) using HeLa cell lysate.)
GAPDH, Monoclonal Antibody (Cat# AAA330060)
Full Name
Anti-GAPDH mAb
Gene Names
Gapdh; Gapd
Reactivity
Recognize GAPDH from human, mouse, rat, rabbit, BL-21 Bacteria, Sf9 Insect cells, and Saccharomyces cerevisiae Yeast. GAPDH from other species may also be detectable.
Applications
EIA, DB, WB
Pricing
$300/0.1 mg | $1,185/1 mg | $5,095/5x1 mg
ELISA (EIA)
(ELISA plate was coated by N1 protein, 100 uL/cell, at various concentrations. The direct ELISA analysis was performed by loading 100 uL per well of a anti C-ter His tag HRP conjugated mAb at a concentration of 1:2000. The plate was incubated for 1 hours at 37 degree C, then washed 5 time. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Absorbances were read on a spectrophotometer at 450 nm.)
COVID 19 Nucleocapsid (NP) N1 fragment (N-ter domain) Coronavirus, Recombinant Protein (Cat# AAA8574729)
Full Name
2019-nCov Nucleocapsid Recombinant Protein N1 fragment (N-ter domain)
Applications
EIA
Purity
>95% by SDS-page
Pricing
$475/0.1 mg | $2,095/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with TAG-72 Monoclonal Antibody (SPM536).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438364)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescent analysis of GFP using either natural fluorescence (green) or an GFP antibody (red) in Hela(human cervical epithelial adenocarcinoma cell line) cells transfected with GFP recombinant protein. Formalin-fixed cells were permeabilized with 0. 1% Triton X-100 for 10 minutes at room temperature and blocked with 3% BSA for 30 minutes at room temperature. Cells were probed with an GFP monoclonal antibody (Product # MBS9200313) at a dilution of 1:25 for 1 hour at 37°C, and incubated with DyLight 555 goat anti-mouse IgG secondary antibody (Product # please inquire) at a dilution of 1:200 for 60 minutes at 37°C. The nuclear counter stain is DAPI (blue).)
GFP Tag, Monoclonal Antibody (Cat# AAA9200313)
Full Name
GFP Tag Antibody
Reactivity
Human
Applications
IF, EIA, WB
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with TAG-72 Monoclonal Antibody (SPM148).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438231)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Hamster, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(1ug DYKDDDDK fusion protein+ Primary antibody dilution at 1:10,000.)
DYKDDDDK-Tag, Monoclonal Antibody (Cat# AAA9400453)
Full Name
DYKDDDDK-Tag Mouse Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IF, IP
Purity
Affinity purification using immunogen.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate)
SNAP25, Monoclonal Antibody (Cat# AAA200064)
Full Name
SNAP25 antibody
Gene Names
SNAP25; SUP; RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
Reactivity
Human, Mouse
Applications
EIA, WB, Cell lysate
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CA72/733).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438423)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(1ug VSV-G fusion protein+ Primary antibody dilution at 1, 1:5,000 (lane 1) and 1:2,000 (lane 2))
VSV-G-Tag, Monoclonal Antibody (Cat# AAA8505639)
Full Name
VSV-G-Tag Mouse Monoclonal Antibody
Applications
WB, IP, IF
Pricing
$240/0.1 mg | $1,030/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of recombinant protein with E12-195 mAb diluted at 1:5, 000.)
Strep Tag II, Monoclonal Antibody (Cat# AAA8500737)
Full Name
Strep Tag II Mouse Monoclonal Antibody (Mix-mA)
Applications
WB: 1:2, 000-5, 000
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells (D) or CHO-K1 cells transfected with different DYKDDDDK-fusion proteins (A?B?C), using DYKDDDDK-Tag mouse mAb (1:1000 diluted).)
DYKDDDDK-tag, Monoclonal Antibody (Cat# AAA475238)
Full Name
Anti-DYKDDDDK-tag Mouse mAb
Reactivity
Transfected
Applications
WB, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (CA72/733).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438213)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (CC49).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438306)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, rat RAGE shares similarities with human RAGE in amino acids sequence with the identity of 80.0%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097246)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; S100P
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells (C, F) or CHO-K1 cells transfected with different HA-fusion proteins (A, B, D, E), using HA-Tag mouse mAb (1:1000 diluted).)
HA-tag, Monoclonal Antibody (Cat# AAA475212)
Full Name
Anti-HA-tag Mouse mAb
Reactivity
Transfected
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot detection of MEK-2 in THP-1, Jurkat, Raji, 3T3 and Hela cell lysates using 310001 (MEK-2 rabbit pAb, 1:1000 diluted), then incubated with 201069 (Rabbit IgG mAb, 1:50000 diluted).Anti-mouse IgG, HRP-linked Antibody was used for detecting.Predicted band size:44KDa.Observed band size:44KDa.)
IgG, Monoclonal Antibody (Cat# AAA475172)
Full Name
Anti-Rabbit IgG Mouse mAb
Reactivity
Rat
Applications
WB, EIA
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Western Blot (WB)
(Figure 1: Western blot analysis using KDR mouse mAb against extracellular domain of human KDR (aa20-764).)
KDR, Antibody (Cat# AAA850780)
Full Name
KDR Antibody
Gene Names
KDR; FLK1; CD309; VEGFR; VEGFR2
Reactivity
Human
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3 + CC49).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438380)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Activity Data
(CYP1A2 (Cytochrome P450 1A2) belongs to the group of proteins which contains heme as a cofactor. CYP1A2 oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Besides, ASAH1 (Acid ceramidase) has been identified as an interactor of CYP1A2 through affinity capture-MS. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human CYP1A2 and recombinant human ASAH1. Briefly, CYP1A2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ASAH1-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CYP1A2 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CYP1A2 and ASAH1 was shown in Figure 1, and this effect was in a dose dependent manner.)
Cytochrome P450 1A2 (CYP1A2), Active Protein (Cat# AAA2097310)
Full Name
Active Cytochrome P450 1A2 (CYP1A2)
Gene Names
CYP1A2; CP12; CYPIA2; P3-450; P450(PA)
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$265/0.01 mg | $595/0.05 mg | $920/0.1 mg | $1,135/0.2 mg | $2,240/0.5 mg | $3,315/1 mg | $8,120/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with TAG-72 Monoclonal Antibody (B72.3).)
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein), Monoclonal Antibody (Cat# AAA438112)
Full Name
TAG-72 / CA72.4 (Tumor-Associated Glycoprotein) Mouse Monoclonal Antibody
Reactivity
Human, Cow, Dog, Hamster, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
His-Tag, Monoclonal Antibody (Cat# AAA435058)
Full Name
His-Tag (2A8) mAb
Reactivity
All
Applications
WB, IF, IP, EIA
Pricing
$240/0.05 mL | $315/0.1 mL | $430/0.2 mL | $1,475/1 mL | $6,340/5x1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with SOX10 Monoclonal Antibody (SOX10/991).)
SOX10, Monoclonal Antibody (Cat# AAA439597)
Full Name
SOX10 (Melanoma Marker) Mouse Monoclonal Antibody
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Human, Mouse
Applications
FC, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(S100A6 is a calcium-binding protein, which functions as calcium sensor and modulator, contributing to cellular calcium signaling. It is reported that S100B and S100A6 differentially modulate cell survival by Interacting with distinct RAGE (Receptor for Advanced Glycation End Products). Thus a binding ELISA assay was conducted to detect the interaction of S100A6 and RAGE. Briefly, recombinant human S100A6 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100A6 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100A6 and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein A6 (S100A6), Active Protein (Cat# AAA2097347)
Full Name
Active S100 Calcium Binding Protein A6 (S100A6)
Gene Names
S100A6; 2A9; PRA; 5B10; CABP; CACY; S10A6
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with SOX10 Monoclonal Antibody (SOX10/1074).)
SOX10, Monoclonal Antibody (Cat# AAA439095)
Full Name
SOX10 (Melanoma Marker) Mouse Monoclonal Antibody
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Immunohistochemistry (IHC)
(Anti-LEFTY2 antibody IHC of human uterus, myometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
TGFB4 / LEFTY2, Monoclonal Antibody (Cat# AAA245271)
Full Name
Mouse Monoclonal [clone 7C5G1H6H10] (IgG2a,k) to Human TGFB4 / LEFTY2
Gene Names
LEFTY2; EBAF; LEFTA; TGFB4; LEFTYA
Reactivity
Human
Applications
IHC - Paraffin, WB, EIA
Purity
Protein A Purified
Pricing
$640/0.05 mg | $2,815/5x0.05 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with SOX10 Monoclonal Antibody (SPM607).)
SOX10, Monoclonal Antibody (Cat# AAA439788)
Full Name
SOX10 (Melanoma Marker) Mouse Monoclonal Antibody
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
HA-Tag, Monoclonal Antibody (Cat# AAA435083)
Full Name
HA-Tag (26D11) Mouse mAb
Reactivity
All
Applications
WB, IF, IP, EIA
Pricing
$240/0.05 mL | $315/0.1 mL | $430/0.2 mL | $1,475/1 mL | $6,340/5x1 mL
Western Blot (WB)
(Western blot analysis of over-expressed Myc-tagged protein in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000 (lane 2). Each lane was loaded with 10 ug of cell lysate.)
Myc-Tag, Monoclonal Antibody (Cat# AAA435061)
Full Name
Myc-Tag (19C2) Mouse mAb
Reactivity
All
Applications
WB, IF, IP, EIA
Pricing
$240/0.05 mL | $315/0.1 mL | $430/0.2 mL | $1,475/1 mL | $6,340/5x1 mL
Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
DsbA-tag, Monoclonal Antibody (Cat# AAA475026)
Full Name
Anti-DsbA-tag Mouse mAb
Reactivity
E Coli
Applications
WB-Re
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Activity Data
(Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Besides, mouse RAGE shares similarities with human RAGE in amino acids sequence with the identity of 78.2%. Thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2097249)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100b; Bpb; AI850290
Reactivity
Mouse
Applications
Cell culture, Activity Assays
Purity
> 90%
Pricing
$175/0.01 mg | $330/0.05 mg | $505/0.1 mg | $620/0.2 mg | $1,195/0.5 mg | $1,775/1 mg | $4,350/5 mg
Western Blot (WB)
(NANOG western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NANOG antibody detected the NANOG protein (arrow).)
NANOG, Monoclonal Antibody (Cat# AAA9200080)
Full Name
NANOG Antibody
Reactivity
Human
Applications
WB, EIA, IHC
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
GFP-Tag, Monoclonal Antibody (Cat# AAA435080)
Full Name
GFP-Tag (7G9) Mouse mAb
Reactivity
All
Applications
WB, IF, IP, EIA
Pricing
$240/0.05 mL | $315/0.1 mL | $430/0.2 mL | $1,475/1 mL | $6,340/5x1 mL
Western Blot (WB)
(Western blot analysis of over-expressed DDDDK-tagged protein in 293T cell using DDDDK antibody at different dilution. Each lane was loaded with 2 ug cell lysate.)
DDDDK-Tag, Monoclonal Antibody (Cat# AAA125006)
Full Name
DDDDK-Tag Monoclonal Antibody
Applications
WB, IHC, ICC, IP
Purity
Affinity Purification
Pricing
$185/0.05 mL | $240/0.1 mL | $350/0.2 mL | $1,420/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells, untransfected (C) or transfected with different DYKDDDDK-fusion proteins (A, B), using DYKDDDDK-tag-HRP (HRP-Conjugate) Mouse mAb (1:2000 diluted).)
DYKDDDDK-tag-HRP, Monoclonal Antibody (Cat# AAA477387)
Full Name
Anti-DYKDDDDK-tag-HRP (HRP-Conjugate) Mouse mAb
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Western blot analysis using AXL mouse mAb against extracellular domain of human AXL (aa19-444).)
AXL, Monoclonal Antibody (Cat# AAA475905)
Full Name
Anti-AXL Mouse mAb
Gene Names
AXL; ARK; UFO; JTK11; Tyro7
Reactivity
Human
Applications
WB, ICC
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis using AXL mouse mAb against extracellular domain of human AXL (aa19-444). Figure 1. Western blot a)
AXL, Antibody (Cat# AAA850888)
Full Name
AXL Antibody
Gene Names
AXL; ARK; UFO; JTK11; Tyro7
Reactivity
Human
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Activity Data
(Figure. The binding activity of S100B with RAGE.Protein S100B is a member of the S100 family. S100 proteins are EF-hand calcium-binding proteins and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Experimental results suggest that the receptor for advanced glycation end products (RAGE) plays important roles in mediating S100 protein-induced cellular signaling. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human S100B and recombinant human RAGE. Briefly, S100B were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RAGE-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-S100B mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of S100B and RAGE was shown in Figure 1, and this effect was in a dose dependent manner.)
S100 Calcium Binding Protein B (S100B), Active Protein (Cat# AAA2105105)
Full Name
Active S100 Calcium Binding Protein B (S100B)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Applications
Cell Culture, Activity Assays
Purity
> 90%
Pricing
$195/0.01 mg | $390/0.05 mg | $600/0.1 mg | $735/0.2 mg | $1,425/0.5 mg | $2,120/1 mg | $5,215/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis using GFP mouse mAb against recombinant GFP fusion protein (1) and various recombinant fusion protein with GFP tag (2, 3, 4).)
GFP, Antibody (Cat# AAA850015)
Full Name
GFP Antibody
Applications
WB, IF, FC/FACS, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
