Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells (C, F) or CHO-K1 cells transfected with different HA-fusion proteins (A, B, D, E), using HA-Tag mouse mAb (1:1000 diluted).)
anti-Transfected HA-tag Monoclonal Antibody | anti-HA antibody
Anti-HA-tag Mouse mAb
Reactivity
Transfected
Applications
Western Blot
Purity
Affinity Purified
Synonyms
HA-tag; Monoclonal Antibody; Anti-HA-tag Mouse mAb; anti-HA antibody
Host
Mouse
Source: Artificial tag
Source: Artificial tag
Reactivity
Transfected
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3C6-F8-G8
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide, and 50% glycerol.
Concentration
1.1mg/ml (varies by lot)
Sequence Length
10
Applicable Applications for anti-HA antibody
Western Blot (WB)
Application Notes
WB: 1:1000
IP: 1:100
IP: 1:100
Immunogen
HA synthetic peptide (YPYDVPDYA)conjugated to KLH.
Sensitivity
Transfected Only.
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells (C, F) or CHO-K1 cells transfected with different HA-fusion proteins (A, B, D, E), using HA-Tag mouse mAb (1:1000 diluted).)
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells (C, F) or CHO-K1 cells transfected with different HA-fusion proteins (A, B, D, E), using HA-Tag mouse mAb (1:1000 diluted).)
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates transfected with HA-tagged fusion protein using HA-Tag mouse mAb (1:100 diluted). HA-Tag mAb (201113) was used for the western blot analysis (1:2000 diluted))
Immunoprecipitation (IP)
(Immunoprecipitation analysis of Hela cell lysates transfected with HA-tagged fusion protein using HA-Tag mouse mAb (1:100 diluted). HA-Tag mAb (201113) was used for the western blot analysis (1:2000 diluted))
Related Product Information for anti-HA antibody
Entrez Summary: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques.Because of their small size, they are unlikely to affect the tagged protein?s biochemical properties.The YPYDVPDYA peptide has been used extensively as a general epitope tag in expression vectors.This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.
Product Categories/Family for anti-HA antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The HA (Catalog #AAA475212) is an Antibody produced from Mouse Source: Artificial tag and is intended for research purposes only. The product is available for immediate purchase. The Anti-HA-tag Mouse mAb reacts with Transfected and may cross-react with other species as described in the data sheet. AAA Biotech's HA-tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000 IP: 1:100. Researchers should empirically determine the suitability of the HA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HA-tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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