Western Blot (WB)
Mouse anti-All GFP-Tag Monoclonal Antibody
GFP-Tag (7G9) Mouse mAb
Reactivity
All
Applications
Western Blot, Immunofluorescence, Immunoprecipitation, ELISA
Synonyms
GFP-Tag; Monoclonal Antibody; GFP-Tag (7G9) Mouse mAb; anti-GFP-Tag antibody
Host
Mouse
Reactivity
All
Clonality
Monoclonal
Isotype
IgG1
Specificity
GFP-Tag (7G9) Mouse mAb detects GFP and GFP fusion proteins.
Sequence Length
304
Applicable Applications for anti-GFP-Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
Application Notes
WB: 1:5000
IF: 1:500
IP: 1:50
ELISA: 1:2000
IF: 1:500
IP: 1:50
ELISA: 1:2000
Source Note
This monoclonal antibody is produced by immunizing animals with full length recombinant GFP.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Western Blot (WB)
Western Blot (WB)
Immunofluorescence (IF)
Immunofluorescence (IF)
Immunoprecipitation (IP)
Immunoprecipitation (IP)
Related Product Information for anti-GFP-Tag antibody
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Product Categories/Family for anti-GFP-Tag antibody
References
1. Prasher, D.C., et al. 1992. Primary structure of the Aequorea victoria green fluorescent protein. Gene 111: 229-233.
2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
2. Chalfie, M., et al. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
GFP tag fusion protein, partial
Similar Products
Product Notes
The GFP-Tag (Catalog #AAA435080) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GFP-Tag (7G9) Mouse mAb reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's GFP-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA). WB: 1:5000 IF: 1:500 IP: 1:50 ELISA: 1:2000. Researchers should empirically determine the suitability of the GFP-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFP-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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