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Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody (MBS125007) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.)
Mouse His-Tag Antibody | anti-HIS-Tag antibody
His-Tag Monoclonal Antibody
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Synonyms
His-Tag; His-Tag Monoclonal Antibody; His; His tag; His-tag; anti-HIS-Tag antibody
Host
Mouse
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-HIS-Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
WB: 1:2000 - 1:5000
IF: 1:50 - 1:200
IP: 1:50 - 1:100
IF: 1:50 - 1:200
IP: 1:50 - 1:100
Category
Tag antibody
Immunogen
A synthetic peptide corresponding to His tag
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody (MBS125007) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.)
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody (MBS125007) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.)
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein,using His-Tag antibody (MBS125007) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with EPCAM protein,using His-Tag antibody (MBS125007) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (MBS125007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (MBS125007) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
Western Blot (WB)
(Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (MBS125007). A mock served as negative control and overexpressed 293T cell lysate served as positive control.)
Western Blot (WB)
(Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using His-tag antibody (MBS125007). A mock served as negative control and overexpressed 293T cell lysate served as positive control.)
Related Product Information for anti-HIS-Tag antibody
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
Product Categories/Family for anti-HIS-Tag antibody
Similar Products
Product Notes
The HIS-Tag (Catalog #AAA125007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:2000 - 1:5000 IF: 1:50 - 1:200 IP: 1:50 - 1:100. Researchers should empirically determine the suitability of the HIS-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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