Mouse anti-All His-Tag Monoclonal Antibody

His-Tag (2A8) mAb

Cat. Num. AAA435058

• Reactivity: All
• Applications: Western Blot, Immunofluorescence, Immunoprecipitation, ELISA
• Synonyms: His-Tag; Monoclonal Antibody; His-Tag (2A8) mAb; anti-His-Tag antibody

• Host: Mouse
• Reactivity: All
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: His-Tag (2A8) Mouse mAb detects over-expressed or recombinant proteins containing the 6x His epitope tag.

• Applicable Applications for anti-His-Tag antibody: Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: WB: 1:5000; IF: 1:2000; IP: 1:100; ELISA: 1:2000
• Source Note: This monoclonal antibody is produced by immunizing mice with a 6xHis synthetic peptide (KLH-coupled).
• Preparation and Storage: Store at -20 degree C. Stable for one year from the date of shipment.

Western Blot (WB)

Immunofluorescence (IF)

Immunoprecipitation (IP)

Related Product Information for anti-His-Tag antibody

Plasmid vectors for the expression of coding regions of eukaryotic genes in bacterial, insect and mammalian hosts are in common usage; such expression vectors are frequently used to encode hybrid fusion proteins consisting of a eukaryotic target protein and a specialized region designed to aid in the purification and visualization of the target protein. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni -chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag. Visualization of such fusion proteins can be achieved by utilizing antibodies generated against specific peptide sequences downstream from the multiple cloning site.

Product Categories/Family for anti-His-Tag antibody

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References

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