Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
anti-E Coli DsbA-tag Monoclonal Antibody | anti-DsbA antibody
Anti-DsbA-tag Mouse mAb
Reactivity
E Coli
Applications
Western Blot
Purity
Affinity Purified
Synonyms
DsbA-tag; Monoclonal Antibody; Anti-DsbA-tag Mouse mAb; DSBA like thioredoxin domain containing protein; anti-DsbA antibody
Host
Mouse
Source: E Coli
Source: E Coli
Reactivity
E Coli
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1A2-D4-G10
Purity/Purification
Affinity Purified
Form/Format
Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)with 0.2% sodium azide, 0.1mg/mlBSA and 50% glycerol.
Concentration
0.5mg/ml (varies by lot)
Sequence Length
208
Applicable Applications for anti-DsbA antibody
Western Blot (WB-Re)
Application Notes
WB: 1:1000
Immunogen
Purified recombinant E Coli. DsbA protein fragments over expressed in E Coli.
Sensitivity
This antibody detects endogenous levels of DsbA and does not cross-react with related proteins.
Sequence Similarities
Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.
Subcellular Location
Periplasm
Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
Western Blot (WB)
(Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.)
Western Blot (WB)
(Western blot detection of DSBA in AKT1, AKT2, AKT3 and DSBA recombinant antigen fragments using DSBA mouse mAb (1:1000 diluted).)
Western Blot (WB)
(Western blot detection of DSBA in AKT1, AKT2, AKT3 and DSBA recombinant antigen fragments using DSBA mouse mAb (1:1000 diluted).)
Related Product Information for anti-DsbA antibody
Entrez Summary: Disulphide oxidoreductase (DsbA) is the major oxidase responsible for generation of disulfide bonds in proteins of E Coli envelope. It is a member of the thioredoxin superfamily. DsbA introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24
NCBI Official Full Name
protein disulfide oxidoreductase
UniProt Protein Name
Thiol:disulfide interchange protein DsbA
Protein Family
UniProt Gene Name
dsbA
UniProt Synonym Gene Names
dsf; ppfA
Uniprot Description
Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Similar Products
Product Notes
The DsbA dsba (Catalog #AAA475026) is an Antibody produced from Mouse Source: E Coli and is intended for research purposes only. The product is available for immediate purchase. The Anti-DsbA-tag Mouse mAb reacts with E Coli and may cross-react with other species as described in the data sheet. AAA Biotech's DsbA-tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB-Re). WB: 1:1000. Researchers should empirically determine the suitability of the DsbA dsba for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DsbA-tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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