Testing Data
(1ug DYKDDDDK fusion protein+ Primary antibody dilution at 1:10,000.)
Mouse DYKDDDDK-Tag Monoclonal Antibody
DYKDDDDK-Tag Mouse Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification using immunogen.
Synonyms
DYKDDDDK-Tag; Monoclonal Antibody; DYKDDDDK-Tag Mouse Monoclonal Antibody; anti-DYKDDDDK-Tag antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Purity/Purification
Affinity purification using immunogen.
Form/Format
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-DYKDDDDK-Tag antibody
Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Western blotting: 1:5000~1:10000
Immunofluorescence: 1:2000
Immunoprecipitation: 1:5000
Immunofluorescence: 1:2000
Immunoprecipitation: 1:5000
Target Name
DYKDDDDK-tag
Specifity
The DYKDDDDK tag antibody can recognize C-terminal, internal and N-terminal DYKDDDDK-tag fusion proteins.
Preparation and Storage
Store at -20°C
Testing Data
(1ug DYKDDDDK fusion protein+ Primary antibody dilution at 1:10,000.)
Testing Data
(1ug DYKDDDDK fusion protein+ Primary antibody dilution at 1:10,000.)
Immunofluorescence (IF)
(IF analysis of 293 cells transfected with a DYKDDDDK-tag protein, using anti-DYKDDDDK-Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-DYKDDDDK).)
Immunofluorescence (IF)
(IF analysis of 293 cells transfected with a DYKDDDDK-tag protein, using anti-DYKDDDDK-Tag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-DYKDDDDK).)
Immunoprecipitation (IP)
(IP antibody use 15 ug DYKDDDDK Mouse IgG1 per ml Lysate WB 1:50001 B'B untransfected 293 cell lysate2 B'B transfected 293 cell lysate with DYKDDDDK-tag fusion protein 3 B'B'IP(transfected 293+ normal Mouse IgG+Protein G agarose) 4 B'B'IP (transfected 293+anti- DYKDDDDK mAb+ Protein G agarose)5 B'B'IP(transfected 293+Protein G) 6 B'B'Recombinant protein (E Coli).)
Immunoprecipitation (IP)
(IP antibody use 15 ug DYKDDDDK Mouse IgG1 per ml Lysate WB 1:50001 B'B untransfected 293 cell lysate2 B'B transfected 293 cell lysate with DYKDDDDK-tag fusion protein 3 B'B'IP(transfected 293+ normal Mouse IgG+Protein G agarose) 4 B'B'IP (transfected 293+anti- DYKDDDDK mAb+ Protein G agarose)5 B'B'IP(transfected 293+Protein G) 6 B'B'Recombinant protein (E Coli).)
Related Product Information for anti-DYKDDDDK-Tag antibody
The DYKDDDDK peptide (DYKDDDDK-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A DYKDDDDK-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a DYKDDDDK-tag to this protein allows one to follow the protein with an antibody against the DYKDDDDK sequence.
Product Categories/Family for anti-DYKDDDDK-Tag antibody
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Product Notes
The DYKDDDDK-Tag (Catalog #AAA9400453) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DYKDDDDK-Tag Mouse Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DYKDDDDK-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). Western blotting: 1:5000~1:10000 Immunofluorescence: 1:2000 Immunoprecipitation: 1:5000. Researchers should empirically determine the suitability of the DYKDDDDK-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYKDDDDK-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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