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Western Blot (WB) (Anti-ZADH2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa)

Rabbit anti-Human ZADH2 Polyclonal Antibody | anti-Zadh2 antibody

Anti-ZADH2 Antibody, Rabbit Polyclonal

Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
ZADH2; Polyclonal Antibody; Anti-ZADH2 Antibody; Rabbit Polyclonal; ZADH2 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-Zadh2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ZADH2
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-Zadh2 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human ZADH2 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ZADH2 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-ZADH2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa)

Western Blot (WB) (Anti-ZADH2 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:40 kDa Observed band size:44 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human ZADH2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC) (Immunochemical staining of human ZADH2 in human liver with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-Zadh2 antibody
ZADH2 (Zinc Binding Alcohol Dehydrogenase Domain Containing 2) is located on human chromosome 18q22.3. The ZADH2 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, A.thaliana, rice, and frog. 238 organisms have orthologs with human gene ZADH2. ZADH2 overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation. ZADH2 is associated with some diseases, including Brachydactyly, Type D and Vertical Talus, Congenital. ZADH2 is related to oxidoreductase activity and transferase activity, transferring acyl groups other than amino-acyl groups. An important paralog of this gene is PTGR1.
References
Madrid A, et al. (2018) DNA hypomethylation in blood links b3galt4 and zadh2 to alzheimer's disease. J Alzheimers Dis 66 (3): 927-934.

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Product Notes

The Zadh2 (Catalog #AAA8109191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZADH2 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZADH2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the Zadh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZADH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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