Western Blot (WB)
(Anti-MRPL19 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa)
Rabbit anti-Human MRPL19 Polyclonal Antibody | anti-MRPL19 antibody
Anti-MRPL19 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
MRPL19; Polyclonal Antibody; Anti-MRPL19 Antibody; Rabbit Polyclonal; MRPL19 Antibody; Rabbit PAb; Antigen Affinity Purified; mitochondrial ribosomal protein L19; Anti-L19mt Antibody; Anti-MRP-L15 Antibody; Anti-MRP-L19 Antibody; Anti-MRPL15 Antibody; Anti-RLX1 Antibody; Anti-RPML15 Antibody; anti-MRPL19 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MRPL19
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-MRPL19 antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:200-1:1000
IP: 0.5-2uL/mg of lysate
IHC-P: 1:200-1:1000
IP: 0.5-2uL/mg of lysate
Immunogen
E Coli-derived Human MRPL19 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human MRPL19 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-MRPL19 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa)
Western Blot (WB)
(Anti-MRPL19 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lane D: Raji Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:34 kDa)
Immunohistochemistry (IHC)
(Immunochemical staining of human MRPL19 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human MRPL19 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)
Immunoprecipitation (IP)
(MRPL19 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-MRPL19 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPL19 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size :30 kDa)
Immunoprecipitation (IP)
(MRPL19 was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate 4 uL anti-MRPL19 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-MRPL19 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size :30 kDa)
Related Product Information for anti-MRPL19 antibody
MRPL19 (Mitochondrial Ribosomal Protein L19) is a Protein Coding gene. The MRPL19 gene, located on 2p12, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, and frog. MRPL19 belongs to the bacterial ribosomal protein bL19 family. This gene encodes a 39S subunit protein and is widely expressed in the thyroid, kidney, and other tissues. Diseases associated with MRPL19 include Reading Disorder and Dyslexia. Among its related pathways are Organelle biogenesis and maintenance and Viral mRNA Translation. Data support that MRPL19 and C2ORF3 are candidate susceptibility genes for DYX3. 243 organisms have orthologs with the human gene MRPL19.
References
Paracchini S, et al. (2011) Analysis of dyslexia candidate genes in the raine cohort representing the general australian population. Genes Brain Behav 10 (2): 158-165.Mascheretti S, et al. (2018) Beyond genes: A systematic review of environmental risk factors in specific reading disorder. Res Dev Disabil 82 147-152.Anthoni H, et al. (2007) A locus on 2p12 containing the co-regulated mrpl19 and c2orf3 genes is associated to dyslexia. Hum Mol Genet 16 (6): 667-677.
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Product Notes
The MRPL19 (Catalog #AAA8108634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MRPL19 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MRPL19 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP). WB: 1:500-1:2000 IHC-P: 1:200-1:1000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the MRPL19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPL19, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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