SDPR Recombinant Protein | CAVIN2 recombinant protein

SDPR Protein, Mouse, Recombinant (aa 2-180, His Tag)

Cat. Num. AAA8122864

• Purity: >95% as determined by SDS-PAGE
• Synonyms: SDPR; SDPR Protein; Mouse; Recombinant (aa 2-180; His Tag); Mouse SDPR Protein (aa 2-180; serum deprivation response; CAVIN2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4, 30% glycerol. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly2-Ala180

• Species: Mouse
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mouse SDPR (NP_620080.1) N-terminal segment (Gly 2-Ala 180) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for CAVIN2 recombinant protein

Background: Serum deprivation-response protein, also known as Phosphatidylserine-binding protein, Cavin-2 and SDPR, is a member of the PTRF/SDPR family. SDPR is highly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle. SDPR is a new regulator of caveolae biogenesis. SDPR is up-regulated in asynchronously growing fibroblasts following serum deprivation but not following contact inhibition and Down-regulated during synchronous cell cycle re-entry. Caveolae are plasma membrane invaginations with a characteristic flask-shaped morphology. They function in diverse cellular processes, including endocytosis. Loss of SDPR causes loss of caveolae. SDPR binds directly to PTRF and recruits PTRF to caveolar membranes. Overexpression of SDPR, unlike PTRF, induces deformation of caveolae and extensive tubulation of the plasma membrane. SDPR overexpression results in increased caveolae size and leads to the formation of caveolae-derived tubules containing Shiga toxin. SDPR is a membrane curvature inducing component of caveolae, and that STB-induced membrane tubulation is facilitated by caveolae. Pleckstrin and SDPR are phosphorylated by protein kinase C (PKC), the interaction between pleckstrin and SDPR was shown to be independent of PKC inhibition or activation. SDPR may facilitate the translocation of nonphosphorylated pleckstrin to the plasma membrane in conjunction with phosphoinositides that bind to the C-terminal PH domain.

References

Li,X. et al., 2008, Cancer Sci. 99 (7):1326-33. Hansen,C.G. et al., 2009, Nat Cell Biol. 11 (7):807-14. Baig,A. et al., 2009, Platelets. 20 (7):446-57. Nabi,IR. et al., 2009, Nat Cell Biol.11 (7):789-91.

NCBI and Uniprot Product Information

• NCBI GI #: 20270267
• NCBI GeneID: 20324
• NCBI Accession #: NP_620080.1
• NCBI GenBank Nucleotide #: NM_138741.1
• UniProt Accession #: Q63918; Q3V1P6; Q78EC3; Q8CBT4
• Molecular Weight: 46,764 Da
• NCBI Official Full Name: serum deprivation-response protein
• NCBI Official Synonym Full Names: serum deprivation response
• NCBI Official Symbol: Sdpr
• NCBI Protein Information: serum deprivation-response protein; cavin 2; cavin-2; phosphatidylserine-binding protein
• UniProt Protein Name: Serum deprivation-response protein
• UniProt Gene Name: Sdpr
• UniProt Synonym Gene Names: Sdr
• UniProt Entry Name: SDPR_MOUSE