Western Blot (WB)
(Anti-PPTC7 rabbit polyclonal antibody at 1:500 dilution Lane A: RPMI-8226 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:35 kDa)
Rabbit anti-Human PPTC7 Polyclonal Antibody | anti-PPTC7 antibody
Anti-PPTC7 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
PPTC7; Polyclonal Antibody; Anti-PPTC7 Antibody; Rabbit Polyclonal; PPTC7 Antibody; Rabbit PAb; Antigen Affinity Purified; PTC7 protein phosphatase homolog; Anti-TA-PP2C Antibody; Anti-TAPP2C Antibody; anti-PPTC7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human PPTC7
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-PPTC7 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
E Coli-derived Human PPTC7 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human PPTC7 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-PPTC7 rabbit polyclonal antibody at 1:500 dilution Lane A: RPMI-8226 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:35 kDa)
Western Blot (WB)
(Anti-PPTC7 rabbit polyclonal antibody at 1:500 dilution Lane A: RPMI-8226 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:33 kDa Observed band size:35 kDa)
Related Product Information for anti-PPTC7 antibody
PPTC7 (Protein Phosphatase Targeting COQ7) is a Protein Coding gene. The PPTC7 gene, located on 12q24.11, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, S.pombe, M.oryzae, N.crassa, A.thaliana, and frog. PPTC7 is a regulatory protein of CoQ10 biosynthesis required for human cell survival. PPTC7 is a bona fide protein phosphatase that dephosphorylates the human COQ7. PPTC7 is widely expressed in bone marrow, heart, and other tissues. Among its related pathways are Development Dopamine D2 receptor transactivation of EGFR and the Regulation of CFTR activity (norm and CF). 240 organisms have orthologs with the human gene PPTC7.
References
Mao M, et al. (2004) T lymphocyte activation gene identification by coregulated expression on DNA microarrays. Genomics 83 (6): 989-999.Gonzalez-Mariscal I, et al. (2018) The mitochondrial phosphatase pptc7 orchestrates mitochondrial metabolism regulating coenzyme q(10) biosynthesis. Biochim Biophys Acta Bioenerg 1859 (11): 1235-1248.Butland SL, et al. (2014) The palmitoyl acyltransferase hip14 shares a high proportion of interactors with huntingtin: Implications for a role in the pathogenesis of huntington's disease. Hum Mol Genet 23 (15): 4142-4160.
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Product Notes
The PPTC7 (Catalog #AAA8108926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PPTC7 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPTC7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the PPTC7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPTC7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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