Western Blot (WB)
(Anti-RNGTT rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa (We are unsure as to the identity of these extra bands.))
Rabbit anti-Human RNGTT Polyclonal Antibody | anti-RNGTT antibody
Anti-RNGTT Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
RNGTT; Polyclonal Antibody; Anti-RNGTT Antibody; Rabbit Polyclonal; RNGTT Antibody; Rabbit PAb; Antigen Affinity Purified; RNA guanylyltransferase and 5'-phosphatase; Anti-CAP1A Antibody; Anti-hCAP Antibody; Anti-HCE Antibody; Anti-HCE1 Antibody; anti-RNGTT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human RNGTT
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-RNGTT antibody
Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
IHC-P: 1:50-1:200
IHC-P: 1:50-1:200
Immunogen
E Coli-derived Human RNGTT fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human RNGTT fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-RNGTT rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa (We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-RNGTT rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa (We are unsure as to the identity of these extra bands.))
Immunohistochemistry (IHC)
(Immunochemical staining of human RNGTT in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RNGTT in human placenta with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RNGTT in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human RNGTT in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-RNGTT antibody
RNGTT (RNA Guanylyltransferase And 5'-Phosphatase) is a Protein Coding gene. The RNGTT gene, located on 6q15, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, A.thaliana, rice, and frog. The mammalian homolog of mRNA-cap, RNGTT, can replace mRNA-cap to play the same function in the Drosophila Hh pathway, and knockdown of Rngtt in cultured mammalian cells will impair the Shh pathway activity. RNGTT is widely expressed in the lymph node, testis, and other tissues. Diseases associated with RNGTT include Cecum Carcinoma and Cecum Adenocarcinoma. Among its related pathways are HIV Transcription Elongation and Gene Expression. 230 organisms have orthologs with the human gene RNGTT.
References
Trotman JB, et al. (2018) Rna-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome. J Biol Chem 293 (43): 16596-16607.Lombardi O, et al. (2016) C-myc deregulation induces mrna capping enzyme dependency. Oncotarget 7 (50): 82273-82288.Chen P, et al. (2017) Capping enzyme mrna-cap/rngtt regulates hedgehog pathway activity by antagonizing protein kinase a. Sci Rep 7 (1): 2891.
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Product Notes
The RNGTT (Catalog #AAA8109348) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RNGTT Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RNGTT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000 IHC-P: 1:50-1:200. Researchers should empirically determine the suitability of the RNGTT for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RNGTT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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