Western Blot (WB)
(Anti-TMEM109 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:22 kDa)
Rabbit anti-Human TMEM109 Polyclonal Antibody | anti-TMEM109 antibody
Anti-TMEM109 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
TMEM109; Polyclonal Antibody; Anti-TMEM109 Antibody; Rabbit Polyclonal; TMEM109 Antibody; Rabbit PAb; Antigen Affinity Purified; transmembrane protein 109; anti-TMEM109 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human TMEM109
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-TMEM109 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
IP: 0.5-2uL/mg of lysate
IP: 0.5-2uL/mg of lysate
Immunogen
E Coli-derived Human TMEM109 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human TMEM109 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-TMEM109 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:22 kDa)
Western Blot (WB)
(Anti-TMEM109 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:20 kDa Observed band size:22 kDa)
Immunoprecipitation (IP)
(TMEM109 was immunoprecipitated using: Lane A:0.5 mg U-251MG Whole Cell Lysate 4 uL anti-TMEM109 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TMEM109 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size :20 kDa)
Immunoprecipitation (IP)
(TMEM109 was immunoprecipitated using: Lane A:0.5 mg U-251MG Whole Cell Lysate 4 uL anti-TMEM109 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-TMEM109 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 20 kDa Observed band size :20 kDa)
Related Product Information for anti-TMEM109 antibody
Transmembrane protein 109 is a protein that in humans is encoded by the TMEM109 gene. TMEM109 maps on chromosome 11, at 11q12.2, and is conserved in chimpanzee, dog, cow, mouse, and rat. The gene is also known as MGC5508, LOC79073. It has been described as transmembrane protein 109, mg23, mitsugumin-23. TMEM109 may mediate cellular response to DNA damage by protecting against ultraviolet C-induced cell death and can form voltage-gated calcium and potassium channels in vitro. TMEM109 is widely expressed in fat, lung, and other tissues. Diseases associated with TMEM109 include Spinal Cord Astrocytoma. 162 organisms have orthologs with the human gene TMEM109.
References
Yamazaki T, et al. (2010) Facilitation of DNA damage-induced apoptosis by endoplasmic reticulum protein mitsugumin23. Biochem Biophys Res Commun 392 (2): 196-200.Vecchione A, et al. (2007) Protein partners in the life history of activated fibroblast growth factor receptors. Proteomics 7 (24): 4565-4578.Knopf JD, et al. (2015) The stromal cell-surface protease fibroblast activation protein-alpha localizes to lipid rafts and is recruited to invadopodia. Biochim Biophys Acta 1853 (10 Pt A): 2515-2525.
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Product Notes
The TMEM109 (Catalog #AAA8108749) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TMEM109 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM109 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 0.5-2uL/mg of lysate. Researchers should empirically determine the suitability of the TMEM109 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM109, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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