Western Blot (WB)
(Anti-WDR54 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa)
Rabbit anti-Human WDR54 Polyclonal Antibody | anti-WDR54 antibody
Anti-WDR54 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
WDR54; Polyclonal Antibody; Anti-WDR54 Antibody; Rabbit Polyclonal; WDR54 Antibody; Rabbit PAb; Antigen Affinity Purified; WD repeat domain 54; anti-WDR54 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human WDR54
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-WDR54 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
E Coli-derived Human WDR54 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human WDR54 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Anti-WDR54 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa)
Western Blot (WB)
(Anti-WDR54 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:36 kDa Observed band size:36 kDa)
Related Product Information for anti-WDR54 antibody
WDR54 (WD Repeat Domain 54) is a Protein Coding gene. The WDR54 gene, located on 2p13.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, and zebrafish. WDR54 is a member of the WD40 repeat (WDR) domain-containing protein family that was identified as a novel oncogene in colorectal cancer. WDR54 serves as an oncogene in colorectal cancer (CRC) and maybe a potential prognostic marker and therapeutic target. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing, and cytoskeleton assembly. WDR54 is widely expressed in the testis, lung, and other tissues. An important paralog of this gene is WDR64.
References
Yuan Y, et al. (2019) Clinical significance and biological function of wd repeat domain 54 as an oncogene in colorectal cancer. Int J Cancer 144 (7): 1584-1595.
Maeda A, et al. (2019) Transglutaminase-mediated cross-linking of wdr54 regulates egf receptor-signaling. Biochim Biophys Acta Mol Cell Res 1866 (2): 285-295.
Maeda A, et al. (2019) Transglutaminase-mediated cross-linking of wdr54 regulates egf receptor-signaling. Biochim Biophys Acta Mol Cell Res 1866 (2): 285-295.
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Product Notes
The WDR54 (Catalog #AAA8109051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-WDR54 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's WDR54 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the WDR54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WDR54, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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