Rabbit Tubulin beta Polyclonal Antibody

Anti-Tubulin beta

Cat. Num. AAA516283

• Reactivity: Western blot Tubulin B from human, mouse cells; Tubulin B from other species may also be detectable.
• Applications: Western Blot
• Purity: Affinity Chromatography
• Synonyms: Tubulin beta; Polyclonal Antibody; Anti-Tubulin beta; anti-Tubulin beta antibody

• Host: Rabbit
• Reactivity: Western blot Tubulin B from human, mouse cells; Tubulin B from other species may also be detectable.
• Clonality: Polyclonal
• Specificity: Recognizes the Tubulin beta protein
• Purity/Purification: Affinity Chromatography
• Form/Format: TBS, 50% glycerol

• Applicable Applications for anti-Tubulin beta antibody: Western Blot (WB)
• Application Notes: WB: 18127:19126 dilution
• Immunogen: Synthetic peptide derived from beta-tubulin of many animal species
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Representative western blot with Anti- Tubulin beta (1:1000) using 30 ug of HeLa, HEK-293T, Jurkat, THP1, 3T6 and K562 cell lysate.)

Related Product Information for anti-Tubulin beta antibody

TUBB1 or tubulin-beta 1 protein is a major constituent of microtubules. TUBB1 interaction with microtubule-associated proteins (MAPs) such as tau is fundamental for microtubule structure and function (1). Previous work suggested that the "microtubule binding domain" of tau (composed of three or four imperfect 18-amino acid repeats, separated by 13-or 14-amino acid inter-repeat regions) can bind to the C-terminal ends of both alpha and beta tubulin monomers. Studies revealed that TUBB1 is the target of various antitubulin agents used in the treatment of cancer. Subsequent studies have also concluded that TUBB1 mutations in clinical samples are rare, and unlikely to contribute to drug resistance (2).

Product Categories/Family for anti-Tubulin beta antibody

Primary Antibodies; Cardiovascular Disease; Neurobiology

References

1. Chau, M.F. et al: The microtubule-associated protein tau cross-links to two distinct sites on each alpha and beta tubulin monomer via separate domains.". Biochemistry, 1999; 37 (51): 17692-703.
2. Berrieman, H.K. et al: Do beta-tubulin mutations have a role in resistance to chemotherapy?"Lancet Oncol, 2004; 5 (3): 158-64.