Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation with 2 ug of Anti-BCL2 using 500 ug of HeLa, HEK-293T, and Jurkat cell lysate.)
Rabbit BCL2 Polyclonal Antibody | anti-BCL2 antibody
Anti-BCL2
Reactivity
Immunoprecipitation of BCL2 from human cellsWestern blot of BCL2 from human cells.
BCL2 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
BCL2; Polyclonal Antibody; Anti-BCL2; anti-BCL2 antibody
Host
Rabbit
Reactivity
Immunoprecipitation of BCL2 from human cells
Western blot of BCL2 from human cells.
BCL2 from other species may also be detectable.
Western blot of BCL2 from human cells.
BCL2 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the BCL2 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-BCL2 antibody
Western Blot (WB)
Application Notes
WB: 21080:22079
IP: 2ug
IP: 2ug
Immunogen
Synthetic peptide corresponding to amino acids 54-58 of human BCL2
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation with 2 ug of Anti-BCL2 using 500 ug of HeLa, HEK-293T, and Jurkat cell lysate.)
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation with 2 ug of Anti-BCL2 using 500 ug of HeLa, HEK-293T, and Jurkat cell lysate.)
Western Blot (WB)
(Sample Western Blot: Representative western blot with Anti-BCL2 (1:1000) using 20 ng of GST tagged human recombinant BCL2 (left image) or 3 ug, 10 ug, and 30 ug of Jurkat cell lysate..)
Western Blot (WB)
(Sample Western Blot: Representative western blot with Anti-BCL2 (1:1000) using 20 ng of GST tagged human recombinant BCL2 (left image) or 3 ug, 10 ug, and 30 ug of Jurkat cell lysate..)
Related Product Information for anti-BCL2 antibody
BCL2 gene encodes an integral inner mitochondrial membrane protein that acts as an antiapoptotic protein (1). The protein BAD can antagonize both the cell cycle and antiapoptotic functions of BCL2 through binding to the BH3 domain. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma (2). BCL2 is phosphorylated on specific serine/threonine residues within the unstructured loop in response to diverse stimuli and such phosphorylation has been associated with the loss of the biological function of BCL2.
Product Categories/Family for anti-BCL2 antibody
References
1. Hockenbery, D. et al: Bcl-2 is an inner mitochondrial membrane protein that blocks programmed cell death. Nature 348: 334-336, 1990.
2. Tsujimoto, Y. et al: Involvement of the bcl-2 gene in human follicular lymphoma. Science 228: 1440-1443, 1985.
2. Tsujimoto, Y. et al: Involvement of the bcl-2 gene in human follicular lymphoma. Science 228: 1440-1443, 1985.
Similar Products
Product Notes
The BCL2 (Catalog #AAA515874) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCL2 reacts with Immunoprecipitation of BCL2 from human cells Western blot of BCL2 from human cells. BCL2 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's BCL2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 21080:22079 IP: 2ug. Researchers should empirically determine the suitability of the BCL2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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