Immunoprecipitation (IP)
(Sample Data: Representative immunoprecipitation with 2 ug of Anti-STAT3 using 500 ug of HeLa, HEK-293T, Jurkat, 3T6, and THP1 cell lysate.)
Rabbit STAT3 Polyclonal Antibody | anti-STAT3 antibody
Anti-STAT3
Reactivity
Western blot of human STAT3STAT3 from other species may also be detectable
Applications
Western Blot, Immunoprecipitation
Purity
Affinity chromatography
Synonyms
STAT3; Polyclonal Antibody; Anti-STAT3; anti-STAT3 antibody
Host
Rabbit
Reactivity
Western blot of human STAT3
STAT3 from other species may also be detectable
STAT3 from other species may also be detectable
Clonality
Polyclonal
Specificity
Recognizes the STAT3 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-STAT3 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 21795:22794
IP: 2ug
IP: 2ug
Immunogen
Synthetic peptide corresponding to amino acids 725-729 of human STAT3
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunoprecipitation (IP)
(Sample Data: Representative immunoprecipitation with 2 ug of Anti-STAT3 using 500 ug of HeLa, HEK-293T, Jurkat, 3T6, and THP1 cell lysate.)
Immunoprecipitation (IP)
(Sample Data: Representative immunoprecipitation with 2 ug of Anti-STAT3 using 500 ug of HeLa, HEK-293T, Jurkat, 3T6, and THP1 cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT3 (1:1000) using 20 ng of human recombinant STAT1beta, STAT2, STAT3, STAT4, STAT5, and STAT6.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT3 (1:1000) using 20 ng of human recombinant STAT1beta, STAT2, STAT3, STAT4, STAT5, and STAT6.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT3 (1:1000) using 30 ug of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT3 (1:1000) using 30 ug of HeLa, HEK-293T, Jurkat, THP1, and 3T6 cell lysate.)
Related Product Information for anti-STAT3 antibody
STAT3 is a member of the signal transducers and activators of transcription (STAT) family of proteins that carry out a dual function: signal transduction and activation of transcription. STAT3 is widely expressed and becomes activated through phosphorylation on tyrosine as a DNA binding protein in response to a variety of stimuli such as EGF, IL-6, PDGF, IL-2 and G-CSF (1). This phosphoprotein forms homodimers as well as heterodimers with other members of the STAT family and translocate to the nucleus in order to modulate the transcription of various genes (2).
Product Categories/Family for anti-STAT3 antibody
References
1. Zhong, Z. et al: Stat3: a STAT family member activated by tyrosine phosphorylation in response to epidermal growth factor and interleukin-6. Science. 1994 Apr 1;264(5155):95-8.
2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760.
2. Tian, S. S. et al: Rapid activation of the STAT3 transcription factor by granulocyte colony-stimulating factor. Blood. 1994 Sep 15;84(6):1760.
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Product Notes
The STAT3 (Catalog #AAA516241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-STAT3 reacts with Western blot of human STAT3 STAT3 from other species may also be detectable and may cross-react with other species as described in the data sheet. AAA Biotech's STAT3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 21795:22794 IP: 2ug. Researchers should empirically determine the suitability of the STAT3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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