Rabbit HDAC4 Polyclonal Antibody | anti-HDAC4 antibody

Anti-HDAC4

Cat. Num. AAA516001

• Gene Names: HDAC4; HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• Reactivity: Western blot of HDAC4 from human, mouse cells; HDAC4 from other species may also be detectable.
• Applications: Western Blot
• Purity: Affinity Chromatography
• Synonyms: HDAC4; Polyclonal Antibody; Anti-HDAC4; anti-HDAC4 antibody

• Host: Rabbit
• Reactivity: Western blot of HDAC4 from human, mouse cells; HDAC4 from other species may also be detectable.
• Clonality: Polyclonal
• Specificity: Recognizes the HDAC4 protein
• Purity/Purification: Affinity Chromatography
• Form/Format: TBS, 50% glycerol

• Applicable Applications for anti-HDAC4 antibody: Western Blot (WB)
• Application Notes: WB: 20098:21097
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Representative western blot (1:1000) with Anti-HDAC4 using 3 ug, 10 ug, 30ug of Hela cell lysate.)

Related Product Information for anti-HDAC4 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing trans factors increased accessibility to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate non-histone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I (HDACs 1, 2, 3 and 8) proteins are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9 and 10) are related to yeast Hda1-like proteins and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).

Product Categories/Family for anti-HDAC4 antibody

Primary Antibodies; Cardiovascular Disease; WNT Signaling

References

1. Marmorstein, R. et al. (2001) Cell. Mol. Life Sci. 58, 693-703.
2. Gregory, P.D. et al. (2001) Exp. Cell Res. 265, 195-202.
3. Liu, Y. et al. (2000) Mol. Cell. Biol. 20, 5540-5543.
4. Cress, S.D. and Seto, E. (2000) J. Cell. Physiol. 184, 1-16.
5. Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262, 75-83.
6. Thiagalingam, S. et al. (2003) Ann. N. Y. Acad. Sci. 983, 84-100.
7. Viguishin, D.M. and Coombes, R.C. (2004) Curr. Cancer Drug Targets 4, 205-218.

NCBI and Uniprot Product Information

• NCBI GI #: 153085395
• NCBI GeneID: 9759
• NCBI Accession #: NP_006028.2
• NCBI GenBank Nucleotide #: NM_006037.3
• UniProt Accession #: P56524; Q9UND6
• Molecular Weight: 119,040 Da
• NCBI Official Full Name: histone deacetylase 4
• NCBI Official Synonym Full Names: histone deacetylase 4
• NCBI Official Symbol: HDAC4
• NCBI Official Synonym Symbols: HD4; AHO3; BDMR; HDACA; HA6116; HDAC-4; HDAC-A
• NCBI Protein Information: histone deacetylase 4; histone deacetylase A
• UniProt Protein Name: Histone deacetylase 4
• UniProt Gene Name: HDAC4
• UniProt Synonym Gene Names: KIAA0288; HD4
• UniProt Entry Name: HDAC4_HUMAN

NCBI Description

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC4: a transcriptional regulator of the histone deacetylase family, subfamily 2. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events. Does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Protein type: Nuclear receptor co-regulator; Hydrolase; Deacetylase; EC 3.5.1.98

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: nucleoplasm; transcriptional repressor complex; cytoplasm; histone deacetylase complex; actomyosin; neuromuscular junction; Z disc; cytosol; nucleus; A band

Molecular Function: potassium ion binding; NAD-dependent histone deacetylase activity (H3-K9 specific); protein binding; transcription activator binding; NAD-dependent histone deacetylase activity (H3-K14 specific); zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; protein deacetylase activity; NAD-dependent histone deacetylase activity (H4-K16 specific); histone deacetylase activity; protein kinase binding; transcription factor binding; transcription corepressor activity

Biological Process: cardiac muscle hypertrophy; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; response to denervation involved in regulation of muscle adaptation; regulation of gene expression, epigenetic; negative regulation of cell proliferation; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; negative regulation of glycolysis; inflammatory response; skeletal development; response to drug; nervous system development; regulation of skeletal muscle fiber development; B cell activation; transcription, DNA-dependent; negative regulation of transcription factor activity; histone deacetylation; osteoblast development; chromatin remodeling; positive regulation of protein sumoylation; regulation of protein binding; B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of transcription, DNA-dependent