Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation (0.1ug, 0.5ug, 1.0ug of Anti-ERK1) using 50ug of HEK-293 cell lysate.)
Mouse ERK1 Monoclonal Antibody | anti-ERK1 antibody
Anti-ERK1
Reactivity
Immunoprecipitate ERK1 from HumanERK1 from other species may also be detectable.
Applications
Immunoprecipitation
Purity
Affinity purified using Protein G
Synonyms
ERK1; Monoclonal Antibody; Anti-ERK1; anti-ERK1 antibody
Host
Mouse
Reactivity
Immunoprecipitate ERK1 from Human
ERK1 from other species may also be detectable.
ERK1 from other species may also be detectable.
Clonality
Monoclonal
Isotype
Mouse, IgG2b
Clone Number
E40
Specificity
Recognizes only the ERK1 protein
Purity/Purification
Affinity purified using Protein G
Form/Format
10mM PBS pH7.2
Applicable Applications for anti-ERK1 antibody
Immunoprecipitation (IP)
Application Notes
IP: 1ug
Immunogen
Recombinant full length ERK1 protein
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation (0.1ug, 0.5ug, 1.0ug of Anti-ERK1) using 50ug of HEK-293 cell lysate.)
Immunoprecipitation (IP)
(Sample Immunoprecipitation: Representative immunoprecipitation (0.1ug, 0.5ug, 1.0ug of Anti-ERK1) using 50ug of HEK-293 cell lysate.)
Related Product Information for anti-ERK1 antibody
ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs) which are activated in response to numerous growth factors and cytokines (1). Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos, and C/EBP beta).
Product Categories/Family for anti-ERK1 antibody
References
1. Boulton, TG. et al: Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase. Biochemistry. 1991 Jan 8;30(1):278-86.
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Product Notes
The ERK1 (Catalog #AAA516083) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ERK1 reacts with Immunoprecipitate ERK1 from Human ERK1 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's ERK1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). IP: 1ug. Researchers should empirically determine the suitability of the ERK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ERK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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