Rabbit anti-Human PDCD4 Polyclonal Antibody | anti-PDCD4 antibody

PDCD4, CT (Programmed Cell Death Protein 4, Neoplastic Transformation Inhibitor Protein, Nuclear Antigen H731-like, Protein 197/15a, H731)

Cat. Num. AAA648272

• Gene Names: PDCD4; H731
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PDCD4; Polyclonal Antibody; CT (Programmed Cell Death Protein 4; Neoplastic Transformation Inhibitor Protein; Nuclear Antigen H731-like; Protein 197/15a; H731); Anti -PDCD4; anti-PDCD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human PDCD4. Species sequence homology: frog, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.02M PBS, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

• Applicable Applications for anti-PDCD4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:10,000-1:50,000; Western Blot: 1:1000-1:10,000; Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human PDCD4 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Skin (formalin-fixed, paraffin-embedded) stained with PDCD4 antibody at 10ug/ml followed by biotinylated goat rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PDCD4 antibody

This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified. PDCD4 inhibits the translation initiation factor E1F4A. Phosphorylation by AKT causes nuclear translocation of PDCD4 and also decreases the ability of PDCD4 to interfere with the transactivation of AP-1 responsive promoter by c-JUN. Loss of PDCD4 expression in human lung carcinoma correlates with tumor progression and prognosis.

Product Categories/Family for anti-PDCD4 antibody

Antibodies; Abs to Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 313760537
• NCBI GeneID: 27250
• NCBI Accession #: NP_001186421.1
• NCBI GenBank Nucleotide #: NM_001199492.1
• UniProt Accession #: Q53EL6; O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91
• Molecular Weight: 51,735 Da
• NCBI Official Full Name: programmed cell death protein 4 isoform 3
• NCBI Official Synonym Full Names: programmed cell death 4 (neoplastic transformation inhibitor)
• NCBI Official Symbol: PDCD4
• NCBI Official Synonym Symbols: H731
• NCBI Protein Information: programmed cell death protein 4; protein 197/15a; nuclear antigen H731; neoplastic transformation inhibitor protein
• UniProt Protein Name: Programmed cell death protein 4
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD4
• UniProt Synonym Gene Names: H731
• UniProt Entry Name: PDCD4_HUMAN

NCBI Description

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

Function: Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA

By similarity. Ref.10 Ref.13 Ref.15 Ref.22 Ref.23 Ref.24

Subunit structure: Interacts (via MI domains) with EIF4A2

By similarity. Interacts (via MI domains) with EIF4A1 (via N-terminal domain). Heterotrimer with EIF4A1; one molecule of PDCD4 binds two molecules of EIF4A1. Interacts with EIF4G1. May form a complex with EIF4A1 and EIF4G1. The interaction between PDCD4 and EIF4A1 interferes with the interaction between EIF4A1 and EIF4G. When phosphorylated, interacts with BTRC and FBXW11. Ref.15 Ref.22 Ref.23 Ref.24

Subcellular location: Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. Ref.7 Ref.10

Tissue specificity: Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. Ref.7 Ref.8 Ref.13

Induction: IL2/interleukin-2 stimulation inhibits expression, while IL12/interleukin-12 increases expression. Ref.2

Domain: Binds EIF4A1 via both MI domains. Ref.22 Ref.24

Post-translational modification: Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.Phosphorylated at Ser-67 by RPS6KB1 in response to mitogens; phosphorylation promotes proteasomal degradation of PDCD4. Ref.10 Ref.15

Sequence similarities: Belongs to the PDCD4 family.Contains 2 MI domains.

Sequence caution: The sequence AAB42218.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAH15036.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.