Western Blot (WB)
(Sample Data: Representative western blot with Anti-ADD1 (1:1000) using 3 ug, 10 ug and 30 ug of HEK-293T cell lysate.)
Rabbit ADD1 Polyclonal Antibody | anti-ADD1 antibody
Anti-ADD1
Reactivity
Western blot ADD1 from human, mouse cellsADD1 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity chromatography
Synonyms
ADD1; Polyclonal Antibody; Anti-ADD1; anti-ADD1 antibody
Host
Rabbit
Reactivity
Western blot ADD1 from human, mouse cells
ADD1 from other species may also be detectable.
ADD1 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the ADD1 protein
Purity/Purification
Affinity chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-ADD1 antibody
Western Blot (WB)
Application Notes
WB: 21574:22573 dilution
Immunogen
Synthetic peptide corresponding to amino acids 724-728 of human ADD1 (P35611)
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot with Anti-ADD1 (1:1000) using 3 ug, 10 ug and 30 ug of HEK-293T cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-ADD1 (1:1000) using 3 ug, 10 ug and 30 ug of HEK-293T cell lysate.)
Related Product Information for anti-ADD1 antibody
ADD1 or Adducin 1 is a heterodimeric protein associated with the erythrocyte membrane skeleton, which promotes binding of spectrin to actin, and is a substrate for PKC and PKA (1). Alpha-and gamma-adducins are ubiquitously expressed while beta-adducin is expressed at high levels in brain and hematopoietic tissues. ADD1 can completely block elongation and depolymerization at the fast-growing barbed ends of actin filaments in vitro and this suggests that adducin restrict actin filament length in erythrocytes (2).
Product Categories/Family for anti-ADD1 antibody
References
1. Gardner, K. et.al: A new erythrocyte membrane-associated protein with calmodulin binding activity: identification and purification. J. Biol. Chem. 261: 1339-1348, 1986.
2. Kuhlman. et.al: A new functions for adducin: calcium/calmodulin-regulated capping of the barbed ends of actin filaments. J. Biol. Chem. 271: 7986-7991, 1996.
2. Kuhlman. et.al: A new functions for adducin: calcium/calmodulin-regulated capping of the barbed ends of actin filaments. J. Biol. Chem. 271: 7986-7991, 1996.
Similar Products
Product Notes
The ADD1 (Catalog #AAA515835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ADD1 reacts with Western blot ADD1 from human, mouse cells ADD1 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's ADD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 21574:22573 dilution. Researchers should empirically determine the suitability of the ADD1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ADD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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