Western Blot (WB)
(Anti- SP4 Picoband antibody, MBS177793, Western blottingAll lanes: Anti SP4 (MBS177793) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: U87 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 82KDObserved bind size: 82KD )
SP4 Polyclonal Antibody | anti-SP4 antibody
Anti-SP4 Antibody
Gene Names
SP4; HF1B; SPR-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
SP4; Polyclonal Antibody; Anti-SP4 Antibody; Transcription factor Sp4; HF1B; MGC130008; MGC130009; Sp4; SP4_HUMAN; SPR-1; SPR1; Sp4 transcription factor; anti-SP4 antibody
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
784
Applicable Applications for anti-SP4 antibody
Western Blot (WB)
Application Notes
WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat
Tested Species: In house tested species with positives results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Supported by chemiluminescent Kit MBS176460 in WB.
Tested Species: In house tested species with positives results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Supported by chemiluminescent Kit MBS176460 in WB.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human SP4 (592-620aa AVSPDQLTQVHLQQGQQTSDQEVQPGKRL), different from the related mouse sequence by one amino acid.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- SP4 Picoband antibody, MBS177793, Western blottingAll lanes: Anti SP4 (MBS177793) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: U87 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 82KDObserved bind size: 82KD )
Western Blot (WB)
(Anti- SP4 Picoband antibody, MBS177793, Western blottingAll lanes: Anti SP4 (MBS177793) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: U87 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 82KDObserved bind size: 82KD )
Related Product Information for anti-SP4 antibody
SP4(Sp4 transcription factor), also known as Transcription factor Sp4, MGC130008, MGC130009, SPR-1, HF1B, is a protein that in humans isencoded by the SP4 gene. It is a multigene family of SP proteins. Northern blot analysis that SP4 is abundantly expressed as a 7.5-kb messagein brain, but barely detectable in other human cell lines. SP4 is an activator protein like SP1. However, in contrast to SP1, SP4 is not able to actsynergistically through adjacent binding sites.
References
1. Hagen, G., Muller, S., Beato, M., Suske, G. Cloning by recognition site screening of two novel GT box binding proteins: a family of Sp1 related genes. Nucleic Acids Res. 20: 5519-5525, 1992. 2. Hagen, G., Dennig, J., Preiss, M., Beato, M., Suske, G.Functional analyses of the transcription factor Sp4 reveal properties distinct from Sp1 and Sp3. J. Biol. Chem. 270: 24989-24994, 1995.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transcription factor Sp4
NCBI Official Synonym Full Names
Sp4 transcription factor
NCBI Official Symbol
SP4
NCBI Official Synonym Symbols
HF1B; SPR-1
NCBI Protein Information
transcription factor Sp4
UniProt Protein Name
Transcription factor Sp4
Protein Family
UniProt Gene Name
SP4
UniProt Entry Name
SP4_HUMAN
Uniprot Description
SP4: Binds to GT and GC boxes promoters elements. Probable transcriptional activator. Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: Transcription factor; C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 7p15.3
Cellular Component: cytoplasm; intercellular bridge; microtubule cytoskeleton; nucleoplasm; nucleus
Molecular Function: DNA binding; metal ion binding; protein binding; transcription coactivator activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent
Research Articles on SP4
Similar Products
Product Notes
The SP4 sp4 (Catalog #AAA177793) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-SP4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SP4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat Tested Species: In house tested species with positives results. Other applications have not been tested. Optimal dilutions should be determined by end users. Supported by chemiluminescent Kit MBS176460 in WB. Researchers should empirically determine the suitability of the SP4 sp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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