Rabbit anti-Human TULP1 Polyclonal Antibody | anti-TULP1 antibody

TULP1, ID (TULP1, TUBL1, Tubby-related protein 1, Tubby-like protein 1) (Biotin)

Cat. Num. AAA6360279

• Gene Names: TULP1; RP14; LCA15; TUBL1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TULP1; Polyclonal Antibody; ID (TULP1; TUBL1; Tubby-related protein 1; Tubby-like protein 1) (Biotin); Tubby-like protein 1; anti-TULP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TULP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TULP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 183-211 amino acids from the Central region of human TULP1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TULP1 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the TULP1 antibody detected the TULP1 protein (arrow).)

Related Product Information for anti-TULP1 antibody

This gene encodes a member of the tubby-like gene family (TULPs). Members of this family have been identified in plants, vertebrates, and invertebrates and encode proteins of unknown function. TULP proteins share a conserved C-terminal region of approximately 200 amino acid residues. Mutations in this gene may be associated with retinitis pigmentosa.

Product Categories/Family for anti-TULP1 antibody

Antibodies; Proteins

References

Clark, G.R., et al. Ophthalmology (2010). In press: Caberoy, N.B., et al. Exp. Cell Res. 316(2):245-257(2010). Abbasi, A.H., et al. Mol. Vis. 14, 675-682 (2008): Mataftsi, A., et al. Invest. Ophthalmol. Vis. Sci. 48(11):5160-5167(2007). den Hollander, A.I., et al. Arch. Ophthalmol. 125(7):932-935(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 157266334
• NCBI GeneID: 7287
• NCBI Accession #: NP_003313.3
• NCBI GenBank Nucleotide #: NM_003322.3
• UniProt Accession #: O00294; O43536; Q5TGM5; Q8N571; Q0QD38; Q5TGM7; Q5TGM8
• Molecular Weight: 60,609 Da
• NCBI Official Full Name: tubby-related protein 1
• NCBI Official Synonym Full Names: tubby like protein 1
• NCBI Official Symbol: TULP1
• NCBI Official Synonym Symbols: RP14; LCA15; TUBL1
• NCBI Protein Information: tubby-related protein 1; OTTHUMP00000016266; OTTHUMP00000016267
• UniProt Protein Name: Tubby-related protein 1
• Protein Family: Tubby-related protein
• UniProt Gene Name: TULP1
• UniProt Synonym Gene Names: TUBL1
• UniProt Entry Name: TULP1_HUMAN

Uniprot Description

TULP1: Required for normal development of photoreceptor synapses. Required for normal photoreceptor function and for long- term survival of photoreceptor cells. Interacts with cytoskeleton proteins and may play a role in protein transport in photoreceptor cells. Binds lipids, especially phosphatidylinositol 3-phosphate, phosphatidylinositol 4- phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 4,5-bisphosphate, phosphatidylinositol 3,4,5-bisphosphate, phosphatidylserine and phosphatidic acid (in vitro). Contribute to stimulation of phagocytosis of apoptotic retinal pigment epithelium (RPE) cells and macrophages. Defects in TULP1 are the cause of retinitis pigmentosa type 14 (RP14). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP14 inheritance is autosomal recessive. Defects in TULP1 are the cause of Leber congenital amaurosis type 15 (LCA15). LCA15 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the TUB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: photoreceptor inner segment; cell projection; photoreceptor outer segment; cytoplasm; plasma membrane; extracellular region; synapse; cell junction

Molecular Function: actin filament binding; protein binding; phosphatidylinositol-4,5-bisphosphate binding; G-protein coupled photoreceptor activity

Biological Process: retinal homeostasis; eye photoreceptor cell development; visual perception; retina development in camera-type eye; positive regulation of phagocytosis; photoreceptor cell maintenance; dendrite development; phototransduction; detection of light stimulus involved in visual perception; phagocytosis

Disease: Retinitis Pigmentosa 14; Leber Congenital Amaurosis 15