Aquaporin 4 Polyclonal Antibody | anti-AQP4 antibody

Anti-Aquaporin 4 Antibody

Cat. Num. AAA178038

• Gene Names: AQP4; MIWC; WCH4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: Aquaporin 4; Polyclonal Antibody; Anti-Aquaporin 4 Antibody; Aquaporin-4; AQP 4; AQP-4; AQP4; AQP4_HUMAN; Aquaporin type 4; Aquaporin4; HMIWC 2; HMIWC2; Mercurial insensitive water channel; Mercurial-insensitive water channel; MGC22454; MIWC; WCH 4; WCH4; aquaporin 4; anti-AQP4 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃
• Sequence Length: 323

• Applicable Applications for anti-AQP4 antibody: Western Blot (WB), Immunohistochemistry (IHC) Frozen/Paraffin
• Application Notes: Western Blot:; Concentration: 0.1-0.5ug/ml; Tested Species: Mouse, Rat; Predicted Species: Human; ; Immunohistochemistry (Paraffin-embedded Section):; Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat; ; Immunohistochemistry (Frozen Section):; Concentration: 0.5-1ug/ml; Tested Species: Mouse, Rat; Predicted Species: Human; ; Tested Species: In-house tested species with positive results; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20mins is required for the staining of formalin/paraffin sections.; ; Other applications have not been tested.; ; Optimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 4 (295-323aa KPGVVHVIDVDRGEEKKGKDQSGEVLSSV), different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Recommended Detection Systems: Our lab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (MBS176460) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (MBS176453) for IHC-P.
• Preparation and Storage: Store at -20°C for one year. After reconstitution , store at 4°C for one month. It can also be aliquotted and stored at -20°C for six months.; Avoid repeated freezing and thawing.

Western Blot

(Anti- Aquaporin 4 Picoband antibody, MBS178038 Western blottingAll lanes: Anti Aquaporin 4 (MBS178038) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 35KDObserved bind size: 45KD)

Immunohistochemistry

(Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(P)IHC(P): Mouse Brain Tissue)

Immunohistochemistry

(Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(P)IHC(P): Rat Brain Tissue)

Immunohistochemistry

(Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(P)IHC(P): Human Glioma Tissue)

Immunohistochemistry

(Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(F)IHC(F): Mouse Brain Tissue)

Immunohistochemistry

(Anti-Aquaporin 4 Picoband antibody, MBS178038, IHC(F)IHC(F): Rat Brain Tissue)

Related Product Information for anti-AQP4 antibody

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provides a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92 amino acids separated by introns of 0.8, 0.3, and 5.2 kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7 kb upstream of exon 1. When spliced together, M1 and the subsequent 10 amino acids are encoded by exon 0; the next 11 amino acids and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. And AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.

References

1. Amiry-Moghaddam, M.; Otsuka, T.; Hurn, P. D.; Traystman, R. J.; Haug, F.-M.; Froehner, S. C.; Adams, M. E.; Neely, J. D.; Agre, P.; Ottersen, O. P.; Bhardwaj, A. : An alpha-syntrophin-dependent pool of AQP4 in astroglial end-feet confers bidirectional water flow between blood and brain. Proc. Nat. Acad. Sci. 100: 2106-2111, 2003. 2. Agre P, Nielsen S (1996). "The aquaporin family of water channels in kidney". Nephrologie 17 (7): 409-15. 3. Lennon, V. A.; Kryzer, T. J.; Pittock, S. J.; Verkman, A. S.; Hinson, S. R. : IgG marker of optic-spinal multiple sclerosis binds to the aquaporin-4 water channel. J. Exp. Med. 202: 473-477, 2005. 4. Lu, M.; Lee, M. D.; Smith, B. L.; Jung, J. S.; Agre, P.; Verdijk, M. A. J.; Merkx, G.; Rijss, J. P. L.; Deen, P. M. T. : The human AQP4 gene: definition of the locus encoding two water channel polypeptides in brain. Proc. Nat. Acad. Sci. 93: 10908-10912, 1996. 5. Nagelhus EA, Mathiisen TM, Ottersen OP (2004). "Aquaporin-4 in the central nervous system: cellular and subcellular distribution and coexpression with KIR4.1". Neuroscience 129 (4): 905-13.

NCBI and Uniprot Product Information

• NCBI GI #: 4502181
• NCBI GeneID: 361
• NCBI Accession #: NP_001641.1
• NCBI GenBank Nucleotide #: NM_001650.5
• UniProt Accession #: P55087; P78564
• NCBI Official Full Name: aquaporin-4 isoform M1
• NCBI Official Synonym Full Names: aquaporin 4
• NCBI Official Symbol: AQP4
• NCBI Official Synonym Symbols: MIWC; WCH4
• NCBI Protein Information: aquaporin-4
• UniProt Protein Name: Aquaporin-4
• Protein Family: Aquaporin
• UniProt Gene Name: AQP4
• UniProt Synonym Gene Names: AQP-4; MIWC
• UniProt Entry Name: AQP4_HUMAN

NCBI Description

This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2015]

Uniprot Description

AQP4: a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The predominant aquaporin found in brain. Two alternatively spliced transcript variants have been described

Protein type: Transporter, aquaporin family; Transporter; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 18q11.2-q12.1

Cellular Component: basolateral plasma membrane; cytoplasm; external side of plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: glycerol channel activity; water channel activity; water transporter activity

Biological Process: cellular water homeostasis; glycerol transport; multicellular organismal water homeostasis; renal water homeostasis; transport; water transport